Tumor antigen detection kit and tumor antigen detection method

A technology for tumor antigen and detection method, which is applied in the detection kit for tumor antigen and the detection field thereof

Inactive Publication Date: 2018-02-09
北京金沐医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • Tumor antigen detection kit and tumor antigen detection method
  • Tumor antigen detection kit and tumor antigen detection method
  • Tumor antigen detection kit and tumor antigen detection method

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Embodiment 1

[0074] A method for detecting tumor antigens, comprising the steps of:

[0075] Prepare the following solutions:

[0076] Lysis solution A: 10±0.5mM Tris-HCl (pH 7.5), 10±0.5mM NaH 2 PO 4 , 130±5mM NaCl, 1%±0.05% Triton X-100 and 10±0.5mM NaCl 4 P 2 o 7 ;

[0077] Lysis solution B: 150±5mM NaCl, 0.1%±0.005% Triton X-100, 1±0.05mM EDTA, 20±0.1mM HEPES and 10%±0.5% glycerol;

[0078] Carbonate coating buffer: Na 2 CO 3 and NaHCO 3 Aqueous solution of which, by volume of water, Na 2 CO 3 The concentration is 0.159g / L, NaHCO 3 The concentration is 0.293g / L;

[0079] The blocking solution is PBS containing 0.8% BSA by volume;

[0080] 1. Take 100 μL of blood sample, add 2 mL of Lysis Solution A, vortex to mix, incubate for 15 min, and lyse red blood cells;

[0081] 2. Centrifuge at 800g for 5min, remove the supernatant, pour off the supernatant slowly, absorb the residual liquid with paper, and vortex the cells;

[0082] 3. Add 2ml PBS to wash, vortex to mix, centrif...

Embodiment 2

[0094] A method for detecting tumor antigens, comprising the steps of:

[0095] Prepare the following solutions:

[0096] Lysis solution A: 10±0.5mM Tris-HCl (pH 7.4), 10±0.5mM NaH 2 PO 4 , 130±5mM NaCl, 1%±0.05% Triton X-100 and 10±0.5mM NaCl 4 P 2 o 7 ;

[0097] Lysis solution B: 150±5mM NaCl, 0.1%±0.005% Triton X-100, 1±0.05mM EDTA, 20±0.1mM HEPES and 10%±0.5% glycerol;

[0098] Carbonate coating buffer: Na 2 CO 3 and NaHCO 3 Aqueous solution of which, by volume of water, Na 2 CO 3 The concentration is 0.154g / L, NaHCO 3 The concentration is 0.29g / L;

[0099] The blocking solution is PBS containing 1% BSA by volume;

[0100] 1. Take 200 μL of blood sample, add 3 mL of lysate A, vortex to mix, incubate for 10 min, and lyse the red blood cells;

[0101] 2. Centrifuge at 750g for 6min, remove the supernatant, pour off the supernatant slowly, absorb the residual liquid with paper, and vortex the cells;

[0102] 3. Add 3ml PBS to wash, vortex to mix, centrifuge at ...

Embodiment 3

[0114] A method for detecting tumor antigens, comprising the steps of:

[0115] Prepare the following solutions:

[0116] Lysis solution A: 10±0.5mM Tris-HCl (pH 7.6), 10±0.5mM NaH 2 PO 4 , 130±5mM NaCl, 1%±0.05% Triton X-100 and 10±0.5mM NaCl 4 P 2 o 7 ;

[0117] Lysis solution B: 150±5mM NaCl, 0.1%±0.005% Triton X-100, 1±0.05mM EDTA, 20±0.1mM HEPES and 10%±0.5% glycerol;

[0118] Carbonate coating buffer: Na 2 CO 3 and NaHCO 3 Aqueous solution of which, by volume of water, Na 2 CO 3 The concentration is 0.165g / L, NaHCO 3 The concentration is 0.30g / L;

[0119] The blocking solution is PBS containing 1.2% BSA by volume;

[0120] 1. Take 100 μL of blood sample, add 2 mL of Lysis Solution A, vortex to mix, incubate for 20 min, and lyse red blood cells;

[0121] 2. Centrifuge at 850g for 4min, remove the supernatant, pour off the supernatant slowly, absorb the residual liquid with paper, and vortex the cells;

[0122] 3. Add 2ml PBS to wash, vortex to mix, centrifu...

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Abstract

The present invention relates to the field of tumor antigen level detection, particularly to a tumor antigen detection kit and a tumor antigen detection method. The tumor antigen detection kit comprises an antibody 1 and a substance for displaying the antibody 1, and/or an antibody 2 and a substance for displaying the antibody 2, wherein the antigen of the antibody 1 is TKTL1, and the antigen of the antibody 2 is DNASE1L1. The present invention further provides a tumor antigen detection method, wherein the tumor antigen information is obtained by detecting TKTL1 and DNASE1L1 in leukocyte lysates in the blood. According to the present invention, the tumor antigen level in the blood is obtained by detecting the TKTL1 (transketolase-sample 1) and the DNASE1L1 (deoxyribonuclease 1-sample 1) inthe blood, such that the detection results are stable, the specificity is high, and the detection is convenient.

Description

technical field [0001] The invention relates to the field of detection of tumor antigen levels, in particular to a kit for detecting tumor antigens and a detection method thereof. Background technique [0002] Monocytes / macrophages are an important part of the cells of the human immune system. Human peripheral blood cells can be mainly divided into anucleated cells (mainly red blood cells) and nucleated cells (white blood cells, etc.). Monocytes account for about 5% of the total number of nuclear cells and are differentiated from myeloid hematopoietic progenitor cells. They are important components of antigen-presenting cells (APC cells) in the immune system. Its maturation and differentiation in the human circulatory system and the realization of immunological functions can be roughly divided into the following steps: monocytes in capillaries → enter extravascular tissues through the vessel wall → discover heterologous substances (bacteria, pathogens, tumor cells, etc. ),...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N33/573
CPCG01N33/574G01N33/573G01N2333/9104G01N2333/922
Inventor 郭驰张小俊杜朝阳
Owner 北京金沐医疗科技有限公司
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