Method for simultaneous determination of rabeprazole sodium and metabolites thereof in Beagle dog plasma

Abstract

The invention relates to a method for simultaneous determination of levorabeprazole sodium, dexrabeprazole sodium and rabeprazole sodium metabolites comprising demethylated rabeprazole sodium, thioether rabeprazole sodium and rabeprazole sodium sulphone in Beagle dog plasma by using LC / MS / MS, and belongs to the field of biological detection. In experiments, phenacetin is used as an internal standard, and after ethyl acetate extraction pretreatment is performed, a high performance liquid chromatography-tandem mass spectrometry is adopted for simultaneous determination of the blood drug concentrations of levorabeprazole sodium, dexrabeprazole sodium and three metabolites of rabeprazole sodium in the dog plasma. The method has the advantages of high specificity, high sensitivity and simple operation, and overcomes the limitation of batch test by using two instruments, and is successfully applied to analysis and study of optical isomers of rabeprazole sodium and the metabolites thereof inthe Beagle dog plasma.

Description

technical field [0001] The invention relates to a method for simultaneously determining left and right rabeprazole sodium and rabeprazole sodium metabolites desmethyl rabeprazole sodium and sulfide rabeprazole sodium in Beagle dog plasma by using LC / MS / MS The invention discloses a detection method for rabeprazole sodium sulfone and belongs to the technical field of biological detection. Background technique [0002] Peptic ulcer (peptic ulcer) is an ulcer caused by the digestion of mucous membranes by acidic gastric juice. It mainly refers to chronic ulcers that occur in the stomach and duodenum. It is a frequently-occurring, chronic, and common disease. If it is not well controlled, it will lead to gastric bleeding, gastric perforation and canceration. [0003] Rabeprazole sodium (Sodium rabeprazole) is a new type of benzimidazole proton pump inhibitor, without anticholinergic and anti-H 2 Histamine properties, but can attach to parietal cell surface by inhibiting H + / K...

AI Technical Summary

Problems solved by technology

[0005] At present, the system-validated HPLC-MS / MS method can only use two instruments to detect rabeprazole sodium optical isomers and their metabolites in batches. The bibliographical reports of beprazole sodium metabolites desmethylrabeprazole sodium, sulfide rabeprazole sodium and rabeprazole sodium sulfone, the present invention establishes for the first time to utilize LC / MS / MS to simultaneously measure Beagle dog plasma The detection method of left-handed and right-handed rabeprazole sodium and rabeprazole sodium metabolite desmethylrabeprazole sodium, sulfide rabeprazole sodium and rabeprazole sodium sulfone is rabeprazole sodium The detection of preclinical blood drug concentration has laid a solid methodological foundation

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