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Chemiluminescent kit for rapid diagnosis of early and middle stages of breast cancer

A chemiluminescent reagent and rapid diagnosis technology, which is applied in the fields of chemiluminescence/bioluminescence, analysis through chemical reaction of materials, scientific instruments, etc., can solve the difficulty in meeting the requirements of early rapid diagnosis and affect the early detection and treatment of breast cancer and other issues to achieve the effect of strong accuracy and high sensitivity

Inactive Publication Date: 2018-02-16
GUANGZHOU HENGTAI BIOTECH
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  • Application Information

AI Technical Summary

Problems solved by technology

Because the single detection of selected tumor markers often has great limitations, it is difficult to meet the requirements of early and rapid diagnosis
[0004] At present, there is no rapid and efficient diagnostic kit for breast cancer on the market, which seriously affects the early detection and treatment of breast cancer

Method used

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  • Chemiluminescent kit for rapid diagnosis of early and middle stages of breast cancer
  • Chemiluminescent kit for rapid diagnosis of early and middle stages of breast cancer
  • Chemiluminescent kit for rapid diagnosis of early and middle stages of breast cancer

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Embodiment Construction

[0029] The following describes in detail the chemiluminescent kit for the rapid diagnosis of early and mid-stage breast cancer in conjunction with the accompanying drawings and specific examples.

[0030] A chemiluminescent kit for rapid diagnosis of early and mid-stage breast cancer, including capture antibody, capture antibody blocking buffer, standard, detection antibody labeled with HRP, washing solution and chromogenic solution, etc. The tumor marker components include: galectin, sugar chain antigen 15-3 and human aspartyl β-hydroxylase. The capture antibodies include monoclonal antibodies prepared separately from the three markers; the kit includes tumor marker standards, microwell plates coated with tumor marker monoclonal capture antibodies, horseradish peroxidase or alkaline phosphate Enzyme-labeled tumor marker monoclonal detection antibody, chemiluminescence substrate solution, concentrated washing solution. The horseradish peroxidase chemiluminescent substrate sol...

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Abstract

The invention discloses a chemiluminescent kit for rapid diagnosis of early and middle stages of breast cancer; tumor markers comprise the components: galectin, a carbohydrate antigen 15-3 and human asparagine beta-hydroxylase; the chemiluminescent kit comprises a tumor marker standard substance, a tumor marker monoclonal capture antibody coated micropore plate, horse radish peroxidase or alkalinephosphatase labeled tumor marker monoclonal detection antibody, a chemiluminescent substrate solution and a concentrated washing liquid. The chemiluminescent kit has the following beneficial effects:the chemiluminescent kit has the advantages of high sensitivity and strong accuracy, and the sensitivity and the accuracy both reach 90% or more; and besides, the chemiluminescent kit can effectivelyreduce the detection cost, reduces operation steps and reduces detection errors.

Description

Technical field: [0001] The invention relates to the field of kits for cancer diagnosis and detection, in particular to a chemiluminescence kit for rapid diagnosis of breast cancer in the early and middle stages. Background technique: [0002] Breast cancer is the most common cancer in women all over the world, and about 500,000 people die of breast cancer every year in the world. In European and American countries, on average, one out of every four female cancer patients suffers from breast cancer, which shows that the prevalence rate is extremely high. The most common age of onset in Eastern women is 40 to 50 years old, while that of Western women is 30 to 40 years old. According to statistics, in recent years, the growth rate of the incidence of breast cancer in my country is 1-2 percentage points higher than that of high-incidence countries. According to the data released by the National Cancer Center and the Bureau of Disease Control and Prevention of the Ministry of ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N33/531G01N21/76
CPCG01N33/57415G01N21/76G01N33/531G01N33/57484
Inventor 余跃飞
Owner GUANGZHOU HENGTAI BIOTECH