Vitamin premix feed as well as preparation method and use method thereof
A technology of premixed feed and vitamins, which is applied in animal feed, animal feed, application and other directions, can solve the problems of slow onset, poor stability, and difficult absorption, and achieves the effects of reasonable material selection, low manufacturing cost and simple method.
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Embodiment 1
[0031] The preparation of embodiment 1 bacillus subtilis
[0032] The bacillus subtilis of the embodiment of the present invention is to adopt following method to make:
[0033] (1) Slant culture: the slant medium prepared according to the following ratio: glucose 20g / L, peptone 15g / L, sodium chloride 1g / L, yeast extract 5g / L, agar 20g / L, pH 6.5; Bacillus subtilis The bacillus species were inoculated on the slant medium, and cultured for 30 hours at a temperature of 28°C to obtain the Bacillus subtilis species;
[0034] (2) primary seed culture: the primary seed medium prepared in the following ratio: glucose 20g / L, peptone 15g / L, sodium chloride 1g / L, yeast extract 5g / L, pH 6.5; Bacillus species were inoculated into the primary seed medium, and cultured for 30 hours at a temperature of 30°C and a rotational speed of 200r / min to obtain a primary liquid seed culture solution;
[0035] (3) secondary seed culture: the secondary seed culture medium prepared in the following rati...
Embodiment 2
[0039] The embodiment of the present invention provides a kind of preparation method of vitamin premixed feed, comprises the following steps:
[0040] (1) Each kilogram of vitamin premixed feed consists of the following raw materials: vitamin A 200,000 IU, vitamin D 3 100,000 IU, Vitamin E 10g, Vitamin K 3 2g, vitamin B 1 3g, vitamin B 2 5g, vitamin B 6 5g, vitamin B 12 10g, 10g of niacin, 5g of calcium pantothenate, 0.2g of folic acid, 50mg of biotin, 20g of inositol, 1g of astragalus polysaccharide, 1g of vitamin C phosphate, and the rest is the Bacillus subtilis powder obtained in Example 1 of the present invention.
[0041] (2) When the ambient temperature is 35°C and the relative humidity is ≤65%, add vitamin A and vitamin D to the stainless steel mixing tank 3 , Vitamin B 12 , folic acid, biotin and 10% of the Bacillus subtilis powder obtained in the embodiment of the present invention 1 stirred for 5 minutes to mix it homogeneously; other remaining vitamin E, ...
Embodiment 3
[0043] (1) Each kilogram of vitamin premixed feed consists of the following raw materials: vitamin A 300,000 IU, vitamin D 3 100,000 IU, Vitamin E 20g, Vitamin K 3 5g, vitamin B 1 5g, vitamin B 2 8g, vitamin B 6 10g, vitamin B 12 15g, niacin 15g, calcium pantothenate 10g, folic acid 0.5g, biotin 60mg, inositol 25g, astragalus polysaccharide 2g, vitamin C phosphate 2g, and the rest is the Bacillus subtilis powder obtained in Example 1 of the present invention.
[0044] (2) When the ambient temperature is 32°C and the relative humidity is ≤65%, add vitamin A and vitamin D to the stainless steel mixing tank 3 , Vitamin B 12 , folic acid, biotin and 10% of the Bacillus subtilis powder obtained in the embodiment of the present invention 1 stirred for 8 minutes to mix it homogeneously; other remaining vitamin E, vitamin D 3 , Vitamin B 1 , Vitamin B 2 , niacin, calcium pantothenate, vitamin B 6 , inositol, vitamin C and the remaining 90% Bacillus subtilis powder into a ...
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