Fluorescent quantitative PCR detection system used for nasopharyngeal carcinoma gene screening and applications of fluorescent quantitative PCR detection system

A fluorescence quantification and detection system technology, applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problems of low interpretation of genetic ratio, weak correlation of single locus, etc., to avoid inducing factors and improve lifestyle. , the effect of high detection rate

Inactive Publication Date: 2018-02-23
SHENZHEN MEIGENE MEDICAL LAB
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  • Application Information

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Problems solved by technology

[0010] Most of the existing research and detection of nasopharyngeal carcinoma is based on the relevant association loci obtained from the GWAS study of large samples of Chinese. However, when applied to the Chinese population, there i

Method used

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  • Fluorescent quantitative PCR detection system used for nasopharyngeal carcinoma gene screening and applications of fluorescent quantitative PCR detection system
  • Fluorescent quantitative PCR detection system used for nasopharyngeal carcinoma gene screening and applications of fluorescent quantitative PCR detection system
  • Fluorescent quantitative PCR detection system used for nasopharyngeal carcinoma gene screening and applications of fluorescent quantitative PCR detection system

Examples

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Effect test

Embodiment 1

[0057] In this example, a nasopharyngeal carcinoma fluorescent quantitative PCR detection kit was used to detect nasopharyngeal carcinoma-related genes, in which 7 polymorphic sites on six genes related to nasopharyngeal carcinoma were detected, including rs2212020, rs189897, rs9510787, rs6774494, rs1412829, rs401681 and rs6498114, a total of 7 loci. Using specifically designed primers and probes, the amplification system and conditions are optimized, and the detection of 7 polymorphic sites on six genes is completed simultaneously in the same system.

[0058] In this example, a single fluorescently labeled probe, Roche 480 platform, and finally detected rs2212020, rs189897, rs9510787, rs6774494, rs1412829, rs401681 and rs6498114 sites by melting curve peak pattern.

[0059] Specifically, in the dual fluorescent quantitative PCR detection method for rs2212020, rs189897, rs9510787, rs6774494, rs1412829, rs401681 and rs6498114 sites, the primer probes and primers for rs2212020...

Embodiment 2

[0088] The seven loci in Example 1 are from the articles published by Professor Bei Jinxin from China, Ching ChingNg from Japan, and their respective international cooperation teams. The articles have obtained the susceptibility loci directly related to nasopharyngeal carcinoma through GWAS research , and calculated the allelic risk value of the locus, that is, the OR value. GWAS research found that common mutations in multiple genes, including ITGA9 and TNFRSF19, may be related to the risk of nasopharyngeal carcinoma. Ching ChingNg found that the ITGA9 gene may be a new susceptibility gene for nasopharyngeal carcinoma, which encodes the integrin α9 subunit. At present, it is not clear about the occurrence and development of this gene and nasopharyngeal carcinoma, so it is likely to be affected by other genetic factors. Factors and environmental factors (such as herpes virus, Epstein-Barr virus, etc.). Professor Bei Jinxin and his research team did a large-scale GWAS study on ...

Embodiment 3

[0110] The method of the present invention extracts DNA by collecting the genetic sample of subject then, detects with Roche LightCycler480 real-time quantitative instrument, the result obtained can refer to figure 1 , 3, 5, 7, 9, 11, 13.

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Abstract

The invention relates to the technical field of biological detection, in particular relates to a fluorescent quantitative PCR detection kit, and further discloses applications of the fluorescent quantitative PCR detection kit in detecting nasopharyngeal carcinoma. A fluorescent quantitative PCR detection system comprises the detection kit for a specific site gene, wherein the score of the nasopharyngeal carcinoma risk is calculated according to the risk value of each site for nasopharyngeal carcinoma risk, crowd grading is carried out according to the existing database, the risk level of nasopharyngeal carcinoma of each person is obtained according to the personal score, so that the risk of nasopharyngeal carcinoma of the tested persons is evaluated, the high-risk people are guided to improve the living mode, the inducing factors are avoided, and the physical examination frequency is increased. Meanwhile, a Chinese population database is established according to the selected seven genepolymorphism sites, and thus a foundation is laid for establishing the specific nasopharyngeal carcinoma evaluation for the Chinese population.

Description

technical field [0001] The present invention relates to the technical field of biological detection, in particular to a fluorescent quantitative PCR detection system for nasopharyngeal cancer gene screening, and further discloses a method for nasopharyngeal cancer gene screening based on the fluorescent quantitative PCR detection system . Background technique [0002] With the continuous development of scientific research, especially after the completion of the Human Genome Project and the full development of the post-genome project, the application of the latest technology dominated by genetic testing in the field of disease prevention and control has attracted widespread attention. At present, preliminary progress has been made in the prediction of disease risk using genetic theories and methods. Commonly used genetic testing methods include: direct sequencing (DS), ligase detection reaction (LDR), restriction Fragment length polymorphism analysis (restriction fragment le...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886
CPCC12Q1/6851C12Q1/6886C12Q2600/156C12Q2563/107C12Q2531/113C12Q2537/165
Inventor 吴少鸿周文根李欣姜萍萍黄君任飞詹延延赵世林李政
Owner SHENZHEN MEIGENE MEDICAL LAB
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