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Construction method and application of a supramolecular hydrogel material

A supramolecular hydrogel and structural formula technology, applied in the field of new materials, can solve problems such as complex operating conditions, irreversibility, and undisclosed methods of constructing hybrid supramolecular hydrogel materials through electrostatic interactions

Active Publication Date: 2020-04-10
HUAIYIN TEACHERS COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the operation conditions are complex and irreversible through covalent bonding, and there is no method for constructing hybrid supramolecular hydrogel materials through electrostatic interaction.

Method used

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  • Construction method and application of a supramolecular hydrogel material
  • Construction method and application of a supramolecular hydrogel material
  • Construction method and application of a supramolecular hydrogel material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0096] 1. Preparation method

[0097] 1.1 Synthesis of glutamic acid-terminated polyethylene glycol

[0098] 1) 5g (0.5mmol) of polyethylene glycol (HO-PEG 227 -OH) and excess succinic anhydride in 250 mL of anhydrous dichloromethane, after 2 days of reaction at 45 ° C, the excess anhydride was removed by adding water and hydrolyzing it, followed by liquid separation and washing, and the organic layer was spin-dried to dry the solvent and vacuum-dried to obtain a Carboxylated polyethylene glycol (HOOC-PEG 227 -COOH);

[0099] 2) Take 3.0g of HOOC-PEG 227 -COOH, add 1.1 equivalents of N,N'-dicyclohexylcarbodiimide (0.133g), 1.05 equivalents of N-hydroxysuccinimide (0.071g), 0.01 equivalents of DMAP (0.72mg ), about 80 mL of anhydrous tetrahydrofuran, reacted in an ice-water bath for 5 minutes, and a certain amount of insoluble N, N'-dicycloethylurea (DCU) was produced. After reacting overnight, place in a refrigerator at 4° C. for about 1 hour, and filter to remove most of...

Embodiment 2

[0113] 1. Preparation method

[0114] 1.1 Synthesis of glutamic acid-terminated polyethylene glycol

[0115] 1) 3g (0.3mmol) of polyethylene glycol (HO-PEG 227 -OH) and excess succinic anhydride in 250 mL of anhydrous dichloromethane, after 3 days of reaction at 40 ° C, the excess anhydride was removed by adding water and hydrolyzing it, followed by liquid separation and washing, and the organic layer was spin-dried to dry the solvent and vacuum-dried to obtain a Carboxylated polyethylene glycol (HOOC-PEG 227 -COOH);

[0116] 2) Take 2.0g of HOOC-PEG 227 -COOH, add N,N'-dicyclohexylcarbodiimide (0.0824g), N-hydroxysuccinimide (0.0472g), DMAP (0.48mg), anhydrous tetrahydrofuran about 80mL, and react in an ice-water bath After 5 minutes, a certain amount of insoluble N,N'-dicycloethylurea (DCU) was produced. After reacting overnight, place in a refrigerator at 4° C. for about 1 hour, and filter to remove most of the DCU. After the filtrate was concentrated to two-thirds of...

Embodiment 3

[0130] 1. Preparation method

[0131] 1.1 Synthesis of glutamic acid-terminated polyethylene glycol

[0132] 1) 10g (1.0mmol) of polyethylene glycol (HO-PEG 227 -OH) and excess succinic anhydride in 250 mL of anhydrous dichloromethane, reacted at 50 °C for 1 day, and the excess anhydride was removed by adding water and hydrolyzing it, followed by liquid separation and washing, and the organic layer was spin-dried to dry the solvent and vacuum-dried to obtain a Carboxylated polyethylene glycol (HOOC-PEG 227 -COOH);

[0133] 2) Take 4.0g of HOOC-PEG 227 -COOH, N,N'-dicyclohexylcarbodiimide (0.177g), N-hydroxysuccinimide (0.0944g), DMAP (0.96mg), anhydrous tetrahydrofuran about 80mL, react in ice-water bath for 5min After that, a certain amount of insoluble N,N'-dicycloethylurea (DCU) was produced. After reacting overnight, place in a refrigerator at 4° C. for about 1 hour, and filter to remove most of the DCU. After the filtrate was concentrated to two-thirds of the origin...

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Abstract

The invention provides a construction method of a novel supramolecular hydrogel material and the application of the hydrogel material in tissue engineering, biomedical materials and drug loading. Polyethylene glycol and protidine DNA are prepared by electrostatic binding at a molar charge ratio of 2:(1-5). The method has the characteristics of simple operation, easy purification, high efficiency, low cost, and can maintain the activity of the original chiral center of the amino acid during the coupling process. The hydrogel material with supramolecular network structure prepared by this method can maintain the biological activity of the loaded molecules to the greatest extent, and realize local and sustained release of specific targets, and is biodegradable, reversible, and more environmentally friendly. Responsiveness and structural dynamic controllability.

Description

technical field [0001] The invention relates to the technical field of new materials, in particular to an Arg-based 2 -PEG-Arg 2 Supramolecular hydrogel materials constructed by electrostatic interaction with DNA, construction methods and applications. Background technique [0002] The hydrogel material is a gel material with water as the dispersion medium. It is a water-soluble polymer with a network cross-linked structure that introduces a part of hydrophobic groups and hydrophilic residues. The hydrophilic residues combine with water molecules to absorb water. A cross-linked polymer in which the molecules are connected within the network and the hydrophobic residues swell with water. Hydrogel materials constructed using polymers as basic raw materials can be roughly divided into two categories according to their gel formation mechanism, one is chemically cross-linked hydrogels formed by covalently linking polymers, and the other is chemically cross-linked hydrogels. Th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08G65/332C08G65/333A61K47/60A61K47/54A61K47/69A61L27/18A61L27/52
CPCA61L27/18A61L27/52C08G65/3328C08G65/33396C08G2650/04C08L71/02
Inventor 陈平王翔行岳真
Owner HUAIYIN TEACHERS COLLEGE