Am fungus-ramie joint remediation method of complex heavy metal polluted soil
A technology of combined restoration of contaminated soil, applied in the restoration of contaminated soil, etc., to achieve the effects of increasing ramie biomass, improving tolerance, and enhancing photosynthesis
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Embodiment 1
[0046] The method for AM fungus-ramie joint restoration of compound heavy metal polluted soil comprises the following steps:
[0047] (1) Get ramie seeds with 10% (v / v) H 2 o 2 Carry out immersion disinfection 25min, after disinfection, wash 4 times with tap water first, then wash 2 times with deionized water, and finally wrap the gauze wetted with deionized water under dark conditions to accelerate germination and plant in the non-polluting soil after autoclaving. When the ramie reaches the 10-leaf stage, use 10% (v / v) H 2 o 2 The size and swelling of the solution are similar to those of ramie seedlings, and they are rinsed 4 times with deionized water before being planted;
[0048] (2) Use a high-pressure steam sterilizer to sterilize the composite heavy metal soil for 2 hours, evenly sprinkle AM fungi with a density of 10g / kg soil, and cover 6cm of soil evenly on it, and water to make the soil moisture content reach 15 %;
[0049] (3) transplant the similar ramie see...
Embodiment 2
[0055] The method for AM fungus-ramie joint restoration of compound heavy metal polluted soil comprises the following steps:
[0056] (1) Get ramie seeds with 8% (v / v) H 2 o 2 Carry out immersion disinfection 20min, wash 3 times with tap water first after disinfection, then wash 3 times with deionized water, finally wrap the gauze that is moistened with deionized water under dark conditions and plant in the pollution-free soil after autoclaving, When the ramie reaches the 10-leaf stage, use 8% (v / v) H 2 o 2 The size and swelling of the solution are similar to ramie seedlings, and are rinsed 3 times with deionized water, and are ready to be planted;
[0057] (2) Use a high-pressure steam sterilizer to sterilize the composite heavy metal soil for 1.5h, evenly sprinkle AM fungi with a density of 7g / kg soil, and cover 5cm of soil evenly on it, water to make the soil moisture content reach 12%;
[0058] (3) transplant the similar ramie seedlings in the compound heavy metal p...
Embodiment 3
[0064] The method for AM fungus-ramie joint restoration of compound heavy metal polluted soil comprises the following steps:
[0065] (1) Get ramie seeds with 15% (v / v) H 2 o 2 Carry out immersion disinfection 30min, after disinfection, first wash 5 times with tap water, then wash 2 times with deionized water, and finally wrap the gauze wetted with deionized water under dark conditions to accelerate germination and plant in the non-polluting soil after autoclaving. When the ramie reaches the 10-leaf stage, use 15% (v / v) H 2 o 2 Solution rinsing size, swelling similar to ramie seedlings, and rinsing 5 times with deionized water, waiting to be planted;
[0066] (2) Use a high-pressure steam sterilizer to sterilize the composite heavy metal soil for 3 hours, evenly sprinkle AM fungi with a density of 13g / kg soil, and cover 7cm of soil evenly on it, and water to make the soil moisture content reach 18 %;
[0067] (3) transplanting similar ramie seedlings in the compound hea...
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