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Microfluidic centrifugal disc and blood coagulation detection method
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A microfluidic, disc technology, applied in the field of microfluidics, can solve the problems of complicated manual operation and human interference factors in the detection results, and achieve the effects of high detection efficiency, fast detection speed, and simple detection.
Pending Publication Date: 2018-03-13
ZHEJIANG PUSHKANG BIOTECHNOLOGY CO LTD
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Problems solved by technology
However, some small semi-automatic blood coagulation detection equipment has the problem of complicated manual operation and large human interference factors in the test results.
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Embodiment 1
[0030] figure 1 A schematic diagram of the structure of the microfluidic centrifugal disc in the embodiment of the present invention is given schematically, as shown in figure 1 As shown, the microfluidic centrifuge disc comprises:
[0031] At least two detection parts, the detection part includes:
[0032] Separation unit, described separation unit comprises:
[0033] An injection groove, the injection groove has a first injection hole; the end of the injection groove far away from the rotation center of the microfluidic centrifugal disk communicates with the third capillary through the first capillary;
[0034] The first storage tank, starting from the rotation center of the microfluidic centrifuge disc and along the radial direction of the microfluidic centrifuge disc, the injection tank, the first storage tank and the distribution unit are arranged in sequence; and Compared with the end of the first storage tank facing the second capillary, the end of the first storage ...
Embodiment 2
[0065] According to the application example of the microfluidic centrifugal disc in Example 1 of the present invention in coagulation detection of whole blood, five items of D dimer, APTT, FIB, PT, and TT are detected.
[0066] Such as figure 2 As shown, in this application example, the diameter of the microfluidic centrifugal disc is 120mm, the center is a through hole with a diameter of 10mm, and there is a semicircular gap on the outer edge of the circumference; there are 4 detection parts on the microfluidic centrifugal disc There are 5 quantitative tanks in the distribution unit of the detection part, corresponding to the 5 detection tanks 401, 402 in the detection unit, and the volume of the quantitative tank corresponding to the D dimer detection item is greater than the volume of other quantitative tanks; the reagent injection hole , The radius of rotation of the third storage tank is smaller than that of the first storage tank; on the circumferential side (non-radial...
Embodiment 3
[0079] As shown in the table below, the microfluidic centrifuge disc is matched with an existing instrument to obtain better detection results in a test. In this test, whole blood samples from the same patient were injected into the same microfluidic disk chip for testing. S1-S4 represent four different detection regions, and TT, PT, FIB, APTT, DD represent five different items. The result value of D-dimer is the concentration, and the result value of other items is the reaction time. AVE is the calculated average value, and CV is the coefficient of variation of each measured value.
[0080]
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Abstract
The invention provides a microfluidic centrifugal disc and a blood coagulation detection method; the microfluidic centrifugal disc comprises a separation unit, a distribution unit and a detection unit; the separation unit includes an injection channel having a first injection hole, and the end of the injection channel far away from the rotational center of the centrifugal disc is communicated witha third capillary through a first capillary; the injection channel, a first storage channel and the distribution unit are arranged in sequence from the rotational center of the centrifugal disc alongthe radius of the centrifugal disc; a first exhaust hole is communicated with the first storage channel through a second capillary; a second storage channel is communicated with the first storage channel through the third capillary; a third storage channel stores gas and is connected and communicated with the third capillary through a fourth capillary. Blood coagulation detection based on the microfluidic centrifugal disc features good simplicity, high efficiency and high speed and may cooperate with a full-automatic detection instrument to provide direct detection for a whole blood sample.
Description
technical field [0001] The invention relates to microfluidic technology, in particular to a microfluidic centrifugal disk and a blood coagulation detection method. Background technique [0002] Coagulation (Coagulation or clotting) is the process by which blood changes from liquid to gel. The process may cause hemostasis, which is the cessation of bleeding from damaged blood vessels, which is then repaired. The coagulation mechanism involves the activation, adhesion and aggregation of platelets, with the conversion of fibrinogen to fibrin, which is deposited and matured into a robust network. [0003] Currently popular coagulation detection methods are mostly tube coagulation detection and small semi-automatic detection. Most tube-type coagulation tests have the disadvantages of large amount of reagents, slow delivery of the first test result, and only plasma samples can be used. For projects with high reagent costs, reducing the amount of reagents used can significantly ...
Claims
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Application Information
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