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Method for preparing tobacco absolute oil by virtue of biological enzyme

A bio-enzyme and tobacco technology, applied in tobacco, tobacco treatment, application, etc., can solve problems such as single aroma style, low volatile or semi-volatile components, and large differences

Inactive Publication Date: 2018-03-16
昆明坤九香精香料有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Tobacco absolutes prepared by different processes have their own characteristics and uses, but the aroma of absolute extracted by conventional processes is quite different from the original aroma of tobacco, the volatile or semi-volatile components are low, and the aroma style is relatively single, which cannot fully adapt to the current tobacco-related products. development needs

Method used

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  • Method for preparing tobacco absolute oil by virtue of biological enzyme

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1. Sterilization: Weigh 1kg of tobacco powder or tobacco leaves, crush it to 20-80 mesh, add 500g of deionized water, stir and mix at 100 rpm, quickly heat to 85°C for 10 minutes to sterilize, and the sterilization time is up Finally, the system was quickly cooled to a constant temperature of 45°C with cold water.

[0023] 2. Enzymolysis: add 0.1-0.2% (W / W) compound enzyme preparation of the total weight of tobacco powder, adjust the pH to 5.5 with hydrochloric acid and sodium hydroxide, stir well, and the enzymolysis time is 1 hour, the enzyme activity of the compound enzyme preparation The ratio is as follows:

[0024] Pectinase 250-350U / ml

[0025] Cellulase 800-1000U / ml

[0026] Hemicellulase 500-600U / ml

[0027] α-amylase 800-1000U / ml

[0028] β-amylase 800-1000U / ml

[0029] Lipase 50-100U / ml

[0030] 3. Enzyme inactivation and extraction: transfer the enzymatically hydrolyzed tobacco powder into an extraction tank, add 95% edible alcohol at a weight ratio of...

Embodiment 2

[0033] 1. Sterilization: Weigh 1kg of tobacco powder or tobacco leaves, crush it to 20-80 mesh, add 500g of deionized water, stir and mix at 100 rpm, quickly heat to 85°C for 10 minutes to sterilize, and the sterilization time is up Finally, the system was quickly cooled to a constant temperature of 45°C with cold water.

[0034] 2. Enzymolysis: Add 0.1-0.2% (W / W) compound enzyme preparation of the total weight of tobacco powder, adjust the pH to 5.5 with hydrochloric acid and sodium hydroxide, stir well, and the enzymolysis time is 2 hours, the enzyme activity of the compound enzyme preparation The ratio is as follows:

[0035] Pectinase 250-350U / ml

[0036] Cellulase 800-1000U / ml

[0037] Hemicellulase 500-600U / ml

[0038] α-amylase 800-1000U / ml

[0039] β-amylase 800-1000U / ml

[0040] Lipase 50-100U / ml

[0041] 3. Enzyme inactivation and extraction: transfer the enzymatically hydrolyzed tobacco powder into an extraction tank, add 95% edible alcohol at a weight ratio o...

Embodiment 3

[0044] 1. Sterilization: Weigh 1kg of tobacco powder or tobacco leaves, crush it to 20-80 mesh, add 500g of deionized water, stir and mix at 100 rpm, quickly heat to 85°C for 10 minutes to sterilize, and the sterilization time is up Finally, the system was quickly cooled to a constant temperature of 45°C with cold water.

[0045] 2. Enzymolysis: Add 0.1-0.2% (W / W) compound enzyme preparation of the total weight of tobacco powder, adjust the pH to 5.5 with hydrochloric acid and sodium hydroxide, stir well, and the enzymolysis time is 3 hours, the enzyme activity of the compound enzyme preparation The ratio is as follows:

[0046] Pectinase 250-350U / ml

[0047] Cellulase 800-1000U / ml

[0048] Hemicellulase 500-600U / ml

[0049] α-amylase 800-1000U / ml

[0050] β-amylase 800-1000U / ml

[0051] Lipase 50-100U / ml

[0052] 3. Enzyme inactivation and extraction: transfer the enzymatically hydrolyzed tobacco powder into an extraction tank, add 95% edible alcohol at a weight ratio o...

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Abstract

The invention discloses a method for preparing tobacco absolute oil by virtue of a biological enzyme. According to the method, a tobacco raw material is treated by virtue of a complex enzyme preparation, alcohol extraction and petroleum ether redissolution are conducted, so that the absolute oil is prepared, wherein the complex enzyme preparation, in accordance with activity proportions, consistsof 250-350U / ml of pectase, 800-1000U / ml of cellulase, 500-600U / ml of hemicellulase, 800-1000U / ml of alpha-amylase, 800-1000U / ml of beta-amylase and 50-100U / ml of lipase. The finished absolute oil product is full of emitted aroma and is purer in natural tobacco fragrance, so that the absolute oil can adapt more to present tobacco demands.

Description

technical field [0001] The invention relates to a method for preparing tobacco spices, in particular to a method for preparing tobacco absolute by using various biological enzymes to treat tobacco dust and tobacco leaves. technical background [0002] Tobacco absolute is an irreplaceable raw material in cigarette flavoring, non-combustible tobacco and some cigarette auxiliary materials. It has a complex composition, full and pure aroma, and a comfortable aftertaste. Tobacco absolutes prepared by different processes have their own characteristics and uses, but the aroma of absolute extracted by conventional processes is quite different from the original aroma of tobacco, the volatile or semi-volatile components are low, and the aroma style is relatively single, which cannot fully adapt to the current tobacco-related products. development needs. Contents of the invention [0003] The purpose of the present invention is to provide a method for improving the extraction proces...

Claims

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Application Information

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IPC IPC(8): A24B15/20A24B15/26
CPCA24B15/20A24B15/26
Inventor 陈建华刘迎春黄春兴禤建国
Owner 昆明坤九香精香料有限公司
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