Unlock instant, AI-driven research and patent intelligence for your innovation.

A kind of preparation method of immobilized enzyme oil spill degradation agent

A technology of immobilized enzymes and degradants, applied in chemical instruments and methods, fixed on or in inorganic carriers, fixed on/in organic carriers, etc., can solve the problem of low repair efficiency, enzyme loss, enzyme Reduce activity and other problems, achieve the effect of high ball forming performance, high degradation rate and low cost

Active Publication Date: 2021-08-10
ZHEJIANG OCEAN UNIV
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, in the process of bioremediation of marine oil spills, there are problems such as serious enzyme loss, greatly reduced enzyme activity, and low repair efficiency.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of preparation method of immobilized enzyme oil spill degradation agent
  • A kind of preparation method of immobilized enzyme oil spill degradation agent
  • A kind of preparation method of immobilized enzyme oil spill degradation agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A preparation method of an immobilized enzyme oil spill degradation agent, comprising the following steps:

[0019] Adsorption: The sesame straw activated carbon and the enzyme liquid are evenly mixed according to the mass ratio of 1:5 for adsorption, the adsorption temperature is 23 ° C, and the constant temperature adsorption is 1.5 hours; the sesame straw activated carbon has a porous structure, and its mass transfer performance is strong, and it is compatible with the enzyme liquid It can produce a large area of ​​contact, which can greatly improve the activity and degradation efficiency of subsidiary microorganisms;

[0020] Crosslinking: Mix polyethylene glycol, sodium alginate, deionized water, and compound enzyme solution with a mass ratio of 1:4:9:15 to obtain a mixed solvent, and then add the solvent dropwise to the crosslinking agent at a weight ratio of 1:1.5 , control the temperature at 0°C, stir and cross-link in an ice-water bath for 5 minutes, then move ...

Embodiment 2

[0023] A method for preparing an immobilized enzyme oil spill degradation agent, comprising: adsorption and crosslinking, specifically comprising the following steps:

[0024] 1) Adsorption: The activated carbon from sesame straw and the enzyme liquid are evenly mixed according to the mass ratio of 1:6 for adsorption, the adsorption temperature is 25°C, and the constant temperature adsorption is 3.0 hours; The enzyme solution can produce a large area of ​​contact, which can greatly improve the activity and degradation efficiency of the attached microorganisms;

[0025] 2) Crosslinking: Mix polyethylene glycol, sodium alginate, deionized water, and compound enzyme solution with a mass ratio of 1:6:10:20 to obtain a mixed solvent. The molecular weight of polyethylene glycol is 800; sodium alginate The concentration of 1.05mol / L, the mass ratio of 1:0.3:2.0:15 methyl orthosilicate, 3,5-difluorobenzoic acid, methyltrioxymethylsilane and methanol were mixed in an ice-water bath to ...

Embodiment 3

[0031] A preparation method of an immobilized enzyme oil spill degradation agent, comprising the following steps:

[0032] Enzyme solution preparation: Suction filter the chlorella liquid after 10 days of cultivation and in the logarithmic growth phase. The filter membrane is 0.40 μm, remove the filter membrane containing chlorella, freeze it in liquid nitrogen, and then add 2.5 times of PBS buffer solution, the pH of PBS buffer solution is 7.2, and the concentration is 0.45mol / L; grind at 3°C, pour the grinding liquid into a centrifuge tube and centrifuge at 3°C, 11000r / min for 20 minutes, and take out the supernatant The obtained enzyme solution was stored at 2°C;

[0033] Phosphoric acid activation: Weigh the dried sesame straw powder and phosphoric acid and mix uniformly at a ratio of 1:1.5. After fully stirring, use a kneader to knead at 140°C for 45 minutes. After kneading, the sample is granulated into 4mm columnar particles. After the sample is hardened, Phosphoric ac...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
pore sizeaaaaaaaaaa
iodine adsorption valueaaaaaaaaaa
adsorption capacityaaaaaaaaaa
Login to View More

Abstract

The invention discloses a preparation method of an immobilized enzyme oil spill degradation agent, comprising: adsorption and cross-linking, uniformly mixing sesame straw activated carbon and enzyme liquid for constant temperature adsorption, adding polyethylene glycol, sodium alginate, deionized The mixture of water and compound enzyme liquid is added into the cross-linking agent, stirred and cross-linked to obtain the immobilized enzyme oil spill degradation agent. The beneficial effects are: the immobilized enzyme oil spill degradation agent has high mechanical strength, resistance to physical impact, high mass transfer performance and ball forming performance, can quickly and efficiently degrade oil, has no toxic and side effects, and has low cost. The process is simple and easy, suitable for large-scale production and application; the degradation rate of petroleum hydrocarbons can reach 70-90%, compared with free enzyme liquid, the degradation rate is increased by 35%-50%, which is a safe and efficient Immobilized enzyme oil spill degradation agent.

Description

technical field [0001] The invention relates to the field of environmental biology technology, in particular to a preparation method of an immobilized enzyme oil spill degradation agent. [0002] technical background [0003] At present, the bioremediation of oil pollution is mainly based on microbial remediation. Biological remediation is recognized worldwide as a new technology for efficiently remediating oil pollution. Using this technology to degrade and remove oil spills is the best way to restore the ecological environment. It is of great importance to protect my country's marine ecological environment and maintain the sustainable development of marine resources. scientific significance and application value. In microbial remediation technology, enzymatic degradation technology accounts for a large proportion. As a biodegradant, enzymes are more efficient than general chemical catalysts and are environmentally friendly. [0004] However, in the process of bioremediati...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/14C12N11/10C12N11/04C02F3/34C02F101/32C02F103/08
CPCC02F3/342C02F2101/32C02F2103/08C12N11/04C12N11/10C12N11/14
Inventor 陈庆国鲍博李晶晶刘梅穆军
Owner ZHEJIANG OCEAN UNIV