Red steppe cattle beef quality-related molecular marker and application thereof in meat quality identification
A molecular marker, beef quality technology, applied in the determination/inspection of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., can solve the problems of shortening the generation interval, polymorphism has not yet been reported, and difficulty in obtaining slaughter trait data, etc. Achieve the effect of great use value and social value
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Embodiment 1
[0031] This embodiment provides a molecular marker related to the quality of grassland red beef. The molecular markers related to the quality of grassland red beef include molecular markers of net meat rate of grassland red cattle, molecular markers of water loss rate of grassland red cattle, molecular markers of marbling of grassland red cattle, molecular markers of grassland red beef, and molecular markers of grassland red beef quality. Molecular markers of shear stress and pH molecular markers of Prairie Red Bull.
[0032] The molecular markers of lean meat rate of grassland red cattle include HNRNPA0 gene mutation site, PHACTR2 gene mutation site, RALYL gene mutation site and MYO18B gene mutation site, the water loss rate molecular markers of grassland red cattle include RIOK2 gene mutation site, Marbling molecular markers include CD109 gene mutation sites and CSMD1 gene mutation sites, grassland red cattle shear force molecular markers include LYST gene mutation sites, gra...
Embodiment 2
[0034] This embodiment provides the acquisition of molecular markers related to the quality of prairie red beef. The specific steps are as follows:
[0035] Sampling: Randomly select 48 heads of grassland red beef with a new strain, collect blood from the jugular vein, put the blood collected from the jugular vein into a centrifuge tube with EDTA anticoagulant, shake it well and quickly put it in an ice box, and store it at -80°C for later use;
[0036] Grassland red cattle carcass weight, net meat weight, on-site measurement and data acquisition of slaughter rate and net meat rate.
[0037] Slaughter the selected prairie red cattle, measure the carcass weight, net meat weight, and calculate the slaughter rate and net meat rate in accordance with the agricultural industry standard NY / T676-2003 "Beef Quality Classification" of the People's Republic of China;
[0038] 1.1 Carcass weight: refers to the weight without head, skin, tail, hooves, reproductive organs and surrounding f...
Embodiment 3
[0076] This embodiment provides the acquisition of a grassland red beef quality-related molecular marker, and the specific steps are as follows:
[0077] Sampling: Randomly select 48 heads of grassland red beef with a new strain, collect blood from the jugular vein, put the blood collected from the jugular vein into a centrifuge tube with EDTA anticoagulant, shake it well and quickly put it in an ice box, and store it at -80°C for later use;
[0078] Grassland red cattle carcass weight, net meat weight, on-site measurement and data acquisition of slaughter rate and net meat rate.
[0079] Slaughter the selected prairie red cattle, measure the carcass weight, net meat weight, and calculate the slaughter rate and net meat rate in accordance with the agricultural industry standard NY / T676-2003 "Beef Quality Classification" of the People's Republic of China;
[0080] 1.1 Carcass weight: refers to the weight without head, skin, tail, hooves, reproductive organs and surrounding fat,...
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