Red steppe cattle beef quality-related molecular marker and application thereof in meat quality identification

A molecular marker, beef quality technology, applied in the determination/inspection of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., can solve the problems of shortening the generation interval, polymorphism has not yet been reported, and difficulty in obtaining slaughter trait data, etc. Achieve the effect of great use value and social value

Pending Publication Date: 2018-03-20
JILIN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The new strain of Chinese prairie red beef was bred by the beef cattle research team of Jilin Provincial Academy of Agricultural Sciences Animal Husbandry Science Branch after years of selection The research on slaughter traits by breeding experts is not as extensive and in-depth as the research on growth traits, because it is relatively difficult to obtain data on these slaughter traits, especially for large livestock animals, which limits the slaughter traits to a certain extent. Research on genetic markers "However, as people's living standards continue to improve today, people's requirements for beef quality are also getting higher and higher, which requires beef cattle breeding experts to continuously strive to breed better beef cattle breeds to meet people's needs." In view of Considering the above considerations, domestic and foreign beef cattle breeding experts have used marker-assisted selection technology to intensify the research on genetic markers of beef cattle slaughter traits, thus laying the foundation for direct use of genotypes for early selection, shortening generation intervals, and accelerating genetic progress.
[0003] So far, there have been no reports at home and abroad on the polymorphism of the entire gene and its relationship with slaughter traits in the new line population of grassland red beef in China

Method used

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  • Red steppe cattle beef quality-related molecular marker and application thereof in meat quality identification
  • Red steppe cattle beef quality-related molecular marker and application thereof in meat quality identification
  • Red steppe cattle beef quality-related molecular marker and application thereof in meat quality identification

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Effect test

Embodiment 1

[0031] This embodiment provides a molecular marker related to the quality of grassland red beef. The molecular markers related to the quality of grassland red beef include molecular markers of net meat rate of grassland red cattle, molecular markers of water loss rate of grassland red cattle, molecular markers of marbling of grassland red cattle, molecular markers of grassland red beef, and molecular markers of grassland red beef quality. Molecular markers of shear stress and pH molecular markers of Prairie Red Bull.

[0032] The molecular markers of lean meat rate of grassland red cattle include HNRNPA0 gene mutation site, PHACTR2 gene mutation site, RALYL gene mutation site and MYO18B gene mutation site, the water loss rate molecular markers of grassland red cattle include RIOK2 gene mutation site, Marbling molecular markers include CD109 gene mutation sites and CSMD1 gene mutation sites, grassland red cattle shear force molecular markers include LYST gene mutation sites, gra...

Embodiment 2

[0034] This embodiment provides the acquisition of molecular markers related to the quality of prairie red beef. The specific steps are as follows:

[0035] Sampling: Randomly select 48 heads of grassland red beef with a new strain, collect blood from the jugular vein, put the blood collected from the jugular vein into a centrifuge tube with EDTA anticoagulant, shake it well and quickly put it in an ice box, and store it at -80°C for later use;

[0036] Grassland red cattle carcass weight, net meat weight, on-site measurement and data acquisition of slaughter rate and net meat rate.

[0037] Slaughter the selected prairie red cattle, measure the carcass weight, net meat weight, and calculate the slaughter rate and net meat rate in accordance with the agricultural industry standard NY / T676-2003 "Beef Quality Classification" of the People's Republic of China;

[0038] 1.1 Carcass weight: refers to the weight without head, skin, tail, hooves, reproductive organs and surrounding f...

Embodiment 3

[0076] This embodiment provides the acquisition of a grassland red beef quality-related molecular marker, and the specific steps are as follows:

[0077] Sampling: Randomly select 48 heads of grassland red beef with a new strain, collect blood from the jugular vein, put the blood collected from the jugular vein into a centrifuge tube with EDTA anticoagulant, shake it well and quickly put it in an ice box, and store it at -80°C for later use;

[0078] Grassland red cattle carcass weight, net meat weight, on-site measurement and data acquisition of slaughter rate and net meat rate.

[0079] Slaughter the selected prairie red cattle, measure the carcass weight, net meat weight, and calculate the slaughter rate and net meat rate in accordance with the agricultural industry standard NY / T676-2003 "Beef Quality Classification" of the People's Republic of China;

[0080] 1.1 Carcass weight: refers to the weight without head, skin, tail, hooves, reproductive organs and surrounding fat,...

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Abstract

The invention provides a red steppe cattle beef quality-related molecular marker and application thereof in meat quality identification and belongs to the fields of biotechnology and crop and pastureproduction. The red steppe cattle beef quality-related molecular marker provided by the invention is closely related to meat quality of red steppe cattle, and when the red steppe cattle beef quality-related molecular marker is applied to the meat quality identification of red steppe cattle, beef with higher quality can be better distinguished and screened out, so that the red steppe cattle beef quality-related molecular marker can better guide production of agriculture and animal husbandry, especially the animal husbandry, and has greater use value and social value.

Description

technical field [0001] The invention relates to the fields of biotechnology and agricultural and animal husbandry production, in particular to molecular markers related to grassland red beef quality and their application in meat quality identification. Background technique [0002] The new strain of Chinese prairie red beef was bred by the beef cattle research team of Jilin Provincial Academy of Agricultural Sciences Animal Husbandry Science Branch after years of selection The research on slaughter traits by breeding experts is not as extensive and in-depth as the research on growth traits, because it is relatively difficult to obtain data on these slaughter traits, especially for large livestock animals, which limits the slaughter traits to a certain extent. Research on genetic markers "However, as people's living standards continue to improve today, people's requirements for beef quality are also getting higher and higher, which requires beef cattle breeding experts to con...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 张国梁李旭秦立红郎洪彦武斌张莹赵玉民朱永超张鑫
Owner JILIN ACAD OF AGRI SCI
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