method for industrial cultivation of Pleurotus eryngii by using Pennisetum alopecuroides
A technology of Pennisetum and Pleurotus eryngii, which is applied in cultivation, plant cultivation, mushroom cultivation, etc., can solve the problems of increased production cost of cultivation raw materials, high cost, and easy mildew in the raw material storage process, so as to improve the biotransformation rate , the appropriate carbon-nitrogen ratio, and the effect of improving nutritional quality and flavor
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Embodiment 1
[0027] 1. Strain treatment
[0028] (1) Selection of mother species: Select the mother fungus with stable species, thick white mycelia, uniform and vigorous mycelium growth, and covered the inclined surface of the test tube;
[0029] (2) Inoculation and cultivation: The transfer of the PDA test tube is carried out in the ultra-clean workbench. Select a strain on the slant of the test tube and operate it next to the flame of the alcohol lamp. placed in an incubator for cultivation;
[0030] (3) Expansion of the original seed: the inoculation of the triangular flask is carried out in an ultra-clean workbench. The liquid stock culture medium contained in the triangular flask is: water 97.8%, soybean meal 0.29%, potassium dihydrogen carbonate 0.10%, magnesium sulfate 0.05%, glucose 1.47%, peptone 0.29%. Select a test tube slant strain, operate it next to the flame of an alcohol lamp, take double-layer newspapers to wrap the mouth of the bottle, stick a label on the triangular fl...
Embodiment 2
[0051] 1. Strain treatment
[0052] (1) Selection of mother species: Select the mother fungus with stable species, thick white mycelia, uniform and vigorous mycelium growth, and covered the inclined surface of the test tube;
[0053] (2) Inoculation and cultivation: The transfer of the PDA test tube is carried out in the ultra-clean workbench. Select a strain on the slant of the test tube and operate it next to the flame of the alcohol lamp. put into the incubator for cultivation;
[0054] (3) Expansion of the original seed: the inoculation of the triangular flask is carried out in an ultra-clean workbench. The liquid stock culture medium contained in the triangular flask is: water 97.8%, soybean meal 0.29%, potassium dihydrogen carbonate 0.10%, magnesium sulfate 0.05%, glucose 1.47%, peptone 0.29%. Choose a test tube slant strain, operate it next to the flame of an alcohol lamp, take double-layer newspapers to wrap the mouth of the bottle, stick a label on the triangular fl...
Embodiment 3
[0075] 1. Strain treatment
[0076] (1) Selection of mother species: Select a mother fungus with stable species, thick white mycelia, uniform and vigorous mycelium growth, and covered the inclined surface of the test tube;
[0077] (2) Inoculation culture: The transfer of the PDA test tube is carried out in the ultra-clean workbench. Select a test tube slant strain and operate it next to the flame of the alcohol lamp. put into the incubator for cultivation;
[0078] (3) Expansion of the original seed: the inoculation of the triangular flask is carried out in an ultra-clean workbench. The liquid stock culture medium contained in the triangular flask is: water 97.8%, soybean meal 0.29%, potassium dihydrogen carbonate 0.10%, magnesium sulfate 0.05%, glucose 1.47%, peptone 0.29%. Select a test tube slant strain, operate it next to the flame of an alcohol lamp, take double-layer newspapers to wrap the mouth of the bottle, stick a label on the triangular flask, and put it into a sha...
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