A Double Dominant Marker for Accurate Identification of Onion S/N Cytoplasmic Genotype and Its Application

Abstract

The invention discloses a double-dominant marker for accurately authenticating an S / N cytoplasm genotype of onions and application of the double-dominant marker. The length of a specific fragment of an S-form cytoplasm in the double-dominant marker is 232bp, and the length of a specific fragment of an N-form cytoplasm is 196bp. The invention further discloses SCAR marking primers SEQ ID NO.5 and SEQ ID NO.6. A prepared SCAR molecular marker is utilized for rapidly and accurately judging cytoplasm types of onion materials, so that the misclassification of cytoplasm genotypes caused due to experimental operate miss and invalid PCR amplification can be avoided; and furthermore, DNA can be extracted from mixed group samples when a maintainer line is selected in a large group and can be used for primarily screening whether a maintainer line single plant exists, so that the selection efficiency is greatly improved, the time and the cost are saved, and the double-dominant marker has importantsignificances to the matching of a breeding sterile line and a maintainer line of the onions and the breeding of hybrid varieties of the onions.

Description

Technical field [0001] The invention belongs to the technical field of plant breeding, and relates to a molecular marker for onion breeding and its application, in particular to a dual dominant molecular marker for identifying onion S / N cytoplasmic genotype and its application, and can be applied to molecular marker assisted breeding. Accurate selection of onion male sterile lines and supporting maintainers. Background technique [0002] Onion (Allium cepa.L), also known as ball onion, round onion, jade onion, green onion, Dutch onion, scallion, etc., is a two-year-old diploid plant belonging to the Allium genus of the Liliaceae. It has been cultivated for more than 5000 years. Onions were introduced to my country in modern times, and they have only been cultivated in my country for more than 100 years. However, due to their strong adaptability and resistance to storage and transportation, although onions have a short cultivation history in my country, they have developed very fas...

AI Technical Summary

Problems solved by technology

Although this marker can effectively avoid misclassification of cytoplasmic types due to experimental manipulation errors and ineffective PCR amplification, due to the lack of stable N-type cytoplasmic specific markers, it is impossible to select maintainer lines from large populations. Extract DNA from mixed population samples for preliminary screening of maintainer individual plants, which can only be verified by individual plants, which affects the selection efficiency

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