A kind of preparation method of immobilized microalgae

A technology of microalgae and microalgae culture solution, applied in the direction of microorganism-based methods, biochemical equipment and methods, fixed on/in organic carriers, etc., can solve the difficult and difficult treatment of microalgae cell biomass in sewage Poor effect stability, secondary pollution treatment water and other problems, to achieve the effect of less loss of microalgae cells, easy separation, and fast reaction speed

Active Publication Date: 2020-05-19
安徽双淮环境科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, because microalgae cells are generally smaller than 30 μm, negatively charged, and have a density close to that of water, these characteristics make algae cells in a stable suspension state in water, resulting in a large loss of microalgae cells with the treated water, which will not only cause secondary pollution Treating water, and making it difficult to maintain a large amount of microalgae cell biomass in treated sewage (generally only 0.2-0.6g / L)
Low culture density leads to low removal efficiency, making the treatment effect less stable

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The present invention proposes a preparation method of immobilized microalgae, comprising the following steps:

[0025] S1. Under aseptic conditions, chlorella vulgaris was mixed with 5.4×10 6 The inoculation amount of each / mL was inoculated into the culture medium. Under the conditions of temperature of 26°C and rotation speed of 122 rpm, the light and dark cycles were alternately cultivated. The culture was carried out in the light environment for 9 hours, and the light intensity was 3700 Lux. The time of under-cultivation is 11 hours, and after 68 hours of alternate cultivation in a light environment and a dark environment, a microalgae culture solution is obtained;

[0026] S2, adjusting the pH value of the microalgae culture solution obtained in S1 to 5.2, adding 1.58% sodium citrate of the microalgae culture solution weight, stirring for 25 minutes, adding 4.1% calcium chloride of the microalgae culture solution weight, Stir for 44 minutes, and centrifuge at 2100...

Embodiment 2

[0030] The present invention proposes a preparation method of immobilized microalgae, comprising the following steps:

[0031] S1. Under aseptic conditions, the common Chlorella was mixed with 1.6×10 6 The inoculum amount per mL was inoculated into the culture medium. Under the condition of temperature of 25°C and rotation speed of 128 r / min, alternating light and dark cycle culture was carried out. The culture was carried out in the light environment for 10 hours, and the light intensity was 3600 Lux. The time of under-cultivation is 12 hours, and after 73 hours of alternate cultivation in light environment and dark environment, the microalgae culture solution is obtained;

[0032] S2, adjusting the pH value of the microalgae culture solution obtained in S1 to 5.3, adding 1.67% sodium citrate of the microalgae culture solution weight, stirring for 22 minutes, adding 3.9% calcium chloride of the microalgae culture solution weight, Stir for 48 minutes, and centrifuge at 2200 r...

Embodiment 3

[0036] The present invention proposes a preparation method of immobilized microalgae, comprising the following steps:

[0037] S1. Under aseptic conditions, chlorella vulgaris was mixed with 8.9×10 6 The inoculum amount per mL was inoculated into the culture medium, and under the conditions of temperature of 27°C and rotation speed of 113 rpm, alternating light and dark cycle culture was carried out, and the culture was carried out in the light environment for 8 hours, and the light intensity was 3800 Lux. The time of under-cultivation is 13 hours, and after 62 hours of alternate cultivation in a light environment and a dark environment, a microalgae culture solution is obtained;

[0038] S2, adjusting the pH value of the microalgae culture solution obtained in S1 to 5.1, adding 1.51% sodium citrate of the microalgae culture solution weight, stirring for 28 minutes, adding 4.2% calcium chloride of the microalgae culture solution weight, Stir for 41 minutes, and centrifuge at ...

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Abstract

The invention discloses a preparation method of immobilized microalgae. The preparation method comprises the following steps: under sterile operation conditions, inoculating chlorella into a culture medium, carrying out illumination and dark cycle alternation cultivation for 60 to 75 h to obtain a microalgae culture solution; adjusting the pH value of the microalgae culture solution to be 4.5 to 6, adding sodium citrate, stirring for 20 to 30 min, adding calcium chloride, stirring for 40 to 50 min, and carrying out centrifugal separation to obtain a microalgae solution; preparing a carrier solution containing sodium alga acid and magnesium chloride, adding the microalgae solution into the carrier solution, after uniform mixing, dropwise adding the mixture into a calcium chloride solution with the mass fraction of 2.6% to 3.2% by an injection syringe, taking out microalgae after being immobilized for 2 to 2.5 h at the temperature of 8 to 10 DEG C, and desalting the immobilized microalgae for 1 to 2 h with deionized water. The immobilized microalgae prepared by adopting the method has the advantages that the treatment effect is stable and secondary pollution can be effectively prevented.

Description

technical field [0001] The invention relates to the field of preparation of microalgae sewage treatment agent, in particular to a preparation method of immobilized microalgae. Background technique [0002] With the large-scale development of my country's livestock and poultry breeding industry, nearly one billion tons of livestock and poultry breeding manure will be produced every year, which poses a serious threat to limited water resources. [0003] At present, there are two main treatment methods for aquaculture sewage: one is to use biogas digesters for anaerobic fermentation, but the huge amount of biogas slurry still contains high concentrations of nutrients such as nitrogen and phosphorus. The arable land for absorbing biogas slurry is becoming increasingly scarce, and the resulting biogas slurry is directly discharged into the water body, which will lead to severe eutrophication of the natural water body; the second is biological removal, and biological treatment use...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): C12N11/10C12N11/04C12R1/89
CPCC12N11/04C12N11/10
Inventor周燕王建泉周洁
Owner安徽双淮环境科技有限公司