A rapid detection method and kit for dicofol residues

Abstract

The invention discloses a dicofol residue rapid detection method and a kit thereof. The dicofol residue rapid detection method comprises the following steps: extracting a sample, extracting a solid phase, after extracting a sample extract through a solid-phase extraction column, eluting the sample extract by an eluate, adding 2,2-dipyridine, a strong alkaline solution and tetramethylammonium hydroxide in the eluted eluate, and performing a chromogenic reaction and colorimetric determination to obtain the dicofol amount. According to the invention, sample detection can be completed by only 20 min, dipyridine having the characteristics of odorlessness and safety is taken as a reaction developer, and an experiment method realizes green environmental protection; tetramethylammonium hydroxide is added as a reaction catalyst, so that the chromogenic reaction can be completed in two minutes, and the chromogenic effect is enhanced; the method determines the dicofol content by comparing the colors, sensitivity is high, and the method can be widely used for rapidly detecting dicofol in samples such as vegetables, fruits, and tea.

Description

technical field [0001] The invention relates to a detection method and kit for pesticide residues, in particular to a color reaction-based rapid detection method for dicofol and a kit. Background technique [0002] Dicofol is a broad-spectrum and low-toxicity organochlorine acaricide, which is mainly used to prevent and control a variety of acarids in cotton, fruit trees, flowers, etc. Since dicofol is not easy to decompose and has a high residue, it is not suitable for use in tea, vegetables and edible fungi. However, the use of dicofol in my country is still relatively common at present, and the detection rate of dicofol in tea, vegetables and edible fungi is relatively high. [0003] Dicofol will undergo alkaline hydrolysis under alkaline conditions to produce chloroform and other substances. Chloroform will react in the pyridine-NaOH solution system to form a red compound, and the concentration of dicofol can be calculated according to the absorbance value of the red c...

AI Technical Summary

Problems solved by technology

Most of the existing rapid detection methods for dicofol use the pyridine-alkaline colorimetric method, and pyridine is a volatile and odorous toxic solvent, which will cause certain harm to the human body

At the same time, the accuracy of this method is not high, the operation time is long, and its application is limited

[0004] The reactivity of bipyridine is far weaker than that of pyridine, and it basically does not react with chloroform to develop color. There is no use of bipyridine to detect dicofol in the prior art

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