Application of Apios americana Medikus leaf polysaccharide to reduction of hepatocyte lipid deposition

A technology of lipid deposition and liver cells, which is applied in the field of medicine, can solve the problems of easy rebound and lower immunity of the body, and achieve the effect of reducing cell lipid deposition, good development prospects, and expanding application fields

Active Publication Date: 2018-04-24
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Most of the other weight loss and fat reduction methods also have the disadvantages of lowering the body's immunity and easy rebound.

Method used

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  • Application of Apios americana Medikus leaf polysaccharide to reduction of hepatocyte lipid deposition
  • Application of Apios americana Medikus leaf polysaccharide to reduction of hepatocyte lipid deposition
  • Application of Apios americana Medikus leaf polysaccharide to reduction of hepatocyte lipid deposition

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Embodiment 1

[0064] Embodiment 1, a kind of preparation method of bean taro leaf polysaccharide, carries out following steps successively:

[0065] 1), the preparation of the crude product of bean taro leaf polysaccharide:

[0066] According to the solid-liquid ratio of 1g:10ml, weigh 100g of taro leaf and add 1000mL distilled water, extract in 80°C water bath for 4h (enzyme inactivation is also achieved during extraction), then beat, and then centrifuge (4000r / min for 30min), and Suction filter the supernatant obtained by centrifugation and collect the filtrate;

[0067] Replace the taro leaf with the filter residue obtained by centrifugation, repeat the above process of adding distilled water, then extracting (4h in 80°C water bath), beating, and centrifuging twice, and combine the filtrate obtained by centrifugation and suction for three times and concentrate to the original volume by rotary evaporation 1 / 5 of the concentrated solution.

[0068] Under stirring conditions, add ethanol ...

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Abstract

The invention discloses application of Apios americana Medikus leaf polysaccharide to reduction of hepatocyte lipid deposition. The Apios americana Medikus leaf polysaccharide is used for preparing medicines and health-care foods, which are used for treating or preventing nonalcoholic fatty liver. A preparation method of the Apios americana Medikus leaf polysaccharide comprises the following steps: 1) preparing an Apios americana Medikus leaf polysaccharide crude product: after mixing Apios americana Medikus leaves and distilled water, extracting; then pulping, centrifuging and concentrating;adding ethanol into an obtained concentrated solution until the final concentration of the ethanol is 85+ / -5 percent; standing at 4+ / -1 DEG C for 24+ / -2h; then collecting sediment to obtain Apios americana Medikus leaf crude polysaccharide; 2) purifying the Apios americana Medikus leaf crude polysaccharide, including carrying out activated carbon decoloration and Sevage-method protein removal on the Apios americana Medikus leaf crude polysaccharide.

Description

technical field [0001] The invention relates to the field of medicine, in particular to the preparation of bean taro leaf polysaccharide and its application in the preparation of health food and medicine for the prevention and treatment of non-alcoholic fatty liver; at the same time, it relates to reducing the lipid deposition of HepG2 cells induced by oleic acid in the prevention and treatment of non-alcoholic fatty liver. application in fatty liver. Background technique [0002] Apios americana Medikus is a perennial leguminous plant (Papilionaceae subfamily) native to the eastern part of North America from Canada to the southern part of Florida. Its edible part is the underground tuber, and its protein content is higher than that of other plant tubers. It is a crop with both nutrition and economic value, so it is often used as a staple food by Indians. Bean taro has been successfully introduced in China since 2009, and has been gradually promoted and planted in Fuyang, J...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/715A61P1/16A23L33/105
CPCA23V2002/00A61K31/715A23L33/105A23V2250/21A23V2200/32
Inventor 郑晓冬楚强俞露霜刘阳阳李永璐
Owner ZHEJIANG UNIV
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