The application of taro leaf polysaccharide in reducing lipid deposition in liver cells

A lipid deposition and hepatocyte technology, applied in the field of medicine, can solve the problems of reducing body immunity, easy to rebound, etc., achieves good development prospects, reduces cell lipid deposition, and expands the application field.

Active Publication Date: 2021-03-02
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the other weight loss and fat reduction methods also have the disadvantages of lowering the body's immunity and easy rebound.

Method used

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  • The application of taro leaf polysaccharide in reducing lipid deposition in liver cells
  • The application of taro leaf polysaccharide in reducing lipid deposition in liver cells
  • The application of taro leaf polysaccharide in reducing lipid deposition in liver cells

Examples

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Comparison scheme
Effect test

Embodiment 1

[0064] Embodiment 1, a kind of preparation method of bean taro leaf polysaccharide, carries out following steps successively:

[0065] 1), the preparation of the crude product of bean taro leaf polysaccharide:

[0066] According to the solid-liquid ratio of 1g:10ml, weigh 100g of taro leaf and add 1000mL distilled water, extract in 80°C water bath for 4h (enzyme inactivation is also achieved during extraction), then beat, and then centrifuge (4000r / min for 30min), and Suction filter the supernatant obtained by centrifugation and collect the filtrate;

[0067] Replace the taro leaf with the filter residue obtained by centrifugation, repeat the above process of adding distilled water, then extracting (4h in 80°C water bath), beating, and centrifuging twice, and combine the filtrate obtained by centrifugation and suction for three times and concentrate to the original volume by rotary evaporation 1 / 5 of the concentrated solution.

[0068] Under stirring conditions, add ethanol ...

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Abstract

The invention discloses an application of bean taro leaf polysaccharide in reducing lipid deposition in liver cells; it is used for preparing medicine and health food for treating or preventing non-alcoholic fatty liver. The preparation method of the bean taro leaf polysaccharide is as follows: 1), the preparation of the crude product of the bean taro leaf polysaccharide: first extract the bean taro leaf and distilled water, then beat, centrifuge, and concentrate; add ethanol to the obtained concentrated solution The final concentration is 85±5%, and after standing at 4±1°C for 24±2 hours, the precipitate is collected to obtain the crude polysaccharide from bean and taro leaves; 2), the purification of the crude polysaccharide from bean and taro leaves includes decolorizing the crude polysaccharides from bean and taro leaves with activated carbon and Sevage deproteinization.

Description

technical field [0001] The invention relates to the field of medicine, in particular to the preparation of bean taro leaf polysaccharide and its application in the preparation of health food and medicine for the prevention and treatment of non-alcoholic fatty liver; at the same time, it relates to reducing the lipid deposition of HepG2 cells induced by oleic acid in the prevention and treatment of non-alcoholic fatty liver. application in fatty liver. Background technique [0002] Apios americana Medikus is a perennial leguminous plant (Papilionaceae subfamily) native to the eastern part of North America from Canada to the southern part of Florida. Its edible part is the underground tuber, and its protein content is higher than that of other plant tubers. It is a crop with both nutrition and economic value, so it is often used as a staple food by Indians. Bean taro has been successfully introduced in China since 2009, and has been gradually promoted and planted in Fuyang, J...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/715A61P1/16A23L33/105
CPCA23V2002/00A61K31/715A23L33/105A23V2250/21A23V2200/32
Inventor 郑晓冬楚强俞露霜刘阳阳李永璐
Owner ZHEJIANG UNIV
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