Large field of view super-resolution dynamic phase lensless microscopic imaging device and reconstruction method

Abstract

The invention discloses a large-field-of-view super-resolution dynamic phase non-lens micro-imaging device and a reconstruction method. The device includes a partially coherent or coherent light source, parallel flat plates, a sample table and a camera which are arranged in sequence to constitute an imaging system. The partially coherent or coherent light source is placed at an uppermost plate ofthe entire imaging system, and the position of the light emission center of the light source is located on an optical axis of the entire imaging system. The device can achieve large stepping and smalldisplacement without any precise sub-pixel displacement device, and therefore the system structure is simplified, the microscope size is reduced, and the cost is greatly reduced; according to the method, the requirement on the mechanical structure accuracy is reduced, great resistance capability to noise in acquired images is achieved, and a large-field-of-view super-resolution phase image can bereconstructed very steadily and accurately.

Description

technical field [0001] The invention belongs to lensless optical microscopic imaging technology, in particular to a lensless microscopic imaging device and a reconstruction method for super-resolution dynamic phase of a large field of view. Background technique [0002] In the field of microscopic imaging, high resolution has always been the pursuit of the goal, but traditional microscopes often sacrifice the imaging field of view to obtain higher imaging resolution, that is, the spatial bandwidth product of the microscope does not increase together with the improvement of high resolution. Because the spatial resolution and the field of view are a pair of contradictions that are difficult to reconcile, in short, the whole picture of the object under inspection can be seen under a low-power lens, but when it is replaced with a high-power objective lens, only a small part of the object under inspection can be seen. small portion. At present, in order to break through the cont...

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Problems solved by technology

However, the above methods all need to rely on precise mechanical movement, such as 1 arc-second precise rotary translation stage, sub-pixel lateral translation stage, etc., or adjustable multi-wavelength light source (need to rely on high-precision wavelength tunable laser), these mechanical structure significantly increases system cost and reduces robustness

In addition, for lensless microscopy imaging, most of the existing super-resolution methods are limited to intensity super-resolution, which is difficult to meet the current needs of non-invasive imaging in the biomedical field, that is, in the field of bioimaging, biological samples stained Labeling by other means will affect the activity of the sample, but for most unstained biological cell samples, due to their weak absorption in the visible light band, they cannot be directly collected by the camera, so phase information must be used imaging

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