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A kind of denitrification microorganism and its application

A seed and ammonia nitrogen technology, applied in the direction of microorganisms, microorganisms, and methods based on microorganisms, can solve the problems of the late start of research on heterotrophic nitrifying bacteria

Active Publication Date: 2021-04-23
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the research on heterotrophic nitrifying bacteria started late, and due to the complex factors in the real environment and many problems in practical application, its technological process is still in the stage of laboratory experiments, and a large amount of research is needed to realize its industrial application as soon as possible. Applications

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  • A kind of denitrification microorganism and its application
  • A kind of denitrification microorganism and its application

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Embodiment 1

[0016] Example 1: Screening of denitrifying microorganisms (1) strain source: tannery wastewater (2) enrichment culture method: put 5mL water sample taken into a medium containing 100mL (0.5g / L (NH4)2SO4) In a 250ml Erlenmeyer flask (sterilized at 121°C for 20 minutes), enrich the culture at 30°C and 150rpm / min for 1-2 days, take 1mL of the enriched culture solution into a new (NH4)2SO4 concentration of 1g / L In the culture medium, keep the culture conditions unchanged, culture for 1-2 days, repeat the above steps until the concentration of (NH4)2SO4 in the culture medium is 2.5g / L Spread it on a plate containing 2.5g / L (NH4)2SO4, culture for 1-2d, and select a single colony for the next step of screening. Pick 200 pure colonies with good growth from the plate, inoculate them into the medium containing 0.5g / L (NH4)2SO4, shake the flask for 24 hours, and measure the degradation rate of ammonia nitrogen. The strain with the highest denitrification rate was identified by 16S rDNA...

Embodiment 2

[0017] Embodiment 2: Klebsiella oxytoca (Klebsiella oxytoca) TN-10 degrades ammonia nitrogen in waste water (1) Preparation of seed liquid: get the bacterial strain glycerol tube stock solution that preserves at-80 ℃ with 1% (V / V) The inoculation amount was inoculated into the seed medium, placed on a shaker at 30°C, and cultured with shaking at 150rpm for 24 hours; (2) Fermentation experiment: Take 1mL seed liquid and inoculate into 100mL (0.5g / L (NH 4 ) 2 SO 4 ) culture medium in a 250ml Erlenmeyer flask (sterilized at 121°C for 20min), in a shaker at 30°C, 150rpm / min shaking culture for 24h; (3) Determination of ammonia nitrogen content: after 24h fermentation, measure the ammonia nitrogen in the fermentation medium content. According to the ammonia nitrogen degradation rate formula, the degradation rate is 96.45% ( figure 1 ).

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Abstract

The present invention discloses a microorganism that can be used to efficiently remove ammonia nitrogen in leather wastewater, named as Klebsiella oxytoca ( Klebsiella oxytoca ) TN‑10, which was deposited in the China Center for Type Culture Collection on April 14, 2017, at Wuhan, China, with the deposit number CCTCC NO: M2017193. This bacterial strain is screened from leather wastewater, and the Klebsiella oxytocin found in the present invention ( Klebsiella oxytoca ) TN‑10 can efficiently and quickly degrade ammonia nitrogen in leather wastewater, purify water quality, and reduce the harm of nitrogen-containing pollutants to aquatic organisms in the environment, and has broad application prospects.

Description

technical field [0001] The invention belongs to the field of environmental microorganisms and applications thereof. Specifically, it relates to a strain of Klebsiella oxytoca and the application of the strain in the degradation of ammonia nitrogen in wastewater. Background technique [0002] With the development of industry and the improvement of people's living standards, the nitrogen content in urban sewage and industrial wastewater has risen sharply. The nitrogen content in receiving water bodies such as rivers and lakes has far exceeded the self-purification capacity of the water body, causing water pollution, causing excessive groundwater substance content and eutrophication of surface water. Algae can proliferate in large numbers in water bodies with high nitrogen content, which will reduce the dissolved oxygen content in the water body and cause a large number of fish deaths. After the death of algae, anaerobic fermentation in the water body will make the water body ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C02F101/16C12R1/22
CPCC02F3/34C02F2101/16C12N1/205C12R2001/22
Inventor 吴重德李丹周荣清黄钧
Owner SICHUAN UNIV