Modification method and application of neospora caninum surface protein SRS2 gene

A technology of Neospora caninum and surface protein, applied in the field of genetic engineering

Inactive Publication Date: 2018-06-08
CHONGQING AULEON BIOLOGICALS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, effective vaccines and therapeutic drugs have not been developed for the prevention and control of this disease. Accurate detecti

Method used

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  • Modification method and application of neospora caninum surface protein SRS2 gene
  • Modification method and application of neospora caninum surface protein SRS2 gene
  • Modification method and application of neospora caninum surface protein SRS2 gene

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Experimental program
Comparison scheme
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Embodiment Construction

[0014] The specific scheme is described below in conjunction with the examples.

[0015] 1. Materials

[0016] 1) Materials:

[0017] ①Plasmid mini-extraction kit and microgel recovery kit were purchased from Beijing Kangwei Century Biotechnology Co., Ltd.

[0018] ② Agarose agarose was purchased from Beijing Dingguo Changsheng Biotechnology Co., Ltd.

[0019] ③ Restriction enzymes EcoR V, Sac I, T4 DNA ligase, Trans5α, Transetta competent cells, etc. were purchased from Beijing Quanshijin Biotechnology Co., Ltd.

[0020] ④PET 32a was purchased from BioVector Plasmid Vector Strain Cell Protein Antibody Gene Collection Center.

[0021] ⑤ Amp was purchased from Sigma, USA.

[0022] ⑥IPTG was purchased from Chengdu Aoke Biological Co., Ltd.

[0023] 2. Introduction of the method

[0024] 1) Genetic modification:

[0025] ①Original SRS2 gene acquisition method: Obtained from the Neospora caninum surface protein (SRS2) gene (ID: JQ410454.1) registered in GenBank.

[0026] ②...

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Abstract

The invention relates to a modification method and an application of a neospora caninum surface protein SRS2 gene and belongs to the technical field of genetic engineering. On the basis of complete SRS2 genes, 168 basic groups are truncated from an N-terminal, 75 basic groups are truncated from a C-terminal, the truncated tSRS2 gene has the sequence of SEQ ID No.1, a target gene E.Coli codon optimization and inducible expression and purification of target protein are completed, and a kit is used for clinically detecting neosporiasis.

Description

technical field [0001] The invention relates to a modification method and application of the Neospora caninum surface protein SRS2 gene and belongs to the technical field of genetic engineering. Background technique [0002] Canine Neosporidiosis is a protozoan disease that is parasitic by Neospora caninum in the nucleated cells of various mammals. The disease can cause abortion of pregnant dams, stillbirth or neonatal movement disorders and neurological diseases, etc. The abortion caused by it has brought huge economic losses to farmers. The mode of transmission of the disease includes horizontal transmission and vertical transmission, and vertical transmission is its main mode of transmission. Among them, pregnant cattle are particularly sensitive to the disease. At present, effective vaccines and therapeutic drugs have not been developed for the prevention and control of this disease. Accurate detection and monitoring methods combined with decisive "elimination of infec...

Claims

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Application Information

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IPC IPC(8): C12N15/30C12N15/70C12N1/21C07K14/44C12R1/19
CPCC07K14/44C12N15/70C12N2800/22
Inventor 曹政马力克·艾则孜付利芝赵扬扬王凤莲吕航
Owner CHONGQING AULEON BIOLOGICALS
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