Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Rodent model of prostate cancer

A technology for rodents and prostates, applied in animal cells, vertebrate cells, microorganisms, etc., can solve problems such as limiting correlations

Inactive Publication Date: 2018-06-08
REGENERON PHARM INC
View PDF12 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Furthermore, the prevalence of neuroendocrine carcinomas in some mouse models also limits the relevance of these models to small subpopulations of patients with neuroendocrine disease

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Rodent model of prostate cancer
  • Rodent model of prostate cancer
  • Rodent model of prostate cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0125] Example 1. Production of Luci-TRAMP mice

[0126] This example illustrates the generation of transgenic mice (also referred to herein as Luci-TRAMP mice) comprising a transgene comprising the nucleic acid coding sequence for the SV40 tumor antigen-T2A-luciferase polypeptide flanked by The 5' regulatory region and the 3' regulatory region of the mouse probasin gene.

[0127] through bacterial homologous recombination and technology to construct vectors containing transgenes (see for example U.S. 6,586,251 and Valenzuela et al., High-throughput engineering of the mousegenome coupled with high-resolution expression analysis, 2003, Nature Biotech. 21(6):652-659). Briefly, RP23-417B18 was cloned using a bacterial artificial chromosome (BAC) containing the mouse probasin gene and modified as follows. A fragment consisting of 75 base pairs (bp) of mouse 5'-UTR, SV40-T antigen (without stop codon), followed by T2A sequence, NcoI / XhoI / NheI restriction site and stop codon and ...

Embodiment 2

[0132] Example 2. Longitudinal analysis of the Luci-TRAMP cohort

[0133] Luci-TRAMP mice generated from ES clone A-C10(5x) were designated MAID 2319 and used in the experiments described in this example. A mouse named MAID 2363 containing the transgene "R26-L-luc2" at the Rosa 26 locus in the genome (which expresses a luciferase reporter in a non-tissue-specific manner) and wild-type mouse F1H4 were used as controls .

[0134] Cohorts of 35 MAID2319 mice (Luci-TRAMP) were bred to allow prostate tumor development, while an equal number of MAID 2363 mice (R26-L-Luc2) and F1H4 mice were bred. Five mice were removed from each group at 2, 4, 6, 8, 11 and 16 months for in vivo luciferase imaging, μCT scanning (to assess lean muscle / fat / bone mass), histopathology Physiological analysis (starting at 4 months) and molecular phenotyping (RNA-seq and qPCR).

[0135] For longitudinal studies, at each indicated time point (inset below), fluorescein (150 mg / kg PerkinElmer) was administe...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

This disclosure provides a rodent model of prostate cancer. The rodents disclosed herein comprise a transgene that provides prostate-specific expression of an oncogenic protein (e.g, an SV40 tumor antigen) under the control of 5' and 3' regulatory regions of a mouse probasin gene. The rodents develop progressive forms of prostate tumor that resemble the development of human prostate cancer.

Description

[0001] Cross References to Related Applications [0002] This application claims the benefit of priority to US Provisional Application No. 62 / 213,389, filed September 2, 2015, the entire contents of which are incorporated herein by reference. field of invention [0003] The present disclosure relates to a rodent model of prostate cancer. The rodents disclosed herein comprise a transgene comprising the SV40 tumor antigen coding sequence operably linked to the 5' and 3' regulatory regions of the mouse probasin gene. The rodents disclosed herein exhibit prostate-specific expression of the transgene and develop progressive forms of prostate tumors that mimic the development of human prostate cancer. [0004] The sequence listing is incorporated by reference [0005] The sequence listing is an ASCII text file named 32350_10161US01_SequenceListing.txt with a size of 440KB created on August 24, 2016, submitted to the US Patent and Trademark Office via EFS-Web, and incorporated here...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/027C12N15/85
CPCA01K67/0275A01K2217/05A01K2227/105A01K2267/0331A01K2267/0393C07K2319/50C07K2319/61C12N2015/8572C12N2830/008C12N2830/36A01K67/0271A01K2207/12A01K2217/052C07K7/08C12N5/0606
Inventor C-J·邵H-C·李Z·李K-M·V·赖G·瑟斯顿
Owner REGENERON PHARM INC
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com