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Reagent for detecting urine creatinine and application of reagent

A technology of reagents and creatinine, which is applied in the field of medical testing, can solve the problems of poor precision and accuracy of test results, and cannot give the content, etc., achieve high accuracy and precision, improve precision and accuracy, and have a good linear relationship Effect

Active Publication Date: 2018-06-12
吉林省汇酉生物技术股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, most of the test strips for detecting urine creatinine on the market are semi-quantitative detection methods, which can only give an approximate content range, but cannot give an accurate content
In addition, the precision and accuracy of the test results are likely to be poor due to inappropriate reagent selection

Method used

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  • Reagent for detecting urine creatinine and application of reagent
  • Reagent for detecting urine creatinine and application of reagent
  • Reagent for detecting urine creatinine and application of reagent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1: reagent of the present invention and test strip

[0037] 1. Reagent composition

[0038] Liquid A is: sodium citrate 0.1mol / L, Tris 0.2mol / L, pH range 7.0, phytic acid 0.3% (w / v), copper sulfate 0.1% (w / v), EDTA 50mmol / L, sulfonphthalein Sodium 1.0% (w / v);

[0039] Solution B is: TMB 0.3% (w / v), PVP 5% (w / v), cumene hydroperoxide 5% (w / v), dissolved in ethanol.

[0040] 2. Preparation of test strips

[0041] Soak the filter paper in liquid A evenly, absorb the excess solution, and dry in a drying oven at a drying temperature of 90°C-105°C and a drying time of 60-70min;

[0042] Soak the dried filter paper soaked in liquid A in liquid B, absorb the excess solution, and dry in a drying oven at a drying temperature of 70°C-90°C and a drying time of 50-60min.

[0043] The dried filter paper is cut into strips, pasted on the PVC sheet, and then cut into small strips. The size of the test paper block is 5mm×5mm.

Embodiment 2

[0044]Embodiment 2: reagent of the present invention and test strip

[0045] 1. Reagent composition

[0046] Liquid A is: sodium citrate 0.5mol / L, Tris 0.1mol / L, pH range 6.0, phytic acid 0.1% (w / v), copper sulfate 0.3% (w / v), EDTA 50mmol / L, sulfonphthalein Sodium 0.1% (w / v);

[0047] Solution B is: TMB 1% (w / v), PVP 1% (w / v), 2,5-dimethyl-2,5-bis(tert-butylperoxy)hexane 5% (w / v ), dissolved in ethanol.

[0048] 2. Preparation of test strips

[0049] Soak the filter paper in liquid A evenly, absorb the excess solution, and dry in a drying oven at a drying temperature of 90°C-105°C and a drying time of 60-70min;

[0050] Soak the dried filter paper soaked in liquid A in liquid B, absorb the excess solution, and dry in a drying oven at a drying temperature of 70°C-90°C and a drying time of 50-60min.

[0051] The dried filter paper is cut into strips, pasted on the PVC sheet, and then cut into small strips. The size of the test paper block is 5mm×5mm.

Embodiment 3

[0052] Embodiment 3: reagent of the present invention and test strip

[0053] 1. Reagent composition

[0054] Liquid A is: sodium citrate 0.25mol / L, Tris 0.25mol / L, pH range 6.5, phytic acid 0.5% (w / v), copper sulfate 0.2% (w / v), EDTA 50mmol / L, sulfonphthalein Sodium 0.5% (w / v);

[0055] Solution B is: TMB 0.5% (w / v), PVP 3% (w / v), cumene hydroperoxide 2% (w / v), 2,5-dimethyl-2,5-bis(tert Butyl peroxy) hexane 3% (w / v), dissolved in methanol.

[0056] 2. Preparation of test strips

[0057] Soak the filter paper in liquid A evenly, absorb the excess solution, and dry in a drying oven at a drying temperature of 90°C-105°C and a drying time of 60-70min;

[0058] Soak the dried filter paper soaked in liquid A in liquid B, absorb the excess solution, and dry in a drying oven at a drying temperature of 70°C-90°C and a drying time of 50-60min.

[0059] The dried filter paper is cut into strips, pasted on the PVC sheet, and then cut into small strips. The size of the test paper blo...

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PUM

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Abstract

The invention relates to the technical field of medical detection and discloses a reagent for detecting urine creatinine and application of the reagent. The reagent disclosed by the invention is prepared from a liquid A and a liquid B, wherein the liquid A takes a buffer solution as a base solution and containsis prepared from phytic acid, copper sulfate, EDTA (ethylene diamine tetraacetic acid) and sodium sulfonated phthaleinsulfonamide; and the liquid B takes methanol or ethanol as a solvent and containsis prepared from TMB (tetramethylbenzidine), PVP (polyvinyl pyrrolidone) and peroxide. Byadjusting and optimizing the components in the reagent, the influence of each interference factor to detection in a urine sample is reduced, higher accuracy and precision are achieved, the linear relationship is good in a large range, the reliability is high, and the defects of poor accuracy and precision of existing likesimilar products are made up.

Description

technical field [0001] The invention relates to the technical field of medical detection, in particular to a reagent for detecting urine creatinine and an application thereof. Background technique [0002] Creatinine is a product of muscle metabolism in the human body, which is mainly excreted by glomerular filtration. 90% of the body's creatine is present in the muscles, and most of it is in the form of phosphocreatine. Creatine phosphate is dephosphorylated to produce creatinine, which is excreted in urine as creatinine. The daily excretion of urinary creatinine in normal people is quite stable. When the kidney is invaded by various causes, the kidney fibrosis causes kidney damage, and its function of excreting waste is reduced, which causes the accumulation of creatinine and other toxins in the body, resulting in a decrease in urine creatinine. Measuring urine creatinine can understand the concentration and dilution function of the kidney and the glomerular filtration ...

Claims

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Application Information

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IPC IPC(8): G01N21/78
CPCG01N21/78G01N2021/775G01N2021/7759
Inventor 崔建华沈宣辛董理韩丹丹
Owner 吉林省汇酉生物技术股份有限公司
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