Callicarpa longissima extract, preparation method and application thereof

A technology for the extraction of maple and its extract, which is applied in the field of natural product extraction, can solve the problems of few active components of maple and few active components of maple, and achieve the effects of simple preparation method, broad market prospect and easy operation

Active Publication Date: 2018-06-15
GUANGXI INST OF BOTANY THE CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] But at present, there are few researches on the maple at home and abroad, a...

Method used

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  • Callicarpa longissima extract, preparation method and application thereof
  • Callicarpa longissima extract, preparation method and application thereof
  • Callicarpa longissima extract, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Weigh 2.0mg of carnosol, 2.2mg of rosmanol and 2.5mg of isorosmanol respectively, put them together in a 10mL volumetric flask as a reference substance, add methanol to dissolve and set the volume to the mark, and then mix For the reference substance to be tested, draw 10 μL of the above-mentioned mixed reference substance to be tested, and inject it into the high performance liquid phase for determination.

[0049]Liquid chromatography conditions: Agilent 1200 liquid chromatograph, column: ZORBAX SB-C18 (5 μm, 4.6x250mm), column temperature: 30°C, flow rate: 1mL / min, detection wavelength: 210nm, mobile phase acetonitrile (A) / water (B): Gradient elution, 0-25min, 40%-74% (A).

[0050] The result is as figure 1 shown.

[0051] Depend on figure 1 It can be seen that carnosol, rosmanol and isorosmanol have good separation under the above-mentioned chromatographic conditions.

Embodiment 2

[0053] In the maple extract of this embodiment, the maple extract contains a mixture of carnosol, rosmanol and isorosmanol, wherein the sage in the maple extract The mass content of phenol is 79.6%, the mass percentage of rosmanol in the said maple japonica extract is 7.8%, and the mass percentage of isorosmanol in the maple japonica extract is 6.6% .

[0054] The preparation method of the above-mentioned maple maple extract comprises the following steps:

[0055] Step 1: Preparation of Crude Maple Extract

[0056] The stems, branches and leaves of the raw material are taken and dried, and then crushed to 2mm to obtain the dried maple powder.

[0057] Take 600g of dry maple powder, add dichloromethane 10 times the mass of maple powder, reflux extraction at 50°C for 3 times, each time for 1 hour, pass through a 30μm sieve, combine the filtrates, and concentrate the filtrate to 65°C under reduced pressure The specific gravity is the extractant of 1.02, obtains 32.2g crude ext...

Embodiment 3

[0066] In the maple extract of this embodiment, the maple extract contains a mixture of carnosol, rosmanol and isorosmanol, wherein the sage in the maple extract The mass content of phenol is 59.6%, the mass percentage content of rosmanol in the said maple japonica extract is 12.4%, and the mass percentage content of isorosmanol in the said maple maple extract is 8.0% .

[0067] The preparation method of the above-mentioned maple maple extract comprises the following steps:

[0068] Step 1: Preparation of Crude Maple Extract

[0069] The stems, branches and leaves of the raw material are taken and dried, and then crushed to 2mm to obtain the dried maple powder.

[0070] Take 600g of dried maple powder, add chloroform with 15 times the mass of maple powder, reflux and extract at 60°C for 3 times, each time for 1 hour, pass through a 50μm sieve, combine the filtrates, and concentrate the filtrate to 65°C under reduced pressure The specific gravity is the extractant of 1.05, o...

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Abstract

The invention discloses a Callicarpa longissima extract, a preparation method and the application thereof, and belongs to the technical field of natural product extraction. A mixture of carnosol, rosmanol and epirosmanol is contained in the Callicarpa longissima extract. The invention also discloses a preparation method of the Callicarpa longissima extract and the application thereof. The mixtureof carnosol, rosmanol and epirosmanol is contained in the Callicarpa longissima extract, and the components are diterpene phenol components respectively, so that the components are of various activities such as preferable oxidation resistance, cancer resistance and anti-inflammation. Through pharmacodynamics study for the Callicarpa longissima extract, a wider space is provided for further application.

Description

technical field [0001] The invention relates to a maple japonica extract, a preparation method and an application thereof, and belongs to the technical field of natural product extraction. Background technique [0002] As a free radical, nitric oxide (NO) not only functions as a second messenger and neurotransmitter, but also acts as an effector molecule to mediate the pathological and physiological processes of various diseases such as inflammation and immunity. In acute and chronic inflammation, NO is an important inflammatory factor. A large number of research results show that the levels of NO in the joint fluid and serum of patients with rheumatism are significantly higher than those of normal people. NO not only directly participates in the occurrence of rheumatism as an inflammatory mediator, but also promotes the release of other inflammatory factors, which in turn promotes the destruction of articular cartilage and aggravates the deterioration of the disease. If N...

Claims

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Application Information

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IPC IPC(8): A61K36/85A61P29/00
CPCA61K36/85A61K2236/35A61K2236/39A61K2236/51A61K2236/55
Inventor 潘争红宁德生符毓夏李连春
Owner GUANGXI INST OF BOTANY THE CHINESE ACAD OF SCI
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