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Method for determining 6-gingerol content in processing adjuvant ginger juice

A determination method, the technology of gingerol, is applied in the field of determination of 6-gingerol content in ginger juice, which is a processing auxiliary material, and can solve the problems of complex and unfavorable sample detection, and achieve high precision, simple sample processing method, and reproducible good sex effect

Inactive Publication Date: 2018-06-15
TIANSHENG PHARMA GROUP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the ingredients in ginger juice are very complex, the chromatographic peaks in the chromatogram are relatively abundant, and ginger contains a lot of cellulose, which is not conducive to the detection of samples, so there are many problems in the quality control of ginger juice in practical applications.

Method used

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  • Method for determining 6-gingerol content in processing adjuvant ginger juice
  • Method for determining 6-gingerol content in processing adjuvant ginger juice
  • Method for determining 6-gingerol content in processing adjuvant ginger juice

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Method and Results

[0023] Preparation of sample and reference solution

[0024] Preparation of the test solution

[0025] Precisely draw 0.4mL of ginger juice, put it in a 25mL measuring bottle, add absolute ethanol to dissolve and set the volume to the mark, shake well, and filter to get ready.

[0026] Preparation of reference solution

[0027] Accurately weigh 5.20mg of 6-gingerol into a 25mL measuring bottle, add absolute ethanol to dissolve and make the volume up to the mark, shake well, then precisely draw 2mL into a 10mL measuring bottle, add absolute ethanol to make up to the mark, Shake well and serve.

[0028] Selection of measurement wavelength: Scan the 6-gingerol reference solution and ginger juice test solution at 200-400nm wavelength respectively, and the result is that both have maximum absorption at 281nm.

Embodiment 2

[0029] Embodiment 2 linear relationship investigation

[0030] Accurately weigh an appropriate amount of 6-gingerol reference substance, add methanol to prepare 1.226mg·mL -l Precisely draw 1, 5, 10, 15, 18, and 20 μL of the solution, respectively, and inject samples sequentially for detection. The detection conditions are chromatographic column: Phenomenex Gemini C18 (4.6mm×250mm, 5um); flow rate: 1.0mL / min; column Temperature: 25°C; mobile phase selection: acetonitrile: methanol: water (43:5:52); measurement wavelength: 280nm. Injection volume of reference substance (mg·mL -l ) is the abscissa, and the integral value of the peak area is the ordinate, draw a standard curve and regression processing, the results show that 6-gingerol is 1.226~24.52mg·mL -l The range has a good linear relationship, and the regression equation is y=578.88x+91.297, r ＝ = 0.9999. See figure 1 Standard Curve Chart. The content determination of 6-gingerol in the ginger juice of embodiment 3

Embodiment 3

[0030] Accurately weigh an appropriate amount of 6-gingerol reference substance, add methanol to prepare 1.226mg·mL -l Precisely draw 1, 5, 10, 15, 18, and 20 μL of the solution, respectively, and inject samples sequentially for detection. The detection conditions are chromatographic column: Phenomenex Gemini C18 (4.6mm×250mm, 5um); flow rate: 1.0mL / min; column Temperature: 25°C; mobile phase selection: acetonitrile: methanol: water (43:5:52); measurement wavelength: 280nm. Injection volume of reference substance (mg·mL -l ) is the abscissa, and the integral value of the peak area is the ordinate, draw a standard curve and regression processing, the results show that 6-gingerol is 1.226~24.52mg·mL -l The range has a good linear relationship, and the regression equation is y=578.88x+91.297, r ＝ = 0.9999. See figure 1 Standard Curve Chart. The content determination of 6-gingerol in the ginger juice of embodiment 3

[0031] Taking 6-gingerol as an index component, the conte...

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Abstract

The invention provides a method for determining 6-gingerol content in a processing adjuvant ginger juice. According to the invention, methanol is taken as an extract, a sample is prepared through two-time ultrasonic treatment, and is detected through high performance liquid chromatography, and the 6-gingerol content in the ginger juice can be calculated according to a regression equation being y=578.88x+91.297 and r=0.9999. The sample processing method is simple, the 6-gingerol content in a sample solution is stabilized in at least 24 hours, method reappearance is good, precision is high, andthe method is suitable for large-area popularization.

Description

technical field [0001] The invention belongs to the technical field of detection of traditional Chinese medicine components, and in particular relates to a method for determining the content of 6-gingerol in ginger juice, a processed auxiliary material. Background technique [0002] Ginger is the fresh rhizome of ginger (Zingiber officinale Roscoe), a perennial herb of the Zingiberaceae family. The rhizome is irregular and massive, with a yellowish-brown or gray-brown surface. Traditional Chinese medicine believes that the skin of ginger is pungent and cool, which can cure skin edema and promote skin water; ginger juice is pungent and warm, and has a strong power to dispel stomach cold, and is mostly used for vomiting; Big, Pao Jiang tastes hard and goes inside but not outside, warming the coldness of the scorcher; Ginger is pungent and dissipates temperature, benefits the spleen and stomach, is good at warming the middle and reducing adverse reactions to stop vomiting, deh...

Claims

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Application Information

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IPC IPC(8): G01N30/06G01N30/02
CPCG01N30/02G01N30/06
Inventor 刘群黄勤挽谈宗华吴统选覃艺幸慧
Owner TIANSHENG PHARMA GROUP
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