Deep hypothermic circulatory rat animal model

An animal model and deep low temperature technology, applied in the field of rat models, can solve problems such as limited application, complicated operation, and long cycle, and achieve satisfactory results, simple operation, and short cycle

Inactive Publication Date: 2018-06-19
朱耀斌
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the dual effects of this technology on frostbite injury and brain protection limit the further clinical application of this technology.
At present, the animal models of domestic and foreign experimental research mostly use larger animals such as pigs, dogs, sheep, and monkeys as the research objects, and there are problems such as complicated operations, high costs, long cycles, and multidisciplinary collaboration.
[0003] To sum up, the prob...

Method used

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  • Deep hypothermic circulatory rat animal model

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Embodiment Construction

[0030] In order to further understand the content, features and effects of the present invention, the following examples are given, and detailed descriptions are given below with reference to the accompanying drawings.

[0031] The structure of the present invention will be described in detail below in conjunction with the accompanying drawings.

[0032] The animal model of deep hypothermic circulatory arrest rats provided in the embodiments of the present invention is as follows: prepare 60 healthy male rats with a body weight of 350-450 g, and divide them into 10 groups at random; 2 control groups and 8 experimental groups;

[0033] Give the prepared aqueous solutions of 2%, 5%, 10%, and 15% brain protection solution to different groups in the experimental group to drink for 7 to 15 days; record them as: T1, T2, T3, and T4 respectively;

[0034] During this period, the respiratory rate, airway pressure, arterial pressure and heart rate changes of the rats were regularly meas...

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Abstract

The invention belongs to the technical field of rat models, and discloses a deep hypothermic circulatory rat animal model. According to the model, 60 healthy male rats with the weight of 350-450 g areprepared, the respiratory frequency, airway pressure, arterial pressure and heart rate change of the rats are measured, blood enters a venous kettle through a venous drainage pipe, and the blood is pumped into a micro oxygenator through a digital flow-controllable transfer pump; after a priming solution and a brain protection solution are prepared, circulation is stopped for 60 minutes in a precise constant-temperature bath tank and in an in-vitro ice crumble environment, brain protection tissue is discontinuously subjected to antegrade cerebral perfusion protection, then circulation is restored, the body surface and the blood are subjected to rewarming at the same time, and finally a measurement result is obtained. The model is easy to operate, consumes less manpower and material resources, is short in period, simple, reliable, economical and practical, and has great research significance.

Description

technical field [0001] The invention belongs to the technical field of rat models, in particular to a rat animal model of deep hypothermic circulatory arrest. Background technique [0002] In 1951, extracorporeal circulation technology was first applied in cardiac surgery. With the development of extracorporeal circulation technology, its clinical application has become more and more extensive. Not only in thoracic surgery, but also in neurosurgery, it is applied to the treatment of giant intracranial aneurysms and vascular malformations. However, the dual effects of this technology on frostbite injury and brain protection limit the further clinical application of this technology. At present, the animal models of domestic and foreign experimental research mostly use larger animals such as pigs, dogs, sheep, and monkeys as the research objects, and there are problems such as complicated operations, high costs, long cycles, and multidisciplinary collaboration. [0003] To su...

Claims

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Application Information

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IPC IPC(8): A61D7/00A61K36/9068A61P17/02A61P25/00A61K31/125A61K35/618
CPCA61D7/00A61K31/125A61K35/618A61K36/17A61K36/185A61K36/258A61K36/284A61K36/31A61K36/344A61K36/39A61K36/43A61K36/46A61K36/481A61K36/484A61K36/537A61K36/54A61K36/714A61K36/739A61K36/752A61K36/804A61K36/9068A61K2300/00
Inventor 朱耀斌李志强刘扬樊星丁楠伊寒露
Owner 朱耀斌
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