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Method and culture medium for efficient in vitro rapid propagation of clematis

A technology of clematis and culture medium, which is applied in horticultural methods, botanical equipment and methods, horticulture, etc. It can solve the problems of long germination time, less fruiting of clematis, low seed germination rate, etc., and achieve phenotypic consistency high effect

Active Publication Date: 2018-06-22
SHANGHAI BOTANICAL GARDEN
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, clematis has less fruit, long germination time, and low seed germination rate, while the tissue culture regeneration system has the advantages of high reproduction coefficient, cycle end, high genetic stability, low cost and large-scale application. It is particularly important to cultivate industrialized seedling raising system

Method used

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  • Method and culture medium for efficient in vitro rapid propagation of clematis
  • Method and culture medium for efficient in vitro rapid propagation of clematis
  • Method and culture medium for efficient in vitro rapid propagation of clematis

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Embodiment Construction

[0030] The specific steps of the in vitro rapid propagation method of clematis are as follows:

[0031] (1) Preparation of clematis culture medium (TXL): Prepare a basic culture medium according to Table 1, pH 5.8, and sterilize at 121°C for 15-25 minutes. Adventitious shoots are induced and elongated using this medium.

[0032] Table 1 List of components of clematis culture medium (TXL)

[0033]

[0034] (2) Material: newly extracted branches or flower buds of clematis.

[0035] (3) Disinfection: Wash the fresh branches with tap water, absorb the surface water droplets, disinfect with 70% ethanol for 1-2 minutes on an ultra-clean workbench, and soak in 0.1% mercury for 8-20 minutes, sterile Rinse with water for 5-10 times, absorb the water droplets on the surface of the sterile material with sterile filter paper, remove the stems, keep the stem nodes, and cut into stem segments about 1 cm in length.

[0036] (4) Inoculate the cut sections of the stem section obtained in step (3) in T...

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Abstract

The invention discloses a method and a culture medium for efficient in vitro rapid propagation of clematis. According to the present invention, with the method and the culture medium, a large number of high-quality clematis seedlings can be rapidly propagated and produced, the occurrence of the seedlings is not limited by seasons, the seedlings can be produced all year round, the phenotypic consistency of regenerated plants is high, and the transgenic plant can be further prepared by using the regenerated system as the transgenic receptor system, such that the obtained plant can retain the genetic phenotype of the parent plant, and further can have the trait of the transfected exogenous target gene.

Description

Technical field [0001] The invention relates to a method for propagation of clematis, in particular to a method for rapid propagation of clematis in vitro and its culture medium. Background technique [0002] Clematis is the general name of Clematis L. plants in Ranunculaceae, and is a perennial woody or herbaceous vine. Clematis is a good ornamental plant, with rich varieties, gorgeous flowers, long flowering period, strong vines, strong adaptability, and good climbing characteristics. It enjoys the reputation of "Queen of Fujimoto". Clematis is a traditional medicinal plant. The root or whole herb is used as medicine. It is rich in flavonoids, coumarin and phenolic acid compounds and other effective ingredients. It is the backbone of traditional Chinese herbal medicine and ethnic Chinese medicine (Mongolian medicine, She medicine, etc.) with anti-cancer and anti-cancer effects. Clematis is mostly a good xerophyte plant. The clematis in my country's Mongolia, Gansu, Ningxia an...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 奉树成朱木兰莫健彬高燕刘航
Owner SHANGHAI BOTANICAL GARDEN
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