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A kind of high-efficiency in vitro rapid propagation method and culture medium of clematis

A technology of clematis and medium, which is applied in the field of high-efficiency in vitro rapid propagation of clematis and its medium, can solve the problems of long germination time, high genetic stability, low seed germination rate, etc., and achieve phenotype Consistent effect

Active Publication Date: 2021-09-03
SHANGHAI BOTANICAL GARDEN
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, clematis has less fruit, long germination time, and low seed germination rate, while the tissue culture regeneration system has the advantages of high reproduction coefficient, short cycle, high genetic stability, low cost and large-scale application. It is particularly important to cultivate industrial seedling raising system

Method used

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  • A kind of high-efficiency in vitro rapid propagation method and culture medium of clematis
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  • A kind of high-efficiency in vitro rapid propagation method and culture medium of clematis

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Experimental program
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Embodiment Construction

[0030] The specific operation steps of Clematis in vitro rapid propagation method are as follows:

[0031] (1) Preparation of clematis culture medium (TXL): prepare basic medium according to Table 1, pH 5.8, and sterilize at 121° C. for 15-25 minutes. Both induction and elongation of adventitious buds use this medium.

[0032] The composition list of table 1 clematis culture medium (TXL)

[0033]

[0034] (2) Materials: newly drawn branches or flower buds of clematis.

[0035] (3) Disinfection: wash the fresh branches with tap water, blot the surface water droplets, disinfect with 70% ethanol on the ultra-clean workbench for 1-2 minutes, soak in 0.1% mercury chloride for 8-20 minutes, and aseptically Rinse with water for 5-10 times, dry the water droplets on the surface of the sterile material with sterile filter paper, remove the stem, keep the stem nodes, and cut into stem segments with a length of about 1 cm.

[0036] (4) After inoculating the cut section of the stem ...

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Abstract

The invention discloses a high-efficiency in vitro rapid propagation method of clematis and its culture medium. By using the method and its culture medium, a large number of high-quality seedlings of clematis can be rapidly propagated, and the occurrence of such seedlings is not affected. Restricted by season, it can be produced all year round, and the phenotype of the regenerated plants is highly consistent. This regeneration system can be used as a transgene recipient system to further prepare transgenic plants, so that they can not only maintain the genetic phenotype of the parents, but also have the transferred foreign source. traits of the target gene.

Description

technical field [0001] The invention relates to a propagation method of clematis, in particular to a high-efficiency in vitro rapid propagation method of clematis and a culture medium thereof. Background technique [0002] Clematis is a general term for plants of the genus Clematis L. in the family Ranunculaceae, which are perennial woody or herbaceous vines. Clematis is a good ornamental plant with rich varieties, bright colors, long flowering period, strong branches and tendrils, strong ability to grow, and good climbing characteristics. It enjoys the reputation of "Queen of Fujimoto". Clematis is also a traditional medicinal plant. The root or whole herb is used as medicine. It is rich in flavonoids, coumarins and phenolic acid compounds and other active ingredients. It has anti-cancer and anti-cancer effects, and is the backbone of traditional Chinese herbal medicine and ethnic Chinese medicine (Mongolian medicine, She medicine, etc.). Clematis are mostly good xerophyt...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/00A01H4/00
CPCA01H4/001A01H4/008
Inventor 奉树成朱木兰莫健彬高燕刘航
Owner SHANGHAI BOTANICAL GARDEN
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