A method for high-efficiency in vitro regeneration of larch needles

A technology of in vitro regeneration and larch, applied in plant regeneration, horticultural methods, botanical equipment and methods, etc., can solve the problems of source limitation, no regeneration system found, regeneration effect to be improved, etc., and achieve high phenotypic consistency Effect

Active Publication Date: 2021-05-14
CAS CENT FOR EXCELLENCE IN MOLECULAR PLANT SCI
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Problems solved by technology

[0004] The current research mainly focuses on zygotic embryos or hypocotyls as explants, whose source is easily limited, and the regeneration effect needs to be improved, and the practicability is not strong enough to be widely used.
It can be seen that there is still no effective regeneration system found, which has always been a difficult problem to be overcome.

Method used

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  • A method for high-efficiency in vitro regeneration of larch needles
  • A method for high-efficiency in vitro regeneration of larch needles
  • A method for high-efficiency in vitro regeneration of larch needles

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Experimental program
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Embodiment

[0040] The present embodiment carries out according to the following steps:

[0041] (1) Preparation of LYS medium: prepare basic medium according to Table 1, pH 5.8, and sterilize at 121° C. for 15-25 minutes. The medium was used for induction, elongation and rooting of adventitious buds.

[0042] The list of components of the LYS medium used in the present embodiment of table 1

[0043]

[0044]

[0045] (2) Material: young and tender long white larch branches in the greenhouse.

[0046] (3) Disinfection: Take the young Changbai larch branches and wash them with detergent, rinse them with tap water, absorb the surface moisture of the materials with newspapers, disinfect them with recycled alcohol for 40 seconds on the ultra-clean workbench, and then disinfect them with 70% alcohol 30-90 seconds, 0.1% mercury liter disinfection for 5 minutes, rinsed with sterile water 6 times, blotted the surface moisture of the material with sterile filter paper, and cut out the stem...

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Abstract

The invention discloses a method for high-efficiency in vitro regeneration of larch needles. The larch needles are used as a starting material to produce a large number of adventitious buds after cultivation, and a large number of larch regenerated plants can be obtained in a short time. The plant phenotype The consistency is high, and the occurrence of this seedling is not limited by seasons. The regeneration system can be used as a transgene recipient system to further prepare transgenic plants, so that they can not only maintain the genetic phenotype of the parents, but also have the traits of the transferred exogenous target gene. By establishing an in vitro regeneration method with larch needles as the starting explants, research on the genetic improvement of conifers represented by larch, the cultivation of new varieties (lines) and the propagation of high-quality seedlings will contribute to the sustainable development of forestry economy. It is of far-reaching significance to narrow the gap between the field of forestry basic research and developed countries.

Description

technical field [0001] The invention relates to the field of plant regeneration and rapid technology, in particular to the in vitro regeneration of larch. Background technique [0002] Larix Mill (Larix Mill) is a deciduous tree belonging to the genus Larix of the Pinaceae family. It is widely distributed, grows quickly, has strong adaptability, and has few pests and diseases. It is valued and widely used in forestry production. At the same time, larch wood has the advantages of long fiber, decay resistance, compression resistance and bending resistance, so larch can not only be used as a high-quality building material, but also as a basic raw material for high-grade printing paper. There are 18 species of larch, which are mainly distributed in temperate mountainous areas, cold temperate plains and alpine climate zones in the northern hemisphere. There are 10 species in my country, which are mainly distributed in Northeast China, Xiaoxing'an Mountains, North China, Northwes...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 朱木兰郑珂媛王可佳闫学彤
Owner CAS CENT FOR EXCELLENCE IN MOLECULAR PLANT SCI
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