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Application of gene SOX10 as target in preparation of medicines for delaying adaptive drug resistance of melanoma

A melanoma, adaptive technology, applied in the field of biomedicine, to achieve the effect of promoting the FOXD3/ERBB3/AKT signaling pathway, promoting cell apoptosis, and delaying the adaptive drug resistance of melanoma

Active Publication Date: 2018-06-26
XI AN JIAOTONG UNIV
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  • Abstract
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  • Application Information

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Problems solved by technology

However, the key scientific question of how FOXD3 is rapidly induced by RAF inhibitors remains unanswered

Method used

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  • Application of gene SOX10 as target in preparation of medicines for delaying adaptive drug resistance of melanoma
  • Application of gene SOX10 as target in preparation of medicines for delaying adaptive drug resistance of melanoma
  • Application of gene SOX10 as target in preparation of medicines for delaying adaptive drug resistance of melanoma

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Embodiment Construction

[0021] The present invention will be further described in detail below in conjunction with specific embodiments, which are explanations of the present invention rather than limitations.

[0022] The experimental means and experimental operations required by the present invention are provided below.

[0023] 1. Cell Culture

[0024] Carry BRAF V600E The mutated melanoma cell lines 1205Lu, A375 and M238 and HEK293T cells were cultured in a constant temperature incubator at 37°C (containing 5% CO 2 ), wherein A375 and HEK293T cells were cultured in DMEM medium, and 1205Lu and M238 cells were cultured in RPMI 1640 medium. The medium contained 10% fetal bovine serum, penicillin (100 units / ml) and streptomycin (100 micrograms / ml).

[0025] 2. Western blotting

[0026]Total protein was extracted from cell lysate, separated on SDS-PAGE gel, and printed onto PVDF membrane. After blocking with 1% bovine serum albumin for 1 hour, the specific primary antibody was incubated overnight...

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Abstract

The invention discloses the application of a gene SOX10 as a target in the preparation of medicines for delaying the adaptive drug resistance of melanoma. It is disclosed that the SOX10 gene is a keyregulator of how an inhibitor induces FOXD3 expression to produce adaptive drug resistance; and further experiments indicate that down-regulation of SOX10 expression can effectively inhibit the FOXD3 / ERBB3 / AKT signaling pathways and significantly promote inhibitor-induced cells apoptosis and down-regulation of SOX10 expression significantly promotes the tumor suppression effect of RAF inhibitors and significantly inhibits the FOXD3 / ERBB3 / AKT signaling pathway in tumor tissues. Therefore, the gene SOX10 can be used as the target in the preparation of the medicines for delaying the adaptive drugresistance of melanoma, to prepare better and more medicines that delay the adaptive drug resistance of melanoma.

Description

technical field [0001] The invention belongs to the technical field of biomedicine and relates to the application of gene SOX10 as a target in the preparation of drugs for delaying adaptive drug resistance of melanoma. Background technique [0002] In recent years, due to environmental pollution, ozone layer destruction and other reasons, melanoma has increasingly become one of the major diseases that seriously threaten human health and life. The research and development of its therapeutic drugs and methods has always been the focus of research in the field of life sciences. [0003] The BRAF gene is a member of the RAF family and encodes a serine / threonine protein kinase. According to studies, 40-60% of European and American melanoma patients carry BRAF kinase mutations, among which the 600th codon valine to glutamic acid mutation (BRAF V600E ) accounted for about 90%. BRAF V600E Mutations lead to abnormal activation of BRAF kinase and continuous activation of the RAF / ME...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/136C12Q2600/158
Inventor 刘健康邵永平韩戍君
Owner XI AN JIAOTONG UNIV
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