A fast sorting device and method for male and female silkworm chrysalis
A sorting device and silkworm chrysalis technology are applied in the field of devices for identifying male and female silkworm chrysalis, can solve problems such as slow identification speed, and achieve the effects of convenient detection, fast spectrum collection speed and low price
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Embodiment 1
[0061] Build the spectrum acquisition part. Using fiber optic spectrometer, wavelength range 815-1075nm, 1240 wavelength variables. C# language is used to develop spectrum acquisition software to realize spectrum acquisition. Collect the dark current, turn on the light source, and use the polytetrafluoroethylene white board as the reference spectrum.
[0062] The original silkworm chrysalis of silkworm Jingsong A variety were taken, 96 females and 100 males, and spectra were collected once a day until moths turned into moths. Belt rotation speed 5cm / s. A fiber optic spectrometer (MAYA type, Oceanoptics, USA) was used with a wavelength range of 815-1075nm and 1240 wavelength variables. Set the spectrometer integration time to 15ms, and the number of spectral scans to 6,
[0063] On the basis of OminiDriver 2.12, use C# language to develop spectrum acquisition software to realize spectrum acquisition. Collect the dark current, turn on the light source, and use the polytetra...
Embodiment 2
[0065] The original silkworm chrysalis of several silkworm varieties were collected, including one local variety (Dazao), three original varieties used in production (Jingsong A, Su, Hu) and two hybrid original species (Ye B*Jingsong A, Su*zhen), 100 of each variety, half male and half male.
[0066] Spectral wavelength range 813-1075nm, 1250 wavelength variables. C# language is used to develop spectrum acquisition software to realize spectrum acquisition.
[0067] Collect the dark current, turn on the light source, and use the polytetrafluoroethylene white board as the reference spectrum. Belt rotation speed 20cm / s. A fiber optic spectrometer (MAYA type, Oceanoptics, USA) was used with a wavelength range of 813-1075nm and 1250 wavelength variables. Set the integration time of the spectrometer to 8 ms, the number of spectral scans to 3, and collect the spectrum once a day until moths turn into moths. The resulting spectrum is 5962, such as Image 6 shown.
Embodiment 3
[0069] In Example 2, female pupae are set as 2, and male pupae are set as 1. The spectrum is preprocessed by standard normal variable transformation, and the transformed spectrum is as follows Figure 7 shown.
[0070] The model is modeled by the PLS method, cross-validated by means of random blocks, and the factor number 6 is used when the RMSECV is the smallest, and the model is saved. The cross-validation results of male and female silkworm chrysalis were respectively fitted with normal distribution, such as Figure 8 , Figure 9 shown.
[0071] According to the probability distribution curve, the value M90 of 90% probability distribution of male silkworm chrysalis is 1.445, and the value F10 of 10% probability distribution of female silkworm chrysalis is 1.620. If the calculation result is less than 1.445, it is judged as male, if it is greater than 1.620, it is judged as female, and if it is in the middle, it cannot be judged.
[0072] Load the preprocessing method...
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