Siphoviridae as well as obtaining method and application thereof

A phage and long-tail technology, applied in the fields of stomatology and virology, can solve problems such as not many studies

Inactive Publication Date: 2018-06-29
SHANGHAI NINTH PEOPLES HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

There are not many relevant studies on the status and role of phages in the occurrence and development of periodontal disease, and there are still debates

Method used

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  • Siphoviridae as well as obtaining method and application thereof
  • Siphoviridae as well as obtaining method and application thereof
  • Siphoviridae as well as obtaining method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Example 1 The full-length gene sequence of long-tailed bacteriophage obtained from the oral cavity of periodontitis patients using viral metagenomics

[0031]Take about 2g of gingival tissue, put it into a 2ml centrifuge tube, add 1ml of PBS and 3 steel balls with a diameter of 0.3cm, seal it with a parafilm, and then homogenate (repeat freezing and thawing three times and homogenize for 5 minutes). Centrifuge at 13,000g for 10 minutes at 4°C, extract the supernatant sample, and centrifuge again at 12,000g for 5 minutes at 4°C, extract the supernatant sample, and centrifuge twice to remove cell debris and other microorganisms; process the supernatant sample through a 0.45m filter, Centrifuge and filter at 7500rpm for 1min to remove the non-viral capsicles in the suspension again; bathe in water at 37°C for 90min, apply Turbo DNase, RNase, Baseline Zero DNase and Benzonase to remove the genetic material outside the viral particle envelope. So far, 66 samples from the cas...

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Abstract

The invention discloses a siphoviridae. The siphoviridae contains nucleotide sequence for enclosing an amino acid sequence shown as SEQ ID No. 1. The invention also discloses an obtaining method and application of the siphoviridae. The siphoviridae disclosed by the invention is a novel virus obtained by being separated from an oral cavity of a periodontitis patient by adopting a viral metagenomicsmethod and provides a new basis for an etiology, treatment and prevention strategy of oral diseases.

Description

technical field [0001] The invention relates to the technical fields of stomatology and virology, in particular to a long-tailed bacteriophage isolated from the oral cavity of a periodontitis patient, its obtaining method and application. Background technique [0002] Periodontal disease is a group of complex infectious diseases, which are generally believed to be caused by bacterial stimulation and involved in many factors such as human immunity. Periodontal disease, with alveolar bone resorption, tooth loosening and loss as the main clinical symptoms, has become the main cause of adult tooth loss. Severe periodontitis not only affects oral health, but is also related to systemic diseases such as diabetes, cardiovascular disease, low birth weight newborns and premature births, which has a serious negative impact on systemic health. The traditional research on the etiology of periodontal disease usually believes that the pathogenesis of periodontal disease is related to spe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/00C12Q1/70C12Q1/686C12R1/92
CPCC12N7/00C12N2795/10321C12Q1/701
Inventor 张羽单同领冯希平李飞祝策陈曦叶玮于阗
Owner SHANGHAI NINTH PEOPLES HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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