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A kind of micro-grafting method for grafting and rooting of apple tissue culture seedlings outside the bottle

A technology of tissue cultured seedlings and micro-grafting, which is applied in the biological field, can solve the problems that restrict the scale of tissue cultured grafted seedlings, industrial production, high production cost, and difficult operation, so as to save manpower and material resources, low production cost and high success rate. high effect

Active Publication Date: 2020-04-14
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the tissue-cultured seedlings are transplanted and cultured in special medium for many times under sterile conditions, the production cost is high, and the operation is difficult and the speed is relatively slow, which restricts the large-scale and industrialized production of tissue-cultured grafted seedlings

Method used

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  • A kind of micro-grafting method for grafting and rooting of apple tissue culture seedlings outside the bottle
  • A kind of micro-grafting method for grafting and rooting of apple tissue culture seedlings outside the bottle

Examples

Experimental program
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Embodiment 1

[0024] The method of post-rooting grafting of tissue cultured apple seedlings described in this embodiment includes six steps of apple tissue culture seedling succession, rooting preparation, apple seedling rooting, rooting period management, apple seedling grafting, and grafting culture period management. Proceed as follows:

[0025] 1. Subculture of apple tissue cultured seedlings: Under aseptic conditions, cut the stems of apple tissue cultured seedlings cultured for 30-35 days into 0.5-1cm, and insert the subculture medium radially. At a temperature of 24℃, the photoperiod is Subculture at 16 / 8h for 40 days;

[0026] The subculture medium is composed of 6-BA with a final concentration of 0.5 mg / L, 0.2 mg / L IAA, MS medium, coagulant, sucrose and water, and the pH of the medium is adjusted to 5.8. The coagulant is agar, and the final concentration of the coagulant in the culture medium is 7.5 g / L.

[0027] 2. Preparation for rooting: Choose a flat-bottomed pallet container with a...

Embodiment 2

[0033] The method for simultaneously grafting and rooting apple tissue cultured seedlings described in this embodiment includes four steps: apple tissue culture seedling succession, grafting and rooting preparation, apple seedling grafting and rooting, and grafting rooting period management. The specific steps are as follows:

[0034] 1. Subculture of apple tissue culture seedlings: Under aseptic conditions, cut the stems of apple tissue culture seedlings cultured for 30-35 days into 0.5-1 cm, insert the subculture medium radially, and subculture for 40 days;

[0035] 2. Preparation for grafting and rooting: Choose a flat-bottomed pallet container of convenient size and transport, the height of the flat-bottomed pallet container is 6cm, and the domesticated rooting container (4cm×4cm, 6×12, including 72 in total) and rooting substrate (same example) 1) Mix with water, fill the wet rooting substrate into the rooting container, the thickness of the substrate is the same as the height ...

Embodiment 3

[0039] The method for grafting and rooting apple tissue culture seedlings outside the bottle described in this embodiment includes the following six: apple tissue culture seedling succession, apple seedling grafting, grafting culture period management, grafted seedling rooting preparation, grafted seedling rooting, and rooting culture period management. The specific steps are as follows:

[0040] 1. Subculture of apple tissue culture seedlings: Under aseptic conditions, cut the stems of apple tissue culture seedlings cultured for 30-35 days into 0.5-1 cm, insert the subculture medium radially, and subculture for 40 days;

[0041] 2. Grafting of apple seedlings: select rooting apple seedlings (Pingyi sweet tea) as the rootstock, cut off the top leaves, keep the stem with the root up to 2cm in length, and cut 0.4-0.5cm radially from the incision; select the vigorous and consistent growth Gala is used as a scion. Keep the top 1.5cm stem and part of the leaves, cut the base into a wedg...

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Abstract

The invention provides a micro-grafting method for an apple tissue culture seedling to strike a root in vitro through grafting. The micro-grafting method comprises the steps of apple tissue culture seedling serial subcultivation, root striking preparation, apple seedling rhizogenesis, rooting period management, apple seedling grafting and grafting cultivation period management. The method simulates the grafting process of apple branches, scions and stocks can be fully combined, root striking can be performed in a less open and moderate-humidity substrate, then plastic film is withdrawn gradually, grafted seedling acclimation is carried out, and a substrate cultivation stage is directly carried out. The micro-grafting method for the apple tissue culture seedling to strike the root in vitrothrough the grafting has the advantages that grafting out of a bottle for apple tissue culture seedling is realized, the stability of the grafted seedling is high, lots of manpower and materials are saved, production processes are simplified, the production cost is reduced, the technical method is simple, the production cost is low, the success rate is high, and direct economic benefits can be reflected in production.

Description

Technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to a micro-grafting method for grafting and rooting of apple tissue culture seedlings outside the bottle. Background technique [0002] The tissue culture micro-grafting technology was founded by Murashige. It is a combination of tissue culture and grafting, and grafting different species of rootstocks and scion tissue culture seedlings under tissue culture conditions. It has been widely used in the research and production of a variety of fruit trees because of its advantages such as less test materials, land saving, and convenient research. However, the tissue cultured seedlings are repeatedly cultured in a special medium under sterile conditions, and the production cost is high, and the operation is difficult, and the speed is relatively slow, which restricts the large-scale and industrialized production of tissue cultured grafted seedlings. Summary of the invention [0003] In ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G2/30A01H4/00
CPCA01H4/001A01H4/008
Inventor 张世忠郭亚蓉郭成于艳艳张甜甜
Owner SHANDONG AGRICULTURAL UNIVERSITY