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A kind of method for preparing d-borneol

A technology of d-borneol and camphor, which is applied in the field of preparation of d-borneol, can solve the problems of low industrialization value, and achieve the effects of mild conditions, cost reduction and simple operation

Active Publication Date: 2020-10-09
SUZHOU LEAD BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For example, the Guangzhou Institute of Chemistry, Chinese Academy of Sciences reported in 1987 (Guangzhou Chemical Industry, 1987, 3, 12-15), using a high degree of optical rotation D-turpentine as a raw material to synthesize D-borneol, although borneol and isoborneol The ratio is as high as 94:6, and the specific curl is above 25, but the yield of the reaction is only about 20%, and the industrialization value is not great

Method used

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  • A kind of method for preparing d-borneol
  • A kind of method for preparing d-borneol
  • A kind of method for preparing d-borneol

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 2.5 g of dextro camphor and 3.6 g of glucose were placed in a 100 mL three-necked flask, and 50 mL of pH=6.5, 0.05M PBS buffer was added. Put the three-necked flask into the reaction pot, set the rotation speed to 850 rpm and the temperature to 30°C. Then add 5mg NADP separately + , 100mg glucose dehydrogenase (purchased from Suzhou Yinhang Biotechnology Co., Ltd.: product code YH1901), and 100mg ketoreductase powder (purchased from Suzhou Yinhang Biotechnology Co., Ltd.: product code YH2022). The reaction started. During the reaction, the pH was maintained at about 6.5 with a 2M NaOH solution and monitored by HPLC. Reaction conversion rate after 24 hours> 99%, dextroborneol: isoborneol 97:3.

Embodiment 2

[0028] 2.5 g of dextro camphor and 3.6 g of glucose were placed in a 100 mL three-necked flask, and 50 mL of pH=6.5, 0.05M PBS buffer was added. Put the three-necked flask into the reaction pot, set the rotation speed to 850 rpm and the temperature to 30°C. Then add 5mg NADP separately + , 100mg glucose dehydrogenase (purchased from Suzhou Yinhang Biotechnology Co., Ltd.: product code YH1901), and 100mg ketoreductase powder (purchased from Suzhou Yinhang Biotechnology Co., Ltd.: product code YH2067). The reaction started. During the reaction, the pH was maintained at about 6.5 with a 2M NaOH solution and monitored by HPLC. The reaction conversion rate is 98% after 24 hours, dextroborneol: isoborneol> 99.5:0.5.

Embodiment 3

[0030] 2.5 g of dextro camphor and 3.6 g of glucose were placed in a 100 mL three-necked flask, and 50 mL of pH=6.5, 0.05M PBS buffer was added. Put the three-necked flask into the reaction pot, set the rotation speed to 850 rpm and the temperature to 30°C. Then add 5mg NADP separately + , 100mg glucose dehydrogenase (purchased from Suzhou Yinhang Biotechnology Co., Ltd.: product code YH1901), and 100mg ketoreductase powder (purchased from Suzhou Yinhang Biotechnology Co., Ltd.: product code YH2069). The reaction started. During the reaction, the pH was maintained at about 6.5 with a 2M NaOH solution and monitored by HPLC. After 24 hours, the reaction conversion rate was 95%, dextroborneol:isoborneol 96:4.

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Abstract

A method for preparing d-borneol from d-camphor under the action of ketoreductase.

Description

Technical field [0001] The invention belongs to the technical fields of biopharmaceuticals and biochemical engineering, and specifically relates to a preparation method of dextroborneol. Background technique [0002] D-borneol, also called natural borneol, is an important medicinal material. D-borneol has an important regulating effect on the excitement of people's central nervous system, and is often used as a drug to be used in the formulation of various Chinese medicines to help Chinese medicines improve their therapeutic effects. Many valuable Chinese patent medicines use it as one of the main ingredients, such as Liushen pills, Jianxin pills, Niuhuang Shangqing pills and so on. D-borneol is also a spice with a long history, often used in cosmetics and colognes. [0003] At present, D-borneol is extracted from camphor camphor, camphor camphor and other plants. The production cost is high, resources are scarce, and the market gap is very large. Synthetic borneol (containing L...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/02C12N9/04
CPCC12N9/0065C12P7/02C12N9/0006C12P5/007C12Y101/01184
Inventor 谢新开黄晓飞杜好勉
Owner SUZHOU LEAD BIOTECH CO LTD