Time-resolved fluorescent immunochromatographic test strip, kit and preparation method for detecting ck-mb

Abstract

The invention discloses a time-resolved immunochromatographic test strip for detecting CK-MB, a kit and a preparation method thereof. Pad, coating film and absorbent paper, the CK-MB monoclonal detection antibody that is coated with fluorescent microsphere mark is coated on the described binding pad, and described coating film comprises parallel setting, and mutually spaced detection area and control area, and described detection area Coated with CK-MB monoclonal capture antibody that recognizes a single antigenic epitope, the control area is coated with goat anti-mouse IgG antibody; the coated membrane is a nitrocellulose membrane combined with a polymer, and the polymer is in A material with a light transmittance of less than 10% for a wavelength of less than 450nm and a light transmittance of more than 95% for a wavelength of 500nm or more. The test strip of the invention can be used for rapid quantitative detection, the test result is accurate and reliable, the sensitivity is high, the preparation method is simple, the method is suitable for large-scale production, and it has positive significance for the quantitative detection of CK-MB.

Description

technical field [0001] The invention belongs to the technical field of medical testing, in particular, the invention relates to a time-resolved fluorescent immunochromatographic test strip, a kit and a preparation method thereof for quantitatively detecting CK-MB. Background technique [0002] Creatine Kinase (Creatine Kinase, CK) usually exists in the cytoplasm and mitochondria of animal heart, muscle, and brain tissues. It is an important kinase that is directly related to intracellular energy operation, muscle contraction, and ATP regeneration. It is reversible It catalyzes the transphosphoryl reaction between creatine and ATP. Creatine kinase isoenzyme (CK-MB) is mainly distributed in the myocardium. When the myocardium is damaged, CK-MB is released into the blood, and starts to increase after 3-8 hours, and can last for 3-5 days. The combined detection of troponin, myoglobin, and creatine kinase isoenzyme can detect myocardial damage as early as possible. Creatine ki...

AI Technical Summary

Problems solved by technology

However, the sensitivity of qualitative detection is not high

[0006] 3. Transmission immunoturbidimetry——This method is simple, fast, and can be automated, and is suitable for batch detection. However, the methodology and clinical application of immunoturbidimetry still need further verification

Various test strips and instruments based on immunochromatography, chromatography and dry chemistry techniques will affect the activity of microproteins in the matrix due to differences in temperature, humidity and pH, thereby affecting the results

The methodological flaws of some POCT instruments, such as poor sensitivity and repeatability, and narrow linear range, are only used for reference in emergencies or emergencies, and need to be sent to the laboratory for re-examination if necessary.

[0009] The current immunofluorescence chromatography device uses the reflection method to detect the fluorescent signal on the porous membrane. The fluorescence detector captures the specific antibody modified by the fluorescent dye on the surface of the porous membrane, but cannot detect the fluorescent signal inside the porous membrane, which leads to the detection of Sensitivity drop