A kind of tissue culture method of Grass officinalis
A technique of tissue culture and Tongquancao, which is applied in the field of tissue culture of T. praecox to achieve a good ecological environment and a broad market application prospect
Active Publication Date: 2021-01-12
CHANGSHA UNIVERSITY
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Problems solved by technology
At present, there are no literatures and patents related to the tissue culture of T. praecox. The application of tissue culture technology can rapidly multiply the selected high-quality T. sagocai varieties in large quantities, and maintain the excellent quality of the female parent. The application provides a large number of excellent raw materials
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Embodiment 1
[0031] A tissue culture method of Grass praecox, comprising the steps of:
[0032] 1) Leaves form callus tissue: After the leaves of T. praecox praecox picked in the field are sterilized, the veins and leaf margins are cut off, cut into small pieces of about 5 mm × 5 mm, and inoculated on the callus induction medium. Routine culture to callus formation; the induction medium of the leaf callus is the MS medium comprising 1mg / L 6-benzylaminopurine and 0.3mg / L naphthaleneacetic acid; the callus formation of the leaf The time is 20d; the sucrose concentration in the MS medium is 30g / L, and the agar powder concentration is 8g / L; the culture conditions of the conventional culture are pH 6.0, light intensity 1500lx, light time 10h / d, constant temperature The temperature of the incubator was 25°C.
[0033] 2) Differentiation of callus into adventitious buds: Cut the callus formed in step 1) into small pieces of about 5mm×5mm, inoculate them on the induction medium for differentiation...
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The invention relates to the field of plant tissue culture, and discloses a tissue culture method for mazus caducifer hance. The method comprises the following steps that 1) callus tissue forming by leaves: after the picked mazus caducifer hance leaves in the open undergo disinfection treatment, leaf veins and leaf margins are cut out, the leaves are cut into small pieces of about 5 millimeters*5millimeters, and the small pieces are inoculated on a tissue inducing culture medium and are conventionally cultured to form the callus tissue; 2) adventitious bud differentiation by the callus tissue: the formed callus tissue in the step one is cut into small pieces of about 5 millimeters*5 millimeters, and the small pieces are inoculated on a inducing culture medium of differentiating buds and are conventionally cultured to form the adventitious buds on the callus tissue; 3) adventitious bud rooting: the formed adventitious buds with sizes of about 3-5 centimeters are cut out, inoculated ona root growing inducing culture medium, and conventionally cultured to root; 4) tissue cultured seedling blooming. The method can acquire a large number of excellent tissue cultured seedlings of the mazus caducifer hance within 50 days, and the market application prospects are wide.
Description
technical field [0001] The invention relates to the field of plant tissue culture, in particular to a method for tissue culture of Grass praecox. Background technique [0002] Mazus caducifer Hance is a perennial herb of the family Scrophulariaceae, with a height of 20-50cm, strong, and the whole body is covered with multicellular white villous hairs; the racemes are terminal, up to 35cm long, and the flowering period is 4 -May; capsule spherical, fruiting period June-August; seeds brown, many and small; growing in damp roadsides, under forests, grass slopes below 1300m above sea level. The plants of the genus Tongquancao are dwarf herbs, most of which have medicinal value. They have the functions of clearing away heat and detoxification, detoxification, pain relief, and invigorating the stomach. . The plant resources of this genus are relatively rich, and they are in a wild state in most areas. It is yet to select species with excellent traits and use them in practice, su...
Claims
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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 陈建荣刘芳唐映红唐伟卓
Owner CHANGSHA UNIVERSITY