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A biological treatment method for liver cancer

A drug and composition technology, applied in the field of liver cancer biotherapy, can solve the problems of little effect and inability to prevent tumors

Active Publication Date: 2020-11-06
MEDICINE & BIOENG INST OF CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although biological therapy has shown good prospects, there are still many difficulties and problems
Sometimes, although a variety of biological response modifiers are applied, several immune indicators are expected to return to normal, but they still cannot prevent the occurrence of tumors, with little effect

Method used

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  • A biological treatment method for liver cancer
  • A biological treatment method for liver cancer
  • A biological treatment method for liver cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Construction of H22 mouse liver cancer animal model

[0024] H22 ascites tumors were frozen in our laboratory. We inoculated the H22 ascites tumors frozen in liquid nitrogen into the peritoneal cavity of Kunming mice. After 2 weeks, the peritoneal cavity of Kunming mice was covered with H22 ascites. The ascites tumor of H22 was inoculated into the armpit of Kunming mice, and after 2 weeks, it could grow into a solid transplanted tumor of H22 liver cancer.

[0025] Take the H22 ascites tumor fluid, count the cells in the ascites, and dilute to 7.5×10 6 1 / ml and inoculated 0.2ml dilution in the armpit of 10 female 20-22g Kunming mice, and another 10 healthy growing mice were used as controls. Ten days later, the two groups of mice were removed from the eyeballs to collect blood, and dissected to record the tumor tissue, liver, brown adipose tissue (Brown Adipose Tissue, hereinafter referred to as BAT) in the scapula and white adipose tissue (White Adipose Tissu...

Embodiment 2

[0035] Excision model of embodiment 2 brown adipose tissue

[0036] 40 female Kunming mice weighing 20-22 g were anesthetized by injecting pentobarbital sodium solution at 50 mg / kg, and 20 of them had the brown adipose tissue in the scapula removed, and the wound was sutured immediately; the other 20 were sham Surgical treatment, that is, suturing directly after the incision of the scapula. Three days later, 10 mice with brown adipose tissue excised and 10 sham-operated mice were inoculated with H22 tumor fluid in the armpit. Ten days later, the eyeballs of all 40 mice were removed to collect blood, and dissected to record the weights of tumor, liver, brown adipose tissue in the scapula and white adipose tissue in the groin. The results are shown in Table 3. At the end of the experiment, the collected blood was centrifuged, and the supernatant was drawn into a clean EP tube. This was the serum, which was stored in a -80°C refrigerator.

[0037] We found that the weight of tu...

Embodiment 3

[0052] Example 3 H22 mixed primary brown adipocyte (BrownAdipose Cell, hereinafter referred to as BAC) xenograft tumor model

[0053] First, cells from mouse brown adipose tissue were extracted. Take 3-4 week old Kunming mice, kill them by pulling their necks, and soak them in alcohol for 5 minutes. Take out the brown adipose tissue in the scapula, trim it as clean as possible in the ultra-clean workbench, remove the surrounding white adipose tissue, cut it into pieces and place it in the digestive solution (1mg / ml collagenase Ⅰ, 123mM NaCl, 5mM KCl, 1.3mM CaCl 2 , 5mM glucose, 4% BSA, and 100 mM Hepes, pH 7.4) for half an hour, take the strainer to filter, leave the undigested tissue on the top to continue digestion, and centrifuge the digestion solution below to reweight the cells in the pellet suspended in DMEM-F12 medium containing 20% ​​FBS. After overnight culture, brown fat cells were counted, mixed with H22 cell suspension, and each mouse was injected with 0.2ml of t...

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Abstract

The invention provides a method for treatment of liver cancer in a subject, wherein the method includes that brown adipose cells or compositions thereof are applied to the subject, a drug delivery route of the brown adipose cells is carried out through intratumoral, intraarterial, intravenous, intrapleural, intracystic, intracavitary or abdominal injection or oral administration, and the drug delivery route of the brown adipose cells comprises the step of injecting a brown adipose cell suspension to the subject. The invention also provides a method for constructing a mice liver cancer model, wherein the method comprises the step of reducing or excising brown adipose tissues in mice bodies or reducing brown adipose cells in the mice bodies; preferably, the method comprises the step of carrying out surgical excision or partial excision of the brown adipose tissues in the mice bodies; the invention also provides a method for inhibition of proliferation or metastasis of liver cancer cellsin vivo or in vitro. Inhibition of the liver cancer cells or reduction of metastasis of the liver cancer cells are achieved by contact with the liver cancer cells in vivo or in vitro or mixing with the brown adipose cells.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a novel biological treatment method for liver cancer. Background technique [0002] Liver cancer is a common malignant tumor. The current treatment methods for liver cancer include chemotherapy, radiotherapy, surgery and biological therapy. The liver is an important metabolic organ of the human body. When the liver cells become cancerous, the metabolic function of the human body is out of balance, and this unbalanced feedback will accelerate the growth of liver cancer. The existing surgical treatment has great limitations, while chemotherapy and radiotherapy have strong toxic and side effects, which reduces the quality of life of patients. Although biological therapy has shown good prospects, there are still many difficulties and problems. Sometimes, although a variety of biological response modifiers are used, several immune indicators are expected to return to normal, b...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K35/35A61P35/00C12N5/09C12N5/077
CPCA61K35/35C12N5/0653C12N5/0693C12N2502/1305C12N2502/30
Inventor 陈淑珍刘栋李毅商悦王文蝶
Owner MEDICINE & BIOENG INST OF CHINESE ACAD OF MEDICAL SCI
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