Factory culture method of phellinus igniarius fruiting bodies and culture medium thereof

The technology of culture medium and Phellinus linteus, applied in cultivation, plant cultivation, mushroom cultivation and other directions, can solve the problems of high price of finished products, high pollution rate, long cultivation period, etc., and achieve stable product quality, high active ingredients, and cultivation period. short effect

Inactive Publication Date: 2018-07-10
LUDONG UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the artificial cultivation of Phellinus japonica has been realized, and it is cultivated in South Korea, China, and Japan. The method is to use basswood or mushroom bag cultivation. It takes more than three years to harvest the fruiting bodies. Due to the long cultivation period and environmental exposure, the pollution rate is high. Uneven product quality and high finished product prices
The comprehensive factors of output, quality and price limit the further development and application of Sanghuang
[0005] In particular, the cultivation cycle of the existing Phellinus radix fruiting body is too long, and industrialized and mechanized cultivation cannot be realized, and the industrialized cultivation of Phellinus radix is ​​still a blank

Method used

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  • Factory culture method of phellinus igniarius fruiting bodies and culture medium thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: Rice is the main raw material.

[0024] (1) Strains: Phellinus strains, purchased from Guangdong Microbial Strain Collection Center, and stored on PDA slant medium.

[0025] (2) Seed preparation: under conditions suitable for Phellinus growth, cultivate Phellinus mycelia in liquid seed medium to logarithmic growth phase to obtain liquid Phellinus seeds; or cultivate Phellinus mycelium in solid seed medium , to obtain solid Phellinus phellidis seeds, its specific steps are as follows:

[0026] Activation of preserved strains: the solid seed medium used for the activation of strains is carrot agar solid medium, and after inoculation of slant strains, the temperature is 28°C, and dark culture is carried out for 8 days.

[0027] Expansion culture of solid strains: the composition of solid strain expansion medium is 20g / L corn flour, 20g / L maltose, 1g / L peptone, 0.6g / L MgSO4, 1g / L KH2P04, 15g / L agar, and Measure the water, the pH is natural; after inoculating...

Embodiment 2

[0034] Embodiment 2: with millet main raw material

[0035] (1) Strains: Phellinus strains, purchased from Guangdong Microbial Strain Collection Center, and stored on PDA slant medium.

[0036] (2) Seed preparation: under conditions suitable for Phellinus growth, cultivate Phellinus mycelia in liquid seed medium to logarithmic growth phase to obtain liquid Phellinus seeds; or cultivate Phellinus mycelium in solid seed medium , to obtain solid Phellinus phellidis seeds, its specific steps are as follows:

[0037] Activation of preserved strains: the solid seed medium used for the activation of strains is carrot agar solid medium, and after inoculation of slant strains, the temperature is 28°C, and dark culture is carried out for 12 days.

[0038] Expansion culture of solid strains: the composition of solid strain expansion medium is 15g / L corn flour, 15g / L maltose, 0.8g / L peptone, 0.5g / L MgSO4, 3g / L KH2P04, 20g / L agar, The remaining water has a natural pH; after the activated...

Embodiment 3

[0045] Embodiment 3: with oat main raw material

[0046] (1) Strains: Phellinus strains, purchased from Guangdong Microbial Strain Collection Center, and stored on PDA slant medium.

[0047] (2) Seed preparation: under conditions suitable for Phellinus growth, cultivate Phellinus mycelia in liquid seed medium to logarithmic growth phase to obtain liquid Phellinus seeds; or cultivate Phellinus mycelium in solid seed medium , to obtain solid Phellinus phellidis seeds, its specific steps are as follows:

[0048] Activation of preserved strains: the solid seed medium used for the activation of strains is carrot agar solid medium, and after inoculation of slant strains, the temperature is 28°C, and dark culture is carried out for 10 days.

[0049] Expansion culture of solid strains: the composition of solid strain expansion medium is 10g / L corn flour, 10g / L maltose, 0.5g / L peptone, 0.1g / L MgSO4, 4g / L KH2P04, 25g / L agar, The remaining water has a natural pH; after the activated Ph...

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Abstract

The invention discloses a factory culture method of phellinus igniarius fruiting bodies and a culture medium thereof. The method comprises the steps of phellinus igniarius seed preparation, factory production culture medium preparation, inoculation, culture management, harvesting and drying, wherein a solid strain amplification culture medium is prepared from corn powder, maltose, peptone, MgSO4,KH2PO4 and agar; a liquid strain amplification culture medium is prepared from glucose, yeast powder, MgSO4, KH2PO4, ammonium citrate tribasic, ramulus mori water extracts and vitamin B1; a factory production culture medium is prepared from grain seeds, distilled water, MgSO4, KH2PO4 and ramulus mori water extracts. The method and the culture medium have the advantages that the pollution rate is low; the culture period is short; the product quality is stable; the cost is low; the factory standardized production can be realized. By using the culture medium, the phellinus igniarius hyphae growsfast and exuberantly; the obtained phellinus igniarius seed quantity is large; the time is short; the raw materials are simple and can be easily bought; the price is low; the culture time of the phellinus igniarius fruiting bodies is shortened; the biological conversion efficiency is high; the contents of ingredients of polysaccharide, flavone and triterpenoids can be kept higher.

Description

Technical field: [0001] The invention relates to a method for industrialized cultivation of the fruiting body of Phellinus phellinus and its culture medium, which belongs to the technical field of cultivation of edible and medicinal fungi, and relates to a method for cultivating the famous and precious medicinal fungus Phellinus phellidis. By adopting the method, the industrialization of Phellinus phellidis can be realized , Standardization, aseptic cultivation. Background technique: [0002] Phellinus ( Phellinus igniarius ), also known as Phellinus syringae, Apinola, Phellinus, Prunus, belongs to Basidiomycotina ( Basidiomycotina ), Laminaria ( Hymenomycetes ), Polyporaceae ( Polyporaceae ), Xylopores ( Puccinia pusilla ), is a rare medicinal fungus born on the trunks of broad-leaved trees such as poplar and willow. It grows in North China, Northwest my country, Heilongjiang, Jilin, Taiwan, Guangdong, Sichuan, Yunnan, Tibet and other regions. It is widely distribut...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G1/00A01G18/00A01G18/20
CPCC05B7/00C05G5/20C05D5/00C05F11/00C05C3/00C05F11/08C05F11/10
Inventor 程显好刘凯盖宇鹏刘静李维焕
Owner LUDONG UNIVERSITY
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