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Application of 3beta-hydroxynorerythrosuamine-3-O-beta-D-glucopyranoside to preparing antitumor pharmaceutic preparation

A technology of glucopyranoside and anti-tumor drugs, which is applied in the field of natural medicines and chemical medicines to achieve good medicinal prospects, reduce the number of cell clones, and inhibit proliferation

Active Publication Date: 2018-07-20
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

3β-hydroxynorerythrosuamine-3-O-β-D-glucopyranoside (3β-hydroxynorerythrosuamine 3-O-β-D-glucopyranoside) isolated from the seeds of Glycyrrhiza glabrata selectively inhibits human tumors Cell growth, induction of tumor cell apoptosis, inhibition of cell clone formation and other anti-tumor effects and drug applications have not been reported

Method used

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  • Application of 3beta-hydroxynorerythrosuamine-3-O-beta-D-glucopyranoside to preparing antitumor pharmaceutic preparation
  • Application of 3beta-hydroxynorerythrosuamine-3-O-beta-D-glucopyranoside to preparing antitumor pharmaceutic preparation
  • Application of 3beta-hydroxynorerythrosuamine-3-O-beta-D-glucopyranoside to preparing antitumor pharmaceutic preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Inhibition of tumor cell proliferation by 3β-hydroxydesmethylglucothreamide 3-O-β-D-glucopyranoside.

[0024] Test method: cells grown in logarithmic phase (human breast cancer cell MCF-7, human breast cancer cell MDA-MB-231, human breast cancer cell MDA-MB-453, human non-small cell carcinoma cell A549, human non-small cell carcinoma cell Cell carcinoma cells NCI-H1299, human liver cancer cells HepG2, human liver cancer cells Bel-7402, human ovarian cancer cells A2780, human cervical cancer cells Hela, human colon cancer cells HCT-8, human gastric cancer cells BGC-823, human glial cells Tumor cells (U87) were digested, centrifuged, counted, and 1*10 6 cells / ml inoculated in 96-well culture plate, 100 μL per well, 37°C, 5% CO 2 After incubating in the incubator for 24 hours, add 3β-hydroxynormothreamide 3-O-β-D-glucopyranoside with a concentration gradient, and set solvent control and blank control at the same time. After 24 hours of incubation, add 20 μL to each well ...

Embodiment 2

[0033] Morphological observation of human breast cancer MCF-7 cells acted on by 3β-hydroxydesmethylglucothreamide 3-O-β-D-glucopyranoside.

[0034] Test method: cells MCF-7 (human breast cancer cells) in the logarithmic growth phase were digested, centrifuged, counted, and 1*10 5 cells / ml inoculated in 6-well culture plate, 3ml per well, 37°C, 5% CO 2 After incubating in the incubator for 18 hours, 3β-hydroxynorgamthrein 3-O-β-D-glucopyranoside (0, 2, 4 and 6 μM) was added in a gradient concentration, incubated for 48 hours, and observed under a microscope .

[0035] Test results: if figure 1 As shown, the normal cells are plump and have a strong refractive index. After adding 2 μM of 3β-hydroxydesmegamthreamide 3-O-β-D-glucopyranoside, the cells become elongated, scattered, and a few parts are round. With the increase of drug concentration, the number of rounded cells increased and the refractive index decreased. At 6 μM, some cells had already floated in the culture mediu...

Embodiment 3

[0037] Hoechst staining of human breast cancer MCF-7 cells treated with 3β-hydroxynorgamthreamide 3-O-β-D-glucopyranoside.

[0038] Test method: cells MCF-7 (human breast cancer cells) in the logarithmic growth phase were digested, centrifuged, counted, and 1*10 5 cells / ml inoculated in 6-well culture plate, 3ml per well, 37°C, 5% CO 2 After incubating in the incubator for 18 hours, add 3β-hydroxynorgamthreamide 3-O-β-D-glucopyranoside with a concentration of 4 μM. After culturing for 24 hours, suck out the culture medium, wash once with PBS, and add Hoechst dye was incubated in an incubator. After 15 minutes, the dye was sucked off, washed again with PBS, and observed with a fluorescent inverted microscope.

[0039] Test results: Under a fluorescent inverted microscope, compared with normal cells that only showed weak fluorescence, the volume of MCF-7 cells after 3-O-β-D-glucopyranoside was treated for 24 hours, smaller, chromatin shrinkage, bright blue nuclei and obvious ...

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Abstract

The invention discloses application of 3beta-hydroxynorerythrosuamine-3-O-beta-D-glucopyranoside to preparing an antitumor pharmaceutic preparation. The antitumor pharmaceutic preparation comprises apharmaceutic adjuvant and 3beta-hydroxynorerythrosuamine-3-O-beta-D-glucopyranoside at the effective dose for treatment. The 3beta-hydroxynorerythrosuamine-3-O-beta-D-glucopyranoside inhibits the growth of multiple tumor cell strains, inhibits the clone formation of cells, and induces the apoptosis of tumor cells.

Description

technical field [0001] The invention relates to the fields of natural medicine and chemical medicine, and more specifically relates to the application of 3β-hydroxynorgamuxinamide 3-O-β-D-glucopyranoside in the preparation of antitumor drug preparations. Background technique [0002] With the development of the economy, the pollution of the environment has increased, the pressure of life has increased, and the incidence of malignant tumors has also increased year by year. Cancer has also become the number one killer that endangers human life. Research and development of anticancer drugs has always been the focus of the medical community all over the world. hotspot. In the research of anticancer drugs, drugs that induce tumor cell apoptosis, inhibit tumor angiogenesis, reverse multidrug resistance of tumor cells, and inhibit tumor cell invasion and migration are the main directions of anticancer drug research and development in recent years. [0003] The seeds of Gemu are th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/704A61P35/00
CPCA61K31/704
Inventor 田海妍王勇黄秀勇许川山陈泽平
Owner JINAN UNIVERSITY
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