Disease-resisting and growth-promoting compound microbial agent and manufacturing method thereof

A composite microbial inoculum and a technology for a manufacturing method, which are applied in the field of disease-resistant and growth-promoting compound microbial inoculants and their manufacturing fields, can solve problems such as unsatisfactory and unreasonable composition of ingredients, and achieve a simple and easy manufacturing method and good disease resistance. The effect of promoting growth and improving the disease resistance of crops

Active Publication Date: 2018-07-24
STANLEY FERTILIZER SUIPING CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the compound microbial agents currently used by people have the following deficiencies: the combination of ingredients is not reasonable enough, and the dual functions of preventing and controlling fungal diseases and promoting growth that cannot meet the requirements of crops

Method used

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  • Disease-resisting and growth-promoting compound microbial agent and manufacturing method thereof
  • Disease-resisting and growth-promoting compound microbial agent and manufacturing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A disease-resistant and growth-promoting compound microbial bacterial agent, which is prepared from the following raw materials in parts by weight: 25 parts of Bacillus thuringiensis, 18 parts of Bacillus licheniformis, 13 parts of Bacillus cereus, 20 parts of Hansenula anomaly agent, defective 11 parts of Brevundimonas agent, 8 parts of Azotobacter rotundum agent, and 6 parts of cross-linked chitosan.

[0034] Described, cross-linked chitosan is made by following method:

[0035] 1) adding chitosan to an aqueous acetic acid solution with a volume fraction of 1.4%, stirring and dissolving, and adjusting the pH to 5 to obtain a chitosan raw material solution;

[0036] 2) Add Tween 80 to the chitosan raw material solution, stir for 25 minutes, add ferric sulfate while stirring, continue stirring for 10 minutes, and let stand for 30 minutes after stirring to obtain an emulsion;

[0037] 3) Add sodium tripolyphosphate aqueous solution with a volume fraction of 1.8% to the ...

Embodiment 2

[0050] A disease-resistant and growth-promoting compound microbial bacterial agent, which is prepared from the following raw materials in parts by weight: 30 parts of Bacillus thuringiensis, 15 parts of Bacillus licheniformis, 10 parts of Bacillus cereus, 15 parts of Hansenula anomaly, defective 12 parts of Brevundimonas agent, 10 parts of Azotobacter rotunda agent, and 8 parts of cross-linked chitosan.

[0051] Described, cross-linked chitosan is made by following method:

[0052] 1) adding chitosan to an aqueous acetic acid solution with a volume fraction of 1%, stirring and dissolving, and adjusting the pH to 5 to obtain a chitosan raw material solution;

[0053] 2) Add Tween 80 to the chitosan raw material solution, stir for 20 minutes, add ferric sulfate while stirring, continue stirring for 10 minutes, and let stand for 40 minutes after stirring to obtain an emulsion;

[0054] 3) Add sodium tripolyphosphate aqueous solution with a volume fraction of 1.5% to the emulsion...

Embodiment 3

[0060] A disease-resistant and growth-promoting compound microbial bacterial agent, which is prepared from the following raw materials in parts by weight: 20 parts of Bacillus thuringiensis, 20 parts of Bacillus licheniformis, 16 parts of Bacillus cereus, 25 parts of Hansenula anomaly agent, defective 10 parts of Brevundimonas agent, 6 parts of Azotobacter rotundum agent, 4 parts of cross-linked chitosan.

[0061] Described, cross-linked chitosan is made by following method:

[0062] 1) adding chitosan to an aqueous acetic acid solution with a volume fraction of 1.8%, stirring and dissolving, and adjusting the pH to 5 to obtain a chitosan raw material solution;

[0063] 2) Add Tween 80 to the chitosan raw material solution, stir for 30 minutes, add ferric sulfate while stirring, continue stirring for 10 minutes, and let stand for 20 minutes after stirring to obtain an emulsion;

[0064] 3) adding 2% sodium tripolyphosphate aqueous solution into the emulsion, stirring for 5 mi...

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Abstract

The invention discloses a disease-resisting and growth-promoting compound microbial agent, which is prepared from the following raw materials in parts by weight: 20-30 parts of bacillus thuringiensis,15-20 parts of bacillus licheniformis, 10-16 parts of bacillus cereus, 15-25 parts of hansenula anomala, 10-12 parts of brevendimonas diminuta, 6-10 parts of azotobacter chroococcum and 4-8 parts ofcross-linked chitosan. The invention also discloses a manufacturing method of the disease-resisting and growth-promoting compound microbial agent. The disease-resisting and growth-promoting compound microbial agent relies on the mutual coordination and proliferation relationship among all the strains so as to form a biological flora which is complex, stable in structure and wide in functions and has a variety of bacterial microbial communities; the formed biological flora is rapidly combined with positive power in the soil so as to produce antioxidant substances, remove oxidizing substances and inhibit pathogenic bacteria; therefore, a good environment suitable for crop growth is formed, and the disease resistance of crops is improved.

Description

technical field [0001] The invention relates to the field of microbial bacterial agents, in particular to a disease-resistant and growth-promoting compound microbial bacterial agent and a manufacturing method thereof. Background technique [0002] With the whole society's emphasis on food safety and the development of sustainable agriculture, the use of safe, efficient and non-environmentally polluting microbial agents to prevent and control plant diseases has been generally accepted by people. The mechanism of action of bio-control microorganisms mainly includes the following aspects: some microorganisms can produce some antibiotics that can antagonize pathogenic bacteria; some microorganisms can produce bactericidal proteins to kill pathogenic bacteria; some microorganisms can produce chitinase, which can Plays a biocontrol role by lysing the spore wall of fungal pathogens; some microorganisms can induce disease resistance in plants. Due to the advantages of broad antibac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16A01N63/04A01N63/00A01N43/16A01P21/00A01P1/00A01P3/00C12R1/07C12R1/10C12R1/085C12R1/78C12R1/01C12R1/065
CPCA01N43/16A01N63/00A01N63/30C12N1/16C12N1/20
Inventor 彭永博王秀平吴冰冰
Owner STANLEY FERTILIZER SUIPING CO LTD
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