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Method for goose paternity test

A goose paternity and microsatellite marker technology, applied in the field of goose paternity identification, can solve problems such as unclear pedigree and difficulty in individual identification

Active Publication Date: 2018-07-24
HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a kind of method that is applied to goose paternity identification in view of the above-mentioned problem, is used for solving goose breeding process, due to large group mating, different parental eggs mix Difficulties in individual identification and unclear pedigree

Method used

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  • Method for goose paternity test

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Experimental program
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Embodiment Construction

[0006] In order to illustrate the technical solution of the present invention more clearly, the content of the present invention will be further elaborated below in combination with experiments. The specific test methods and test results are as follows:

[0007] 1. Screening of microsatellite markers for paternity testing of geese

[0008] 1. Microsatellite marker primer sequence information

[0009] Table 1 Microsatellite marker amplified sequence information

[0010]

[0011]

[0012]

[0013]

[0014] 2. Blood collection

[0015] In Experiment 1, 400 geese flocked, 1 mL of blood was collected from the subwing vein, anticoagulated with sodium citrate, put in 1.5 mL Ep tubes, refrigerated and brought back to the laboratory, stored at -80°C, pending DNA extraction.

[0016] 3. Genomic DNA extraction

[0017] The blood genomic DNA was extracted according to the operation steps of the TIANamp Blood DNA Kit manual, and the DNA quality and purity were tested.

[0...

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Abstract

The invention relates to a method for a goose paternity test. The method uses 20 microsatellite markers with high polymorphism information content and 5 or more alleles, and genomic DNA as an identification material. The identification method comprises carrying out PCR amplification on the 20 microsatellite markers, determining genotypes of the PCR amplification products through a Sanger sequencing method, excluding the parenthood of the offspring and hypothetical parents through an exclusive method when the cumulative probability of exclusion is accumulated to greater than 99% or two or moregenotypes do not accord with the Mendelian inheritance law, respectively calculating the natural logarithms (LOD) of likelihood function ratios of the parents if the two or more hypothetical parents exist after the exclusive method, and comparing the LOD values to determine the possibility of hypothetical parents as true parents of the offspring. The method solves the problem that individual generations of the goose large group during breeding are difficult to identify, produce confusing pedigree information and produce errors.

Description

technical field [0001] The invention relates to the technical field of animal genetics and breeding, in particular to a method for paternity identification of geese. Background technique [0002] Early paternity tests used blood type, serotype, and enzyme type as genetic markers. At present, DNA molecular markers have replaced traditional genetic markers and become the main markers used in paternity testing. Sexual reproduction randomly combines the DNA of both parents, and comparing an individual's DNA sequence with that of another individual can indicate parentage. Microsatellite markers are commonly used DNA molecular markers, with rich polymorphisms, relatively conservative single-copy sequences at both ends of the sequence, highly variable number of alleles, high heterozygosity, good genetic stability, and low mutation rate. Co-dominant inheritance is therefore widely used in the construction of gene maps, monitoring of genetic manipulation effects, determination of g...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12Q1/686
CPCC12Q1/686C12Q1/6869C12Q1/6888C12Q2525/151C12Q2535/101
Inventor 刘胜军宁博林李娥其他发明人请求不公开姓名
Owner HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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