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Human epithelial tissue cancer cell detection reagent and preparation method and application thereof

A detection reagent and epithelial tissue technology, which are applied to the detection reagent of human epithelial tissue cancer cells and the field of preparation thereof, can solve the problems of low specificity, uncommon applicability, poor specificity of acetowhite and the like, and achieve good specificity and low cost. , the effect of high sensitivity

Inactive Publication Date: 2018-07-24
四川天莆高科医疗器械有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, acetic acid white has poor specificity and may easily cause false positives; the iodine test is based on the reaction of glycogen
The specificity is also low; the acridine orange fluorescent staining method requires instrument support, so the applicability is not universal

Method used

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  • Human epithelial tissue cancer cell detection reagent and preparation method and application thereof
  • Human epithelial tissue cancer cell detection reagent and preparation method and application thereof
  • Human epithelial tissue cancer cell detection reagent and preparation method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1 The preparation of detection reagent of the present invention

[0033] 1. Preparation method

[0034] Composition: 90 mL dimethyl sulfoxide (DMSO), 0.5 g folic acid, 0.3 g methylene blue, 6 mL acetic acid, 0.6 g ascorbic acid, 1 g β-cyclodextrin, 0.6 g zinc powder, 0.01 g melatonin.

[0035] step:

[0036] (1) Dissolve 0.5g of folic acid completely in 40mL of DMSO to make solution A;

[0037] (2) Take 0.6g of ascorbic acid, 0.3g of methylene blue, and 0.7g of zinc powder, add 6mL of acetic acid and stir, then add 50mL of DMSO to continue stirring, solution B;

[0038] (3) After the color of solution B fades, add it to solution A, add 1g of β-cyclodextrin and 0.01g of melatonin, and stir to form a solution.

Embodiment 2

[0039] Embodiment 2 Preparation of detection reagent of the present invention

[0040] Composition: 96mL dimethyl sulfoxide, 0.3g folic acid, 0.16g methylene blue, 2mL acetic acid, 0.1g ascorbic acid, 1g β-cyclodextrin, 0.4g zinc powder, 0.04g melatonin.

[0041] step:

[0042] (1) Dissolve 0.3g of folic acid completely in 36mL of DMSO to make solution A;

[0043] (2) Take 0.1g of ascorbic acid, 0.16g of methylene blue, and 0.4g of zinc powder, add 2mL of acetic acid and stir, then add 60mL of DMSO to continue stirring, solution B;

[0044] (3) After the color of solution B faded, it was added to solution A, 1g of β-cyclodextrin and 0.04g of melatonin were added, and stirred to form a solution.

Embodiment 3

[0045] Embodiment 3 Preparation of detection reagent of the present invention

[0046] Composition: 65mL dimethyl sulfoxide, 5g folic acid, 3g methylene blue, 10mL acetic acid, 4.5g ascorbic acid, 5g β-cyclodextrin, 7g zinc powder, 0.5g melatonin.

[0047] step:

[0048] (1) Dissolve 5g of folic acid completely in 50mL of DMSO to make solution A;

[0049](2) Take 5g of ascorbic acid, 3g of methylene blue, 7g of zinc powder, add 10mL of acetic acid and stir, then add 15mL of DMSO to continue stirring, solution B;

[0050] (3) After the color of solution B fades, add it to solution A, add 5g of β-cyclodextrin and 0.1g of melatonin, and stir to form a solution.

[0051] The beneficial effects of the present invention are illustrated below through test examples.

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Abstract

The invention discloses a human epithelial tissue cancer cell detection reagent and a preparation method and application thereof. The human epithelial tissue cancer cell detection reagent is preparedfrom the raw materials in proportions as follows: 0-96 parts of dimethyl sulfoxide by volume, 0-6 parts of a folic acid derivative by weight, 0-3 parts of methylene blue by weight, 0-10 parts of acetic acid by volume, 0-6 parts of ascorbic acid by weight, 0-10 parts of cyclodextrin by weight, 0-10 parts of zinc powder by weight and 0-3 parts of an antioxidant by weight. The invention further provides the preparation method and application of the human epithelial tissue cancer cell detection reagent. The human epithelial tissue cancer cell detection reagent can effectively detect epithelial tissue tumor cells, and has good specificity, high sensitivity and a good application prospect.

Description

technical field [0001] The invention relates to a detection reagent, in particular to a human epithelial cancer cell detection reagent and its preparation method and application. Background technique [0002] In medicine, cancer refers to malignant tumors originating from epithelial tissue, which is the most common type of malignant tumors. In recent years, the morbidity and mortality of tumors in China have continued to rise, which has become a social problem that must be attached great importance to. Many cancers have a high probability of being cured if they are detected early, so the research on early detection methods for malignant tumor cells has become a hot topic in medicine. Examination methods based on pathological cytology and histology were the first to appear, but early cancer cells have not changed in shape and structure, so cytology and histology cannot make a diagnosis. Later, the staining technology of cancer cells developed rapidly. At present, there are ...

Claims

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Application Information

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IPC IPC(8): G01N21/78
CPCG01N21/78G01N2021/775
Inventor 杨成徐伟胡婷婷苏丹钟治晖
Owner 四川天莆高科医疗器械有限公司
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