Bone-targeting drug delivery system based on near-infrared II quantum dots as well as preparation method and application thereof

A drug delivery system and quantum dot technology, applied in the field of biomedicine, can solve the problems of cumbersome preparation method, high cost, inability to achieve bone targeting, etc., and achieve the effects of good tracing effect, small side effects, and beneficial to circulation in the body

Active Publication Date: 2018-08-17
SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The tumor targeting diagnosis and treatment system of the present invention can realize tumor in situ, real-time, visual, quantitative diagnosis, treatment and evaluation, but in order to improve the specificity of targeting tumors, tumor blood vessel targeting molecules need to be coupled, and the preparation method is cumbersome , high cost, and the tumor targeting diagnosis and treatment system of the present invention cannot achieve bone targeting

Method used

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  • Bone-targeting drug delivery system based on near-infrared II quantum dots as well as preparation method and application thereof
  • Bone-targeting drug delivery system based on near-infrared II quantum dots as well as preparation method and application thereof
  • Bone-targeting drug delivery system based on near-infrared II quantum dots as well as preparation method and application thereof

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Experimental program
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Embodiment 1

[0054] 0.1mmol of silver diethyldithiocarbamate and 10g of dodecanethiol were mixed in a flask and placed in a N 2 In the atmosphere, the temperature was raised to 200°C and kept for 1h. Finally, after the solution was naturally cooled to room temperature, 50mL of absolute ethanol was added, centrifuged, washed, dispersed in cyclohexane, 0.15g of lipoic acid was added to the above cyclohexane, and then added The same volume of absolute ethanol was ultrasonicated in an ultrasonic cleaner for 4 hours, then centrifuged, and washed with deionized water to obtain water-soluble Ag particles with a particle size of about 5 nm. 2 S quantum dots; 0.25mg of the above Ag 2 S quantum dots were dispersed in 100 μL dimethyl sulfoxide (DMSO), and then 50 μL DMSO solution containing 0.01 mmol NHS was mixed with the above solution; then 50 μL DMSO solution containing 0.01 mmol EDC was added to the above mixed solution, wrapped with aluminum foil , stirred for 1 h, centrifuged, and then disper...

Embodiment 2

[0055] Example 2 In vitro bone targeting experiment

[0056] Ag with a particle size of 10 nm modified with aminopolyethylene glycol on the surface 2 Mouse femurs were labeled with near-infrared II region quantum dots for 3 hours, then washed with PBS to remove free quantum dots, and imaged under a near-infrared region II fluorescent small animal imager.

Embodiment 3

[0060] Example 3 In Vivo Bone Targeting Experiment

[0061] (1) Preparation of adriamycin bone-targeted drug delivery system

[0062] Dissolve 10 mg of doxorubicin hydrochloride in 1 mL of DMSO, add 6 μL of triethylamine, stir for 2 hours, add 5 mg of Ag with a particle size of 10 nm modified with amino PEG on the surface 2 For quantum dots in the near-infrared II region, add 9 mL of PBS buffer solution, stir at room temperature for 15 hours in the dark, transfer the reaction solution into a 100K ultrafiltration centrifuge tube, centrifuge at 4000 rpm for 30 minutes to remove free doxorubicin, and wash with PBS for 3 times. Obtain the adriamycin bone-targeted drug delivery system (NH 2 -PEG-Ag 2 S-DOX).

[0063] (2) Visualized drug delivery

[0064] Mice were anesthetized by intraperitoneal injection of chloral hydrate solution, NH 2 -PEG-Ag 2 S-DOX by 200 μg Ag 2 The input amount of S / mouse was infused into nude mice through the tail vein, and observed in a near-infrar...

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Abstract

The invention provides a bone-targeting drug delivery system based on near-infrared II quantum dots as well as a preparation method and application thereof. The drug delivery system comprises near-infrared II quantum dots, and the surfaces of the near-infrared II quantum dots are modified by amino polyethylene glycol. The grain size of the near-infrared II quantum dots of the bone-targeting drug delivery system is less than 20nm, and therefore the near-infrared II quantum dots can pass through the blood-marrow barrier to arrive at bone tissues. Since the surfaces of the near-infrared II quantum dots are modified by amino polyethylene glycol, the near-infrared II quantum dots are electropositive, and therefore can be efficiently combined with electronegative hydroxyapatite in bones throughthe action of electrostatic force. The quantum dots only depend on the physical effect to be attached onto the bone tissues by means of kinetics and electrostatic force, achieving the bone-targeting effect, the quantum dots cannot interfere with the therapeutic effect of a drug, and the side effect is minor. Since the carrier is provided with the near-infrared II fluorescent quantum dots, the bone-targeting drug delivery system can track the whole bone targeting process by means of near-infrared II fluorescent signals, which is favorable for the pharmacological research of drugs.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and relates to a bone-targeted drug delivery system based on near-infrared II region quantum dots and a preparation method and application thereof. Background technique [0002] Bones are the frame of the body, and the health of the bones directly affects the survival and quality of life of the body. In recent years, the incidence of bone diseases such as osteoporosis, osteoarthritis and bone tumors has increased year by year, seriously affecting the quality of life of patients. The bone tissue system is huge and widely distributed in the body. During the treatment of bone diseases, if a conventional drug administration method is used, a certain systemic concentration needs to be maintained to allow the drug to reach the tissue and exert its effect, but it may cause systemic disease. Side effects, drug poisoning Treatment options have certain limitations in the treatment of bone diseases. A...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00A61K47/02A61K47/18A61K45/00A61P19/08A61P35/00A61P19/10A61P19/02
CPCA61K45/00A61K47/02A61K47/18A61K49/0054A61K49/0067A61P19/02A61P19/08A61P19/10A61P35/00
Inventor 王强斌陶惠泉
Owner SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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