Enteromorpha polysaccharide, Enteromorpha oligosaccharide, pharmaceutical composition and use thereof
A technology of Enteromorpha polysaccharides and Enteromorpha oligosaccharides, which is applied in the direction of non-glycosyl sugar compounds, sugar derivatives, sugar derivatives, etc., can solve the problem of unreported bactericidal effect, and achieve improved sensitivity and bactericidal range The effect of widening and reducing the amount of usage
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0082] Example 1 Preparation and structural characterization of Enteromorpha polysaccharides and Enteromorpha oligosaccharides
[0083] In this example, the preparation and structural characterization of Enteromorpha polysaccharides and Enteromorpha oligosaccharides were carried out. The specific implementation is as follows:
[0084] A certain quality of Enteromorpha enteromorpha is dried and ground into powder, mixed with 15 to 30 times the weight of water, then added with 0.5% to 2% papain, and reacted at 40 to 70°C for 4 to 6 hours to obtain a reaction solution; the reaction solution The supernatant is collected by centrifugation, and the supernatant is spray-dried at 60-80° C. to obtain a crude dry powder. The crude dry powder is washed with 75% ethanol, centrifuged to obtain the precipitate, and spray-dried to obtain the enteromorpha polysaccharide dry powder. Take a certain amount of dry enteromorpha polysaccharide powder, add 10mg / ml 1M trifluoroacetic acid, hydrolyz...
Embodiment 2
[0094] Example 2 Enteromorpha polysaccharides and Enteromorpha oligosaccharides destroy the effect of Staphylococcus aureus film
[0095] In this example, the destruction effect of Enteromorpha polysaccharides and Enteromorpha oligosaccharides on the biofilm of Staphylococcus aureus was studied. The specific implementation is as follows:
[0096] The wild-type Staphylococcus aureus used was from the China General Microorganism Culture Collection and Management Center. After Staphylococcus aureus was shaken and cultured overnight at 37°C in TSB liquid medium, take 100 μL ~ 2×10 7 The CFU bacterial solution was added to a 96-well plate and cultured statically at 37°C for 24 hours to form a mature bacterial film.
[0097] Enteromorpha polysaccharide, Enteromorpha oligosaccharide, Enteromorpha polysaccharide+streptomycin mixture (mass ratio 1:1), Enteromorpha oligosaccharide+streptomycin mixture (mass ratio 1:1) were prepared for this study. After the bacterial film matured, re...
Embodiment 3
[0103] Example 3 Enteromorpha polysaccharides and Enteromorpha oligosaccharides destroy effect experiment on Pseudomonas aeruginosa bacterial film
[0104] In this example, the destruction effect of Enteromorpha polysaccharides and Enteromorpha oligosaccharides on the bacterial film of Pseudomonas aeruginosa was studied. The specific implementation is as follows:
[0105] The wild-type Pseudomonas aeruginosa PAO1 used was from the China General Microorganism Culture Collection and Management Center. After Pseudomonas aeruginosa was shaken and cultured overnight at 37°C in LB liquid medium, take 100 μL ~ 2×10 7 The CFU bacterial solution was added to a 96-well plate and cultured statically at 30°C for 24 hours to form a mature bacterial film.
[0106]Enteromorpha polysaccharides and Enteromorpha oligosaccharides were prepared separately for this study. After the bacterial film matured, the supernatant liquid was removed, and 100 μL of LB liquid medium containing 250 μg / mL of...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More