Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A bacterial agent for degrading triazine herbicides and its preparation method

A technology of herbicides and triazines, which is applied in the field of triazine herbicide-degrading bacterial agents and its preparation, can solve problems such as residual pollution of triazine herbicides, and achieve great biological restoration significance, economic benefits, and production costs Low, damage-reducing effect

Active Publication Date: 2021-08-24
ANHUI AGRICULTURAL UNIVERSITY
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve problems such as residual pollution of triazine herbicides, the object of the present invention is to provide a bacterial agent for degrading triazine herbicides and a preparation method

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A bacterial agent for degrading triazine herbicides and its preparation method
  • A bacterial agent for degrading triazine herbicides and its preparation method
  • A bacterial agent for degrading triazine herbicides and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The operation steps for preparing the bacterial agent for degrading triazine herbicides are as follows:

[0038] (1) Prepare Pseudomonas stutzeri (Pseudomonas stutzeri sp.) aau-3 wet thallus

[0039]Under sterile conditions, a small amount of Pseudomonas stutzeri sp. aau-3 was inoculated in a solid medium with an inoculation loop, and placed in a constant temperature incubator for 3 days in the dark, at a temperature of 30 ℃±1℃, to get activated strains; inoculate two loops of activated strains into 100mg L of drug-containing -1 , in a 250mL Erlenmeyer flask with 100mL liquid enrichment medium, cultured in a constant temperature shaker at 30°C±1°C and 150r / min for 36h to obtain a bacterial suspension; inoculate 2mL of this bacterial suspension into a 100mg L -1 , 300mL of liquid culture medium in a 1000mL Erlenmeyer flask, 30°C±1°C, 150r / min constant temperature shaker for 36h, the bacterial suspension was centrifuged at 6000r / min for 10min, the supernatant was discard...

Embodiment 2

[0060] Degradation experiment of simazine by bacterial agent degrading triazine herbicides

[0061] 1 Degradation experiment of bacterium agent on simazine in water body

[0062] (1) Preparation of Mineral Salt Medium (hereinafter referred to as MSM):

[0063] MgSO 4 ·7H 2 O, 0.4 g; FeSO 4 ·7H 2 O, 0.002 g; K 2 HP0 4 , 0.2 g; (NH4) 2 8O 4 , 0.2 g; CaSO 4 , 0.08 g; 1 L of distilled water; adjusted to pH 7, autoclaved at 121°C and 101KPa for 20 minutes for later use.

[0064] (2) In 20 mL of MSM containing 100.0 mg / L simazine, add 0.5 g of the prepared triazine herbicide-degrading bacteria agent, and cultivate 0, 3, 6, After 9, 12, 16, and 24 hours, extract and detect the residual concentration of simazine. At the same time, the microbial agent of the present invention without humidifying the bacteria was set as a blank control, and three repetitions were set for both the control and the treatment.

[0065] Experimental results such as figure 2 It can be seen that ...

Embodiment 3

[0078] (1) Degradation experiments of bacterial agents on other triazine herbicides

[0079] To the 20mL MSM containing 50.0mg / L atrazine, terbuthine, propazine, atrazine, proprazine, atrazine and terbutyne respectively, add 0.5g of bacterial agent for degrading triazine herbicides, After culturing on a shaker at 30°C and 150r / min for 2 and 4 days, extract and detect the residual concentration of triazine herbicides. At the same time, the microbial agent of the present invention without humidifying the bacteria was set as a blank control, and three repetitions were set for both the control and the treatment.

[0080] The degradation of other triazine herbicides by the bacterial agent of table 4

[0081]

[0082]

[0083] The results are shown in Table 4. The bacterial agent for degrading triazine herbicides has a good degradation effect on atrazine, terbuthine, and permethazine. After 2 days, the degradation rate of atrazine reached 99%. The degradation rate was more t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a fungicide for degrading triazine herbicides. The bacterial agent takes sodium alginate, polyvinyl alcohol, and activated carbon as carriers, and embeds Pseudomonas stutzeri ( Pseudomonas stutzeri sp. ) aau-3; in the bacterial agent, the wet cell content of Pseudomonas stutzeri aau-3 bacteria is 20 g / L, the polyvinyl alcohol content is 140 g / L, and the sodium alginate content is 10 g / L; the bacterial agent is spherical and granular, with smooth surface, good elasticity, good mechanical strength and good permeability. Pseudomonas stutzeri aau-3 has good degradation effect on several triazine herbicides, and can rapidly degrade triazines such as atrazine and terbumine containing chlorine group (-Cl) herbicide. Free bacteria are easily lost in the environment and have poor environmental adaptability. For this reason, it is necessary to apply the bacteria to the water or soil contaminated by triazine herbicides in actual production. It has great significance and economic benefits.

Description

technical field [0001] The invention belongs to the technical field of microbial remediation of organic pollution, and includes a triazine herbicide-degrading bacterial agent and its preparation method and application. Background technique [0002] The triazine herbicide simazine (2-chloro-4,6-Bis(ethylamino)-1,3,5-triazine) is a systemic selective herbicide, which has teratogenic and growth-affecting toxic effects on animals and plants , but due to its excellent effect of controlling farmland broad-leaved grass and gramineous grasses, its usage is increasing day by day, and the environmental problems caused by simazine also follow. In this patent, a simazine efficient degrading bacterium screened from the sewage of a pesticide factory is used for immobilization research. Through the selection of fixation methods and materials, the optimal conditions for the preparation of bacterial agents were studied for repeated use experiments and the determination of degradation spectr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N11/10C12N11/084C12N11/14C02F3/34A62D3/02C02F101/30C12R1/38
CPCA62D3/02C02F3/34C02F2101/306C12N1/20C12N11/08C12N11/10C12N11/14C12N1/205C12R2001/38
Inventor 吴祥为潘丹丹闫涛杨雪颖花日茂
Owner ANHUI AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com