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A kind of effective part of Lonicera vine mushroom with anti-tumor effect and its preparation method and application

An effective part, the technology of honeysuckle rattan mushroom, applied in the field of natural medicinal chemistry, to achieve strong effect, enhanced anti-tumor effect, and strong anti-breast cancer effect

Active Publication Date: 2021-05-14
LINYI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, there is no report that the effective parts of Lonicera vine mushroom can treat breast cancer

Method used

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  • A kind of effective part of Lonicera vine mushroom with anti-tumor effect and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 The preparation method of the effective part of Lonicera vine mushroom with anti-tumor effect

[0031] (1) get Lonicerae vine (collected from Linyi, Shandong), use 95% ethanol by volume fraction to carry out heating and reflux extraction for 2h to obtain extract, concentrate the extract to obtain Lonicera alcohol extract; The dosage ratio is 12mL: 1g;

[0032] (2) Put the alcoholic extract of Lonicerae vine into the D101 macroporous resin column, first use 2 times the volume fraction of the column volume to elute and remove impurities with ethanol aqueous solution of 50%; then use 4 times the volume fraction of the column volume to be 75% ethanol aqueous solution; collect the eluting part with a volume fraction of 75% ethanol aqueous solution, concentrate and dry to obtain the effective part of macroporous resin column chromatography, that is, the effective part of Lonicera vine mushroom.

Embodiment 2

[0033] Example 2 The preparation method of the effective part of Lonicera vine mushroom with anti-tumor effect

[0034] (1) get Lonicera vine (collected from Linyi, Shandong), carry out heating and reflux extraction with 80% ethanol for 2h to obtain extract, concentrate the extract to obtain Lonicera alcohol extract; described ethanol and Lonicera vine alcohol extract The dosage ratio is 15mL: 1g;

[0035] (2) Put the alcoholic extract of Lonicerae vine into the D101 macroporous resin column, first use 3 times the volume fraction of the column volume to elute and remove impurities with ethanol aqueous solution of 40%; then use 3 times the volume fraction of the column volume to be 70% ethanol aqueous solution; collect the eluting part with a volume fraction of 70% ethanol aqueous solution, concentrate and dry to obtain the effective part of macroporous resin column chromatography, that is, the effective part of Lonicerae vine.

Embodiment 3

[0036] Example 3 The preparation method of the effective part of Lonicera vine mushroom with anti-tumor effect

[0037] (1) get Lonicerae vine (collected from Linyi, Shandong), heat and reflux extraction with 70% ethanol for 3h to obtain extract, concentrate the extract to obtain Lonicera alcohol extract; described ethanol and Lonicera vine alcohol The dosage ratio is 15mL: 1g;

[0038] (2) Put the alcoholic extract of Lonicerae vine into the D101 macroporous resin column, first use 1 times the volume fraction of the column volume as 60% ethanol aqueous solution to remove impurities; then use 5 times the volume fraction of the column volume as 80% ethanol aqueous solution; collect the eluted part with a volume fraction of 80% ethanol aqueous solution, concentrate and dry to obtain the effective part of macroporous resin column chromatography, that is, the effective part of Lonicerae vine.

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PUM

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Abstract

The invention relates to the technical field of natural medicinal chemistry, and specifically discloses an effective part of the honeysuckle mushroom with anti-tumor effect and a preparation method and application thereof. The method comprises the following steps: (1) taking the honeysuckle mushroom, extracting with alcohol, and concentrating the extract to obtain the honeysuckle mushroom alcohol extract; (2) subjecting the honeysuckle mushroom alcohol extract to macroporous resin column chromatography to obtain The effective part of macroporous resin column chromatography; wherein, the specific method of carrying out macroporous resin column chromatography in step (2) is: placing the alcohol extract of Lonicera japonica on the macroporous resin column, first using 1 to 3 times the column volume of The volume fraction of 40-60% ethanol aqueous solution is used for elution to remove impurities; then 1 to 5 times the column volume is used for elution with the volume fraction of 70-80% ethanol aqueous solution; the collection volume fraction is 70-80% ethanol aqueous solution for elution Parts, concentrated and dried to obtain effective parts of macroporous resin column chromatography. The effective part of the honeysuckle mushroom prepared by the method has excellent anti-tumor effect.

Description

technical field [0001] The invention relates to the technical field of natural medicine chemistry, in particular to an effective part of honeysuckle vine mushroom with anti-tumor effect and its preparation method and application. Background technique [0002] Breast cancer is a malignant tumor that occurs in the glandular epithelial tissue of the breast. Female mammary glands are composed of skin, fibrous tissue, mammary glands and fat. 99% of breast cancers occur in women, and only 1% in men. The global incidence of breast cancer has been on the rise since the late 1970s. Breast cancer has become a major public health problem in the current society. Therefore, it is of great significance to further develop a drug capable of treating breast cancer. [0003] Lonicerae vine is a fungus of the Polyporaceae Phytophthora genus. Its scientific name is Xanthochrous nilgheriensis (Mont.) Teng. Shape stacked, mainly distributed in Yimeng Mountains, Shandong Province, Liaoning Pro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/07A61P35/00
CPCA61K36/07A61K2236/33A61K2236/51A61K2236/55A61P35/00
Inventor 杨文强
Owner LINYI UNIVERSITY