Biological glue based on polyhedral oligomeric silsesquioxane modified polyethylene glycol, preparation method and applications thereof

A technology of polysilsesquioxane and polyethylene glycol, which is applied in the fields of application, medical science, surgery, etc., and can solve problems such as wound dehiscence

Active Publication Date: 2018-08-21
深圳宁聚生物新材料科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the hydrophilic property of polyethylene glycol is too good, it will swell in body fluids, and when used as a component of bioglue, it will cause wound cracking
This limits the clinical application of polyethylene glycol bioglue, so it is necessary to improve the swelling properties of polyethylene glycol

Method used

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  • Biological glue based on polyhedral oligomeric silsesquioxane modified polyethylene glycol, preparation method and applications thereof
  • Biological glue based on polyhedral oligomeric silsesquioxane modified polyethylene glycol, preparation method and applications thereof
  • Biological glue based on polyhedral oligomeric silsesquioxane modified polyethylene glycol, preparation method and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0099] Weigh 4 g of octavinyl polyhedral oligomeric silsesquioxane, dissolve it in 250 ml of dichloromethane, then add 530 μl of methanesulfonic acid therein, and stir overnight at room temperature. After washing 3 times with saturated sodium bicarbonate and saturated brine respectively, the organic phase was concentrated, diluted with acetone, added 10ml of water to react overnight, then concentrated by rotary evaporation, and purified by carbon 18 reverse silica gel column to obtain monohydroxyl-substituted Polyhedral oligomeric silsesquioxane.

[0100] Take 1 mol of four-arm polyethylene glycol carboxyl group with a molecular weight of 10000 and dissolve it in 100 ml of anhydrous dichloromethane, add 4 mol of polyhedral oligomeric silsesquioxane substituted by monohydroxyl, 1 mol of 4-dimethylaminopyridine and 4 mol of 1 -(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, react overnight at 37°C, wash with pH 1 hydrochloric acid and saturated brine for 3 times, and ...

Embodiment 2

[0104] Take the precursor (as shown in formula II, the structural formula is as figure 1 Shown in the structural formula, wherein n is 56, R 1 is the ester bond of succinates (shown in formula a), R 2 Middle R a , R b , R c , R d , R e , R f All are amide bonds of β-sulfides (shown in formula r), R 3 It is also the amide bond of β-sulfides (shown in formula b), R 4 is a catechol group, m is 7) 200mg is dissolved in 2mL PBS buffer solution, 5mg sodium periodate is added to 5mL aqueous solution, and then 2mL precursor solution is mixed with 1mL sodium periodate solution to obtain bioglue .

[0105]

[0106] The bioglue that obtains is carried out adhesion performance measurement, and adhesion strength is 30kPa, is better than commercially available fibrin glue, as figure 2 shown.

[0107] The adhesion performance is measured by pigskin bonding and stretching method: the biological glue precursor and sodium periodate solution are respectively coated on two pieces o...

Embodiment 3

[0109] Take the precursor (as shown in formula I, wherein n is 56, R 1 is a straight-chain ether bond containing two carbon atoms, R 2 Middle R a , R b For α-thioether carboxyl (as shown in formula c), R c , R d , R e , R f All are ester bonds of α-sulfides (as shown in formula d), R 3 is the ester bond of α-sulfides (as shown in formula d), R 4 is a catechol group, m is 6) 100mg is dissolved in 5mL PBS buffer solution, 5mg sodium periodate is added to 7mL aqueous solution, and then 2mL precursor solution is mixed with 1mL sodium periodate solution to obtain bioglue .

[0110]

[0111] The bioglue that obtains is carried out adhesion performance measurement, and adhesion strength is 45kPa, and swelling rate is 166.2%, reduces greatly compared to original swelling rate, as image 3 shown.

[0112] The method of measuring the swelling rate is as follows: take a certain weight of biological glue, place it in a vacuum oven at 40°C for 24 hours, and ensure that the moi...

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Abstract

The invention discloses a biological glue based on polyhedral oligomeric silsesquioxane modified polyethylene glycol, a preparation method and applications thereof. According to the present invention,polyhedral oligomeric silsesquioxane modified polyethylene glycol and a cross-linking agent are subjected to a chemical reaction or hydrogen bonding interaction in a dissolution medium to form the biological glue, wherein the structure formula of the polyhedral oligomeric silsesquioxane modified polyethylene glycol is represented by a formula I, a formula II, a formula III or a formula IV, and the cross-linking agent is sodium periodate, fibrinogen, ferric chloride, ferric sulfate, hydrogen peroxide, a hydrogen peroxide / catalase system, oxygen or ozone; and the polyhedral oligomeric silsesquioxane (POSS) enters the terminal of the polyethylene glycol (PEG), and the polyhedral oligomeric silsesquioxane has good biocompatibility, can promote cell adhesion and cell growth, and further has advantages of high functionality and strong hydrophobicity, such that the biological glue with characteristics of strong adhesion, good biocompatibility, no swelling and promotion of cell growth and tissue regeneration is obtained.

Description

technical field [0001] The invention relates to a wound adhesive and its preparation method and application, in particular to a bioglue based on polyhedral oligomeric silsesquioxane modified polyethylene glycol and its preparation method and application. Background technique [0002] Surgery has become an important means of diagnosis and treatment of various diseases. Wound suturing is an essential means of treatment in surgical operations. Surgical sutures are still used for traditional wound closure. Surgical sutures present a number of problems. First of all, suturing will cause wound expansion; most sutures are non-degradable sutures, which will hinder wound healing, and taking out sutures will cause secondary injury to the wound; Influenced by the operator's suturing technique. So people have developed a sealant for wound adhesion. Adhesives can generally be divided into two categories: natural materials and synthetic materials. Natural material adhesives are repr...

Claims

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Application Information

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IPC IPC(8): C08G77/46A61L24/04A61L24/00
CPCA61L24/001A61L24/046C08G77/46C08L83/12
Inventor 吴德成补亚忠杨飞
Owner 深圳宁聚生物新材料科技有限公司
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