Polygene element realizing equivalent expression of two genes based on 2A lytic peptide, expression vector and application

A technology of genetic elements and expression vectors, applied in the direction of vectors, nucleic acid vectors, and the use of vectors to introduce foreign genetic material, etc.
CN108486113AActive Publication Date: 2018-09-04SOUTHWEST UNIVERSITY
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Applications(China)
Current Assignee / Owner
SOUTHWEST UNIVERSITY
Publication Date
2018-09-04

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Abstract

The invention relates to a polygene element realizing equivalent expression of two genes based on 2A lytic peptide, an expression vector and application. The gene element is formed by Ck and the 2A lytic peptide, wherein a nucleotide sequence of the 2A lytic peptide is as shown in SEQ ID NO.38, and a nucleotide sequence of the Ck is as shown in SEQ ID NO.39. The vector containing the element can be used for equivalently performing recombination expression on the genes located on two ends of 2A so as to have significance on realizing silkworm poly-gene expression.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, and relates to a multigene element based on 2A cleavage peptide for realizing the equal expression of two genes, and also relates to an expression vector containing the element and its application. Background technique

[0002] Efficient recombinant expression of multiple genes in silkworms can promote basic and applied research in silkworms, such as studying the relationship between multiple genes, recombinantly expressing genes of multi-subunit protein complexes, and simultaneously expressing fluorescent proteins to visualize targets The movement of genes in the body and the breeding of silkworm varieties resistant to silkworm cytoplasmic polyhedrosis virus and nuclear polyhedrosis virus, etc. Thanks to the establishment of the silkworm transgenic operating system in 2000, previously, simultaneous expression of multiple genes in the silkworm was mainly achieved through genetic hybridization. First...

Claims

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