Female bacteriostatic moisturizing care solution with anticancer effect and preparation method thereof
A care solution, technology for women
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[0031] (1) Preparation of drug-sensitive tablets: Take sterile and dry filter paper pieces with a diameter of 3 cm, add 20 μL of female antibacterial moisturizing care solution with anticancer effect to each piece, then place the filter paper pieces flat in a clean sterile plate, and dry them. reserve;
[0032] (2) Preparation of negative control samples: Take sterile dry filter paper pieces with a diameter of 3 cm, add 20 μL of sterile distilled water dropwise to each piece, and dry it for later use;
[0033] (3) Drug susceptibility test by K-B disk diffusion method: use a sterile cotton swab to dip in a concentration of 5×10 6 cfu / ml test bacteria suspension, squeeze out the excess bacteria solution on the wall of the tube, spread the entire surface of the nutrient agar medium plate, and repeat 3 times; each time the plate is smeared, the plate is rotated 60 degrees to spread evenly, and finally wrap the cotton swab around Smear on the edge of the plate for a week; cover th...
Embodiment 1
[0035] The antibacterial moisturizing care solution for women with anticancer effect is prepared from the following raw materials in mass percentage: 0.4% hydrolyzed soybean, 0.4% hydrolyzed oat, 0.4% menthol, and the balance is deionized water.
[0036]The preparation method of the hydrolyzed oats is as follows: crush oats through a 30-mesh sieve to obtain oat flour; add oat flour to deionized water to prepare a stock solution with a mass fraction of oat flour of 15%, and adjust the stock solution with 0.5mol / L acetic acid pH to 1.5, then add chitosan and mix evenly to obtain a slurry, the mass fraction of the chitosan in the slurry is 0.2%, the slurry is stirred at a speed of 200r / min for 50min, and filtered with a 300-mesh filter cloth to obtain Hydrolyzed solution; the hydrolyzed solution was concentrated under reduced pressure at an absolute pressure of 0.06 MPa and a temperature of 55° C., and the concentration time was 6 hours to obtain a concentrated solution; the conce...
Embodiment 2
[0040] The antibacterial moisturizing care solution for women with anticancer effect is prepared from the following raw materials in mass percentage: 0.4% hydrolyzed soybean, 0.4% hydrolyzed oat, 0.4% menthol, and the balance is deionized water.
[0041] The preparation method of the hydrolyzed oats is as follows: crush oats through a 30-mesh sieve to obtain oat flour; add oat flour to deionized water to prepare a stock solution with a mass fraction of oat flour of 15%, and adjust the stock solution with 0.5mol / L acetic acid pH to 1.5, then add chitosan and mix evenly to obtain a slurry, the mass fraction of the chitosan in the slurry is 0.2%, the slurry is subjected to ultrasonic treatment for 50min, the ultrasonic power of the ultrasonic treatment is 250W, and the ultrasonic frequency is 35kHz , using a 300-mesh filter cloth to filter to obtain a hydrolyzate; the hydrolyzate was concentrated under reduced pressure at an absolute pressure of 0.06MPa and a temperature of 55°C, ...
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