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A new type of fermenting lactobacillus and its application in the field of feed and sewage treatment

A fermented lactobacillus, a new type of technology, applied in the field of microorganisms, can solve problems such as poor salt tolerance and easy inactivation, and achieve the effects of increasing intestinal activity, reducing malodorous substances, and promoting digestion and metabolism

Active Publication Date: 2020-12-25
浙江亲水园生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Aiming at the problems that existing lactobacilli have poor acid resistance, salt resistance, and easy inactivation, the present invention provides a new type of fermenting lactobacillus and its application in the field of feed and sewage treatment. The fermenting lactobacillus LBF128 of the present invention has Acid resistance, salt resistance, high temperature resistance, strain LBF128 or its mutants that can reproduce under anaerobic, aerobic and facultative conditions, acid resistance means that it can survive for more than 2 hours in an environment with a pH as low as 2.0. Salt performance refers to the ability to survive and function in environments with up to 6% salt

Method used

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  • A new type of fermenting lactobacillus and its application in the field of feed and sewage treatment
  • A new type of fermenting lactobacillus and its application in the field of feed and sewage treatment
  • A new type of fermenting lactobacillus and its application in the field of feed and sewage treatment

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Acid resistance test

[0058] Experimental process: LBF128 lactic acid bacteria were cultivated in a petri dish with diluted agar medium, and the content of LBF128 lactic acid bacteria in the petri dish was adjusted to 10 7 / mL, the pH values ​​of the medium were adjusted to 3.0 and 2.0 with dilute hydrochloric acid, respectively. After cultivating for 1 hour and 2 hours, respectively detect the content of LBF128 lactic acid bacteria in the petri dish, the results are as follows:

[0059] Experimental results

[0060] The acid resistance test result of table 1 lactobacillus

[0061]

[0062] The above experiments prove that LBF128 lactic acid bacteria have strong acid resistance and can survive for more than 2 hours in an environment of pH=2.0. Under acidic conditions of pH=3.0, LBF128 lactic acid bacteria can not only survive but also maintain reproductive capacity. Therefore, it is considered that it can basically pass through the strongly acidic stomach enviro...

Embodiment 2

[0064] Salt tolerance test

[0065] Experimental process: LBF128 lactic acid bacteria were cultivated in a petri dish with diluted agar medium, and the content of LBF128 lactic acid bacteria in the petri dish was adjusted to 10 7 / mL, then adjust the saline environment containing salinity 6% (mass concentration) with salt, cultivate the LBF128 lactic acid bacteria content in the culture dish after 2 hours and 4 hours respectively, the results are as follows:

[0066] Salt tolerance experiment result of table 2 lactobacillus

[0067]

[0068] The above experiment proves that LBF128 lactic acid bacteria have strong salt resistance ability, and can survive for more than 4 hours in an environment with a salt concentration as high as 6%, indicating that LBF128 lactic acid bacteria of the present invention can survive and work in high salinity water such as seawater.

Embodiment 3

[0070] High temperature test

[0071] Experimental process: LBF128 lactic acid bacteria were cultivated in a petri dish with diluted agar medium, and the content of LBF128 lactic acid bacteria in the petri dish was adjusted to 10 7 / mL, then place the petri dish in an environment of 60°C and 80°C for cultivation, and detect the content of LBF128 lactic acid bacteria in the petri dish after cultivating for 1 hour and 2 hours, the results are as follows:

[0072] Salt tolerance experiment result of table 3 lactobacillus

[0073]

[0074] The above experiments prove that LBF128 lactic acid bacteria have a strong ability to resist high temperature, and can still survive and work in a high temperature environment of 80°C.

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Abstract

The invention discloses a novel fermenting lactobacillus and its application in the fields of feed and sewage treatment, belonging to the field of microorganisms. The novel fermenting Lactobacillus of the present invention is Lactobacillus Fermentum bacterial strain LBF128, has acid-resistant, salt-resistant, high-temperature-resistant performance, the characteristic that all can breed under anaerobic, aerobic and facultative state, under the condition of pH=2.0, still can Survive for more than 2 hours, and can reproduce under anaerobic, aerobic and facultative conditions, so it can keep more than 70% of live bacteria after passing through the stomach and reach the intestinal tract. It can be used as an additive for livestock and poultry feed, and can also be used for feces The biological deodorization has a stable effect, and can degrade the pollutants in the original feces and urine (COD above 25000) to below 1500; it can also be used for the treatment of domestic sewage, acidic high-salt sewage, and river basin sewage, with good acid and salt resistance , high survival rate, remarkable treatment effect, and can still survive and work in an environment with a salt content as high as 6%.

Description

technical field [0001] The invention belongs to the field of microorganisms, and more specifically relates to a novel fermenting lactobacillus and its application in the fields of feed and sewage treatment. Background technique [0002] Lactobacillus refers to bacteria that can ferment sugar to produce lactic acid. It is a group of microorganisms that live in the body and benefit the health of the host. Its role in maintaining human health and regulating immune function has been widely recognized. Lactobacillus is a genus of Lactobacillus family, named for the large amount of lactic acid produced by fermenting sugar. Widely distributed in nature, it is one of the important physiological flora in the intestinal tract of animals and humans. The vast majority of bacteria in this group are non-toxic and harmless to animals and humans, and they are responsible for important physiological functions in animals. Among lactic acid bacteria, Lactobacillus is the largest genus, which...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K10/18C02F3/34C12R1/225
CPCA23K10/18C02F3/34C12N1/20C12N1/205C12R2001/225
Inventor 丁庆
Owner 浙江亲水园生物科技有限公司
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