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Method for breeding pigs achieving high feed utilization efficiency

A feed and high-efficiency technology, applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve expensive and time-consuming problems, and achieve the effects of low cost, high feed utilization efficiency, and high application value

Active Publication Date: 2018-09-21
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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AI Technical Summary

Problems solved by technology

[0003] Estimates of residual feed intake require a large amount of average daily feed intake data, although newer electronic feeding equipment and continuous development of feed intake measurement systems have made feed intake data collection more convenient, but are still expensive and time consuming

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  • Method for breeding pigs achieving high feed utilization efficiency

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Experimental program
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Embodiment 1

[0023] Example 1. Auxiliary identification of pig residual feed intake traits

[0024] 1. Determination of pig g.13.200546552A>G polymorphism

[0025] 1. PCR amplification

[0026] According to the deoxyribonucleotide information at position 200546552 on chromosome 13 of the reference sequence of the International Porcine Genome Version 11.1, a pair of primers were designed as follows:

[0027] U (upstream primer): 5'-TCCCTTGGCACAACATGGAT-3' (SEQ ID NO: 1 of the sequence listing);

[0028] D (downstream primer): 5'-TTACAAAGAGCAGGTGGAGGC-3' (SEQ ID NO: 2 of the Sequence Listing).

[0029] Duroc pigs (2 heads) were selected as experimental materials. PCR amplification was performed using primers U and D using the genomic DNA of Duroc pigs as a template.

[0030] The amplification system was: genomic DNA 200ng, 10× PCR amplification buffer 5μl, dNTPs 10mM, upstream and downstream primers 50ng each, Taq DNA polymerase 0.75U, Mg 2+ 2.5mmol / L, with ddH 2 O replenish the reacti...

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Abstract

The invention discloses a method for breeding pigs achieving the high feed utilization efficiency. The method comprises the steps that whether the 200546552 deoxyribonucleotide on a chromosome 13of an international pig genome 11.1 version reference sequence of a to-be-detected pig is A or G is detected, whether genotype of the to-be-detected pig is AA or GG is determined, and the residual food intake of the pig of the GG genotype is lower than that of the pig of the AA genotype; the GG genotype is homozygote that the 200546552 deoxyribonucleotide on the chromosome 13 of the international pig genome 11.1 version reference sequence is G; the AA genotype is homozygote that the 200546552 deoxyribonucleotide on the chromosome 13 of the international pig genome 11.1 version reference sequence is A. By applying the method, the breeding cost can be lowered, pig feed utilization efficiency in actual production is effectively improved, and the economical efficiency is improved. Themethod is easy to implement, high in accuracy and capable of achieving automatic detection, and the method plays a huge role in pig breeding.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for cultivating pigs with high feed utilization efficiency. Background technique [0002] In pig production, feed input is the largest, accounting for about 70% of the total breeding cost. Therefore, feed utilization efficiency has received more and more attention, and it has become one of the important selection target traits in pig breeding. The evaluation indicators of feed utilization efficiency mainly include feed weight gain ratio, weight gain feed ratio and residual feed intake (RFI). Residual feed intake is the difference between the actual feed intake of livestock and poultry and the predicted feed intake required for maintenance and weight gain, which can reflect the metabolic differences of animals themselves determined by genetic background (Foster W, Kilpatrick D, Heaney I. Genetic variation in the efficiency of energy utilization by the fattening pig. Animal ...

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Application Information

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IPC IPC(8): C12Q1/6888
CPCC12Q1/6888C12Q2600/156C12Q2600/124
Inventor 张龙超王立贤王立刚张跃博
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI